##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633206.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466176
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87438435269083	33.0	33.0	33.0	33.0	33.0
2	31.984317081960462	33.0	33.0	33.0	33.0	33.0
3	31.698815468835804	33.0	33.0	33.0	27.0	33.0
4	31.99509627265239	33.0	33.0	33.0	33.0	33.0
5	32.09121447693575	33.0	33.0	33.0	33.0	33.0
6	35.26649806081823	37.0	37.0	37.0	33.0	37.0
7	35.39934917284459	37.0	37.0	37.0	33.0	37.0
8	35.52760545373421	37.0	37.0	37.0	33.0	37.0
9	35.65177100494234	37.0	37.0	37.0	33.0	37.0
10	35.656854921746294	37.0	37.0	37.0	33.0	37.0
11	35.6756675590335	37.0	37.0	37.0	33.0	37.0
12	35.67193506315211	37.0	37.0	37.0	33.0	37.0
13	35.667097834294346	37.0	37.0	37.0	33.0	37.0
14	35.649417816447006	37.0	37.0	37.0	33.0	37.0
15	35.65703082097748	37.0	37.0	37.0	33.0	37.0
16	35.643224876441515	37.0	37.0	37.0	33.0	37.0
17	35.63877162273476	37.0	37.0	37.0	33.0	37.0
18	35.61389475219659	37.0	37.0	37.0	33.0	37.0
19	35.62577438563976	37.0	37.0	37.0	33.0	37.0
20	35.61224087040088	37.0	37.0	37.0	33.0	37.0
21	35.61713816241076	37.0	37.0	37.0	33.0	37.0
22	35.519241659802304	37.0	37.0	37.0	33.0	37.0
23	35.58638153830313	37.0	37.0	37.0	33.0	37.0
24	35.55457809925865	37.0	37.0	37.0	33.0	37.0
25	35.5693750858045	37.0	37.0	37.0	33.0	37.0
26	35.46655340472268	37.0	37.0	37.0	33.0	37.0
27	35.49250497666117	37.0	37.0	37.0	33.0	37.0
28	35.50973881109281	37.0	37.0	37.0	33.0	37.0
29	35.530728737644154	37.0	37.0	37.0	33.0	37.0
30	35.522403555738606	37.0	37.0	37.0	33.0	37.0
31	35.51419206479956	37.0	37.0	37.0	33.0	37.0
32	35.50233173736958	37.0	37.0	37.0	33.0	37.0
33	35.50438031987919	37.0	37.0	37.0	33.0	37.0
34	35.47677486614498	37.0	37.0	37.0	33.0	37.0
35	35.42189859623833	37.0	37.0	37.0	33.0	37.0
36	35.44401899711697	37.0	37.0	37.0	33.0	37.0
37	35.453601214991764	37.0	37.0	37.0	33.0	37.0
38	35.43003929846238	37.0	37.0	37.0	33.0	37.0
39	35.33594179022515	37.0	37.0	37.0	33.0	37.0
40	35.113984846924765	37.0	37.0	37.0	33.0	37.0
41	35.30356131589786	37.0	37.0	37.0	33.0	37.0
42	35.34458444879187	37.0	37.0	37.0	33.0	37.0
43	35.029647600906095	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	12.0
17	18.0
18	14.0
19	18.0
20	33.0
21	77.0
22	176.0
23	449.0
24	980.0
25	1846.0
26	2946.0
27	4380.0
28	6165.0
29	8483.0
30	11281.0
31	14213.0
32	18423.0
33	24364.0
34	35104.0
35	72728.0
36	264455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.07842531576057	21.540362438220757	13.953742792421748	24.427469453596924
2	15.551208127402527	21.930772926963208	39.09231706479956	23.42570188083471
3	17.170124588138385	27.790147926963204	30.33875617792422	24.70097130697419
4	12.093501166941241	18.317545304777592	40.384532880285555	29.204420647995605
5	13.103849190005493	37.79066275398133	36.04239600494234	13.063092051070841
6	28.724558964854474	40.67712623558484	17.838112644151565	12.760202155409118
7	25.809565485996703	32.72626647446458	24.3408069055464	17.12336113399231
8	23.824478308621636	36.733765788028556	20.895112575507962	18.546643327841846
9	25.251192682591984	15.809050658978583	20.97705587589237	37.96270078253707
10	14.222096375617793	28.57933484349259	34.801233868753435	22.397334912136188
11	32.865269769357496	24.29790465403624	25.0274574409665	17.809368135639758
12	22.130911930258097	26.241376647446458	31.155400535420096	20.472310886875345
13	27.128380697419	22.961928542009886	26.6845569055464	23.225133855024712
14	21.230822693574957	21.497460186710597	28.500394700713894	28.77132241900055
15	23.07626304228446	29.607701812191106	24.770687465678197	22.545347679846238
16	22.35722130697419	29.169455313014826	25.81020901976936	22.663114360241625
