FastQCFastQC Report
Fri 10 Feb 2017
ERR1633205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477632
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28650.5998341819643576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.45746516146321853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10400.2177408548840949No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.13357563982312742No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5110.10698613158247354No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4780.10007704676403592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG250.00549316729.627
TGCGCCA400.001929543623.12500214
TTCGCCG1054.0017767E-1122.9047624
CCGCTCT1155.456968E-1222.5217428
CGCCGCT1250.022.19999926
GCTTCGC1201.0913936E-1121.58333422
GCCGCTC1301.8189894E-1221.34615327
GTATCAA12500.021.3120021
CCGGCAG1059.786163E-1021.14285716
AGCTTCG1059.786163E-1021.14285721
GCCGGCA1059.786163E-1021.14285715
GGTATCA5300.020.594341
TCGCCGG1202.382876E-1020.04166813
CGCTCTC1355.638867E-1119.18518629
TCGCCGC1306.9303496E-1018.525
GTACCAC500.00702900918.53
ATGGTAC701.2173051E-418.53
GCTCGCC1409.276846E-1118.511
TAATGCT609.226043E-418.54
CTCGCCG1451.546141E-1017.86206812
Produced by FastQC (version 0.11.5)