FastQCFastQC Report
Fri 10 Feb 2017
ERR1633206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466176
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17590.3773253020318506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.25591193025809994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6490.13921780615046678No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4930.10575404997254256No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4910.10532502745744096No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4800.10296540362438221No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4690.10060577979132344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA250.00549303429.59999818
GGTATCA2900.024.2413791
GTATCAA7550.023.2781471
TCACCGT500.00702876418.4999986
ACACCGT500.00702876418.4999986
TAGGACC1258.540155E-917.764
GTTTAGG1301.3867975E-817.0769211
ACTATCG1005.8627265E-616.6511
TATCGGT1005.8627265E-616.6513
CATATAC803.377173E-416.18753
TAGGCAG803.377173E-416.18755
TCAACGC10900.015.9541294
CTATCGG1059.319207E-615.85714412
ATAGAGT700.002589394615.8571436
TATACCT2700.015.7592595
ATCAACG11000.015.6409093
CCTATAC2250.015.6222223
CAACGCA11150.015.5964145
ATAATCC957.045294E-515.5789483
TAGTACA957.045294E-515.5789484
Produced by FastQC (version 0.11.5)