17	21.690091295991213	28.37447659253158	27.150260845689182	22.78517126578803
18	22.102167421746294	28.404293657331138	28.336508099945085	21.157030820977486
19	23.679468698517297	27.284544892915978	28.08252677100494	20.95345963756178
20	22.926963207029104	27.50184479681494	28.17691172432729	21.394280271828666
21	22.152577567270733	27.828330930807248	28.25692442339374	21.76216707852828
22	21.924552100494235	27.577138248215267	28.12843218012081	22.369877471169687
23	21.999416529379463	27.597302306425043	28.463069741900053	21.94021142229544
24	22.175744783086216	28.14065932180121	27.3293777457441	22.354218149368478
25	22.231732221306974	27.7884318369028	28.06107564524986	21.918760296540363
26	22.185397789676003	27.98041941241076	28.161895936298738	21.6722868616145
27	22.17467222679846	27.79808484349259	27.61510674080176	22.412136188907194
28	21.895378569467326	27.51235584843493	28.484735378912685	22.107530203185064
29	23.1009318369028	27.479535626029655	27.63698688907194	21.782545647995608
30	22.651316241076334	27.31479098023064	28.097113536518396	21.936779242174627
31	22.3602244645799	27.505062465678197	28.0173153487095	22.1173977210324
32	21.58219213344316	27.485112918725974	28.293391337177376	22.63930361065349
33	21.503895524437123	27.614034184514004	28.759953322350356	22.122116968698517
34	22.378028898956618	27.223623695771554	28.2588550247117	22.13949238056013
35	22.16780786655684	27.94652663371774	27.929794755628773	21.95587074409665
36	23.01426928885228	27.537239154310818	27.51750411861614	21.930987438220757
37	22.3196818369028	27.112935886875345	27.86930258099945	22.698079695222408
38	22.31067236408567	27.64621087314662	27.71528349807798	22.32783326468973
39	21.994482770455793	27.498627127951675	28.04134060955519	22.465549492037344
40	22.180464030752333	28.105479475562877	28.064293314113126	21.649763179571664
41	21.95801585667216	27.233491213618894	28.210160969247667	22.598331960461284
42	21.981826606260295	28.045630834706202	27.920570771554093	22.051971787479406
43	22.378028898956618	26.97221650192202	28.109984211971444	22.539770387149918
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	27.0
2	33.0
3	117.0
4	201.0
5	201.0
6	263.0
7	325.0
8	276.5
9	228.0
10	321.0
11	414.0
12	414.0
13	676.0
14	938.0
15	1706.5
16	2475.0
17	3098.5
18	3722.0
19	3722.0
20	3856.0
21	3990.0
22	4799.5
23	5609.0
24	7004.5
25	8400.0
26	8400.0
27	10519.5
28	12639.0
29	14600.0
30	16561.0
31	19047.5
32	21534.0
33	21534.0
34	24788.5
35	28043.0
36	30155.0
37	32267.0
38	34048.0
39	35829.0
40	35829.0
41	36772.0
42	37715.0
43	37769.5
44	37824.0
45	36708.5
46	35593.0
47	35593.0
48	33742.0
49	31891.0
50	31262.5
51	30634.0
52	28813.5
53	26993.0
54	26993.0
55	25913.0
56	24833.0
57	21493.5
58	18154.0
59	16923.5
60	15693.0
61	15693.0
62	13747.5
63	11802.0
64	9780.0
65	7758.0
66	6526.0
67	5294.0
68	5294.0
69	4320.0
70	3346.0
71	2792.0
72	2238.0
73	1794.5
74	1351.0
75	1351.0
76	1030.5
77	710.0
78	573.0
79	436.0
80	359.5
81	283.0
82	283.0
83	226.0
84	169.0
85	151.0
86	133.0
87	97.5
88	62.0
89	62.0
90	44.0
91	26.0
92	17.5
93	9.0
94	4.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	466176.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.80465432870042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.70042221778618	61.030018895045565
2	6.807179280702398	9.3673123472444
3	1.7206744531763425	3.5517123288907158
4	0.781342085981128	2.150398885535889
5	0.4317986507061154	1.4854878450716762
6	0.2829241840915157	1.1679900412587805
7	0.19530521090496478	0.9406535267437024
8	0.1540466894896673	0.8479303376653764
9	0.11221220843604268	0.6948649991611797
>10	0.6993306333002209	9.357702904372688
>50	0.07388939909334238	3.4568591578057104
