##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633207.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	405925
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87376485804028	33.0	33.0	33.0	33.0	33.0
2	31.978518199174726	33.0	33.0	33.0	33.0	33.0
3	31.70389111289031	33.0	33.0	33.0	27.0	33.0
4	31.984497136170475	33.0	33.0	33.0	33.0	33.0
5	32.087193447065346	33.0	33.0	33.0	33.0	33.0
6	35.24806799285582	37.0	37.0	37.0	33.0	37.0
7	35.38054074028454	37.0	37.0	37.0	33.0	37.0
8	35.53383753156371	37.0	37.0	37.0	33.0	37.0
9	35.65197265504712	37.0	37.0	37.0	33.0	37.0
10	35.66831311202808	37.0	37.0	37.0	33.0	37.0
11	35.669337931883966	37.0	37.0	37.0	33.0	37.0
12	35.63179405062512	37.0	37.0	37.0	33.0	37.0
13	35.66405370450207	37.0	37.0	37.0	33.0	37.0
14	35.650068362382214	37.0	37.0	37.0	33.0	37.0
15	35.65558662314467	37.0	37.0	37.0	33.0	37.0
16	35.640246350926894	37.0	37.0	37.0	33.0	37.0
17	35.625297776682885	37.0	37.0	37.0	33.0	37.0
18	35.62455626039293	37.0	37.0	37.0	33.0	37.0
19	35.61744164562419	37.0	37.0	37.0	33.0	37.0
20	35.61132967912792	37.0	37.0	37.0	33.0	37.0
21	35.60349818316191	37.0	37.0	37.0	33.0	37.0
22	35.52106177249492	37.0	37.0	37.0	33.0	37.0
23	35.555592781917845	37.0	37.0	37.0	33.0	37.0
24	35.54311264396132	37.0	37.0	37.0	33.0	37.0
25	35.57599063866478	37.0	37.0	37.0	33.0	37.0
26	35.46956211122745	37.0	37.0	37.0	33.0	37.0
27	35.47224979983987	37.0	37.0	37.0	33.0	37.0
28	35.50048654308062	37.0	37.0	37.0	33.0	37.0
29	35.499769661883356	37.0	37.0	37.0	33.0	37.0
30	35.50488883414424	37.0	37.0	37.0	33.0	37.0
31	35.49320687319086	37.0	37.0	37.0	33.0	37.0
32	35.482525097000675	37.0	37.0	37.0	33.0	37.0
33	35.47262918026729	37.0	37.0	37.0	33.0	37.0
34	35.435772618094475	37.0	37.0	37.0	33.0	37.0
35	35.37822504157172	37.0	37.0	37.0	33.0	37.0
36	35.38472377902322	37.0	37.0	37.0	33.0	37.0
37	35.3775204779208	37.0	37.0	37.0	33.0	37.0
38	35.36298577323397	37.0	37.0	37.0	33.0	37.0
39	35.26002340333805	37.0	37.0	37.0	33.0	37.0
40	35.044094352405004	37.0	37.0	37.0	27.0	37.0
41	35.207612243641066	37.0	37.0	37.0	33.0	37.0
42	35.235706103344214	37.0	37.0	37.0	33.0	37.0
43	34.91402352651352	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	16.0
17	15.0
18	14.0
19	11.0
20	33.0
21	69.0
22	167.0
23	402.0
24	818.0
25	1501.0
26	2608.0
27	3919.0
28	5512.0
29	7527.0
30	10121.0
31	12694.0
32	16511.0
33	21580.0
34	31134.0
35	62953.0
36	228305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78130196464864	20.911005727659052	13.400997721253926	22.90669458643838
2	16.32148795959845	22.598509576892283	38.823674324074645	22.256328139434626
3	18.01170166902753	27.75512717866601	29.931144915932745	24.302026236373713
4	11.820163823366386	18.19104514380735	40.38504649873745	29.60374453408881
5	13.950606639157478	36.767136786352154	35.01090102851512	14.271355545975242
6	28.513888033503726	40.75900720576461	17.481308123421815	13.245796637309848
7	27.054751493502494	32.414362259038	22.854714540863462	17.676171706596048
8	24.542711091950483	34.90965079756113	20.933177311079636	19.614460799408757
9	25.904046313974256	14.832050255589085	20.52866908911745	38.735234341319206
10	15.160682392067502	27.970437888772558	33.74613536983433	23.122744349325615
11	33.765597093059064	24.578431976350313	23.69871281640697	17.957258114183656
12	23.052041633306644	25.795159204286506	30.694832789308368	20.45796637309848
13	28.140912730184148	23.028884646178483	26.45513333743918	22.37506928619819
14	22.123544989838024	21.801071626531996	28.385292849664346	27.690090533965634
15	23.491778037814868	29.18026729075568	26.048407957134938	21.27954671429451
16	22.82860134261255	28.834636940321488	25.417749584282813	22.919012132783152
17	22.559093428589026	27.20428650612798	26.1035905647595	24.133029500523495
18	22.35215864999692	26.321611135061897	29.328324197819793	21.997906017121387
19	25.252448112336022	26.206318901274866	28.34094968282318	20.20028330356593