>100	0.03993171741675524	5.170036893644112
>500	3.1442297178547427E-4	0.1404031276246978
>1k	6.288459435709485E-4	0.6386287099354513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1759	0.3773253020318506	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1193	0.25591193025809994	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	649	0.13921780615046678	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	493	0.10575404997254256	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	491	0.10532502745744096	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	480	0.10296540362438221	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	469	0.10060577979132344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1451125755079628E-4
2	0.0	0.0	0.0	0.0	2.1451125755079628E-4
3	0.0	0.0	0.0	2.1451125755079628E-4	2.1451125755079628E-4
4	0.0	0.0	0.0	2.1451125755079628E-4	2.1451125755079628E-4
5	0.0	0.0	4.2902251510159255E-4	2.1451125755079628E-4	2.1451125755079628E-4
6	0.0	0.0	6.435337726523887E-4	2.1451125755079628E-4	2.1451125755079628E-4
7	0.0	0.0	0.0010725562877539814	2.1451125755079628E-4	2.1451125755079628E-4
8	0.0	0.0	0.0010725562877539814	2.1451125755079628E-4	2.1451125755079628E-4
9	0.0	0.0	0.0010725562877539814	6.435337726523887E-4	2.1451125755079628E-4
10	0.0	0.0	0.0012870675453047775	6.435337726523887E-4	2.1451125755079628E-4
11	0.0	0.0	0.002574135090609555	6.435337726523887E-4	2.1451125755079628E-4
12	0.0	0.0	0.003217668863261944	6.435337726523887E-4	2.1451125755079628E-4
13	0.0	0.0	0.0034321801208127404	6.435337726523887E-4	2.1451125755079628E-4
14	0.0	0.0	0.0034321801208127404	6.435337726523887E-4	2.1451125755079628E-4
15	0.0	0.0	0.004290225151015925	6.435337726523887E-4	2.1451125755079628E-4
16	0.0	0.0	0.005362781438769906	6.435337726523887E-4	2.1451125755079628E-4
17	0.0	0.0	0.005362781438769906	6.435337726523887E-4	2.1451125755079628E-4
18	0.0	0.0	0.006006315211422295	6.435337726523887E-4	2.1451125755079628E-4
19	0.0	0.0	0.006220826468973092	6.435337726523887E-4	2.1451125755079628E-4
20	0.0	0.0	0.006435337726523888	8.580450302031851E-4	2.1451125755079628E-4
21	0.0	0.0	0.006435337726523888	0.0010725562877539814	2.1451125755079628E-4
22	0.0	0.0	0.006435337726523888	0.0019306013179571663	2.1451125755079628E-4
23	0.0	0.0	0.006435337726523888	0.0021451125755079627	2.1451125755079628E-4
24	0.0	0.0	0.006435337726523888	0.0021451125755079627	2.1451125755079628E-4
25	0.0	0.0	0.0066498489840746845	0.0021451125755079627	2.1451125755079628E-4
26	0.0	0.0	0.0066498489840746845	0.002359623833058759	2.1451125755079628E-4
27	0.0	0.0	0.0066498489840746845	0.0030031576057111477	2.1451125755079628E-4
28	0.0	0.0	0.0066498489840746845	0.012441652937946183	2.1451125755079628E-4
29	0.0	0.0	0.0066498489840746845	0.025526839648544755	2.1451125755079628E-4
30	0.0	0.0	0.0066498489840746845	0.043331274025260845	2.1451125755079628E-4
31	0.0	0.0	0.0066498489840746845	0.0858045030203185	2.1451125755079628E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATA	25	0.005493034	29.599998	18
GGTATCA	290	0.0	24.241379	1
GTATCAA	755	0.0	23.278147	1
TCACCGT	50	0.007028764	18.499998	6
ACACCGT	50	0.007028764	18.499998	6
TAGGACC	125	8.540155E-9	17.76	4
GTTTAGG	130	1.3867975E-8	17.076921	1
ACTATCG	100	5.8627265E-6	16.65	11
TATCGGT	100	5.8627265E-6	16.65	13
CATATAC	80	3.377173E-4	16.1875	3
TAGGCAG	80	3.377173E-4	16.1875	5
TCAACGC	1090	0.0	15.954129	4
CTATCGG	105	9.319207E-6	15.857144	12
ATAGAGT	70	0.0025893946	15.857143	6
TATACCT	270	0.0	15.759259	5
ATCAACG	1100	0.0	15.640909	3
CCTATAC	225	0.0	15.622222	3
CAACGCA	1115	0.0	15.596414	5
ATAATCC	95	7.045294E-5	15.578948	3
TAGTACA	95	7.045294E-5	15.578948	4
>>END_MODULE