20	24.66145223871405	26.103836915686397	28.433823982262734	20.800886863336824
21	22.411036521524913	27.292972839810307	28.365338424585822	21.930652214078954
22	23.02050871466404	26.181930159512223	28.59764734864815	22.19991377717559
23	21.983371312434564	26.661575414177495	29.42267660282072	21.932376670567223
24	22.10137340641744	27.613475395701176	27.84332081049455	22.441830387386833
25	22.310032641497813	27.589086653938537	28.977766828847695	21.12311387571596
26	23.584898688181312	26.719221531071007	27.9044158403646	21.79146394038308
27	23.039724086961876	26.72464125146271	27.56124899919936	22.674385662376054
28	22.102851511978812	26.63521586499969	29.05364291433147	22.208289708690028
29	23.532425940752603	27.211430683007944	27.976596661944942	21.27954671429451
30	23.194925170905954	26.507359733940998	28.16209890989715	22.135616185255895
31	22.37827184824783	26.096200036952638	28.747182361273634	22.778345753525898
32	21.36675494241547	26.901521216973578	28.68214571657326	23.04957812403769
33	21.939520847447188	26.27578986265936	29.340395393237667	22.444293896655786
34	22.57806244995997	26.486173554228	28.955348894500215	21.980415101311817
35	22.734987990392312	27.325244811233603	27.559770893637985	22.379996304736096
36	22.618464001970807	26.590872698158524	27.860565375377227	22.93009792449344
37	22.865553981646855	26.390835745519492	28.548869865122867	22.194740407710782
38	22.54505142575599	26.26741393114492	27.837654739175953	23.34987990392314
39	22.135616185255895	26.50095460984172	28.644207673831374	22.71922153107101
40	22.627086284412144	27.45925971546468	28.10568454763811	21.807969452485064
41	22.546283180390468	26.402906940937363	28.178604421999136	22.87220545667303
42	21.49165486235142	28.383322042249183	27.874853729137154	22.25016936626224
43	23.21266243764242	26.067130627578987	27.589825706719225	23.13038122805937
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	43.0
2	52.0
3	207.5
4	363.0
5	363.0
6	481.5
7	600.0
8	545.5
9	491.0
10	665.5
11	840.0
12	840.0
13	1299.5
14	1759.0
15	3084.5
16	4410.0
17	5039.0
18	5668.0
19	5668.0
20	5273.0
21	4878.0
22	4985.5
23	5093.0
24	6209.0
25	7325.0
26	7325.0
27	8305.5
28	9286.0
29	10541.0
30	11796.0
31	13268.0
32	14740.0
33	14740.0
34	18477.0
35	22214.0
36	23428.5
37	24643.0
38	25267.5
39	25892.0
40	25892.0
41	26231.0
42	26570.0
43	27759.0
44	28948.0
45	28732.0
46	28516.0
47	28516.0
48	27663.5
49	26811.0
50	27696.5
51	28582.0
52	29441.0
53	30300.0
54	30300.0
55	29072.5
56	27845.0
57	23599.5
58	19354.0
59	17464.5
60	15575.0
61	15575.0
62	13937.0
63	12299.0
64	9250.5
65	6202.0
66	5310.5
67	4419.0
68	4419.0
69	3790.0
70	3161.0
71	3258.5
72	3356.0
73	3044.5
74	2733.0
75	2733.0
76	1622.0
77	511.0
78	378.5
79	246.0
80	197.5
81	149.0
82	149.0
83	124.0
84	99.0
85	92.0
86	85.0
87	66.0
88	47.0
89	47.0
90	34.5
91	22.0
92	16.0
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	405925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.34155903774642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.40895754495347	48.04280585894706
2	6.139575553883528	6.67268214856133
3	1.6657927170967357	2.715653198422809
4	0.8167479738829765	1.7753343296688615
5	0.5078633088071801	1.3799041989325311
6	0.3421277698540892	1.1155053842387106
7	0.26430695526461795	1.0053996409519468
8	0.20431024068729214	0.8882029605059735
9	0.15577044676938223	0.761832803650907
>10	1.197331355185027	13.18425182633577
>50	0.1652226332832905	6.237017021100252
>100	0.12414773283017116	12.145994383845974
>500	0.006461220295994039	2.2656806372840084
>1k	0.0013845472062844369	1.8097356075538413
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3431	0.8452300301779885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2401	0.5914885754757652	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1384	0.3409496828231816	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	945	0.23280162591611753	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	939	0.23132352035474535	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	710	0.17490915809570734	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	664	0.16357701545852066	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	648	0.15963540062819487	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	639	0.1574182422861366	No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	633	0.15594013672476445	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	600	0.14781055613721747	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	593	0.14608609964894992	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	545	0.13426125515797252	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	538	0.132536798669705	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	534	0.13155139496212356	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	532	0.13105869310833282	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	514	0.1266243764242163	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	475	0.11701669027529715	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	466	0.1147995319332389	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	434	0.1069163022725873	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	420	0.10346738929605222	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	417	0.10272833651536614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.4635092689536244E-4	0.0	0.0
2	0.0	0.0	4.927018537907249E-4	0.0	0.0
3	0.0	0.0	4.927018537907249E-4	0.0	0.0
4	0.0	0.0	4.927018537907249E-4	2.4635092689536244E-4	0.0
5	0.0	0.0	4.927018537907249E-4	2.4635092689536244E-4	0.0
6	0.0	0.0	7.390527806860873E-4	2.4635092689536244E-4	0.0
7	0.0	0.0	0.0017244564882675372	4.927018537907249E-4	0.0
8	0.0	0.0	0.0019708074151628995	4.927018537907249E-4	0.0
9	0.0	0.0	0.0024635092689536246	0.0012317546344768123	0.0
10	0.0	0.0	0.0029562111227443492	0.0012317546344768123	0.0
11	0.0	0.0	0.004927018537907249	0.0012317546344768123	0.0
12	0.0	0.0	0.006158773172384061	0.0012317546344768123	0.0
13	0.0	0.0	0.006897825953070149	0.0012317546344768123	0.0
14	0.0	0.0	0.0071441768799655105	0.0012317546344768123	0.0
15	0.0	0.0	0.007636878733756236	0.0014781055613721746	0.0
16	0.0	0.0	0.008375931514442323	0.0014781055613721746	0.0
17	0.0	0.0	0.008622282441337685	0.0014781055613721746	0.0
18	0.0	0.0	0.008622282441337685	0.0017244564882675372	0.0
19	0.0	0.0	0.008868633368233047	0.0017244564882675372	0.0
20	0.0	0.0	0.008868633368233047	0.0017244564882675372	0.0
21	0.0	0.0	0.008868633368233047	0.002709860195848987	0.0
22	0.0	0.0	0.009361335222023773	0.0032025620496397116	0.0
23	0.0	0.0	0.009361335222023773	0.0036952639034304366	0.0
24	0.0	0.0	0.009607686148919135	0.004680667611011886	0.0
25	0.0	0.0	0.009607686148919135	0.005173369464802611	0.0
26	0.0	0.0	0.009607686148919135	0.005419720391697974	0.0
27	0.0	0.0	0.009607686148919135	0.00812958058754696	0.0
28	0.0	0.0	0.009607686148919135	0.02315698712816407	0.0
29	0.0	0.0	0.009607686148919135	0.046806676110118864	0.0
30	0.0	0.0	0.009607686148919135	0.0840056660713186	0.0
31	0.0	0.0	0.009607686148919135	0.15150582004064791	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	20	0.0018405336	37.0	25
GCTCGGT	20	0.0018405336	37.0	26
TCGGTAG	20	0.0018405336	37.0	28
CTATCGC	25	0.005492207	29.6	30
TTGTCGG	25	0.005492207	29.6	22
GCCGCTC	80	3.274181E-11	27.750002	27
GGTATCA	460	0.0	27.347826	1
CGAGTCG	55	6.244991E-7	26.90909	21
TCCGCCG	50	9.072182E-6	25.900002	31
GAGTCGC	60	1.3338486E-6	24.666666	22
CGCCTGG	60	1.3338486E-6	24.666666	36
GTATCAA	1395	0.0	24.666666	1
GGACCGT	45	1.3211531E-4	24.666666	6
CGCCGCC	60	1.3338486E-6	24.666666	33
TGTCCGG	60	1.3338486E-6	24.666666	14
GTCGCCG	65	2.6757534E-6	22.76923	24
CCGCTCT	90	3.810783E-9	22.61111	28
TTCGCCG	110	7.4578566E-11	21.863636	24
TGCTCGC	120	1.0913936E-11	21.583332	10
GCCGTCC	60	3.718648E-5	21.583332	27
>>END_MODULE