##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633205.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477632
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86784595671982	33.0	33.0	33.0	33.0	33.0
2	31.99494589977221	33.0	33.0	33.0	33.0	33.0
3	31.709167308723032	33.0	33.0	33.0	27.0	33.0
4	31.992642871499395	33.0	33.0	33.0	33.0	33.0
5	32.08538163272143	33.0	33.0	33.0	33.0	33.0
6	35.26044946737237	37.0	37.0	37.0	33.0	37.0
7	35.40340680691411	37.0	37.0	37.0	33.0	37.0
8	35.53834751440439	37.0	37.0	37.0	33.0	37.0
9	35.64581100093796	37.0	37.0	37.0	33.0	37.0
10	35.65258190406003	37.0	37.0	37.0	33.0	37.0
11	35.67460094801018	37.0	37.0	37.0	33.0	37.0
12	35.67586761356023	37.0	37.0	37.0	33.0	37.0
13	35.67111081334584	37.0	37.0	37.0	33.0	37.0
14	35.65409562173389	37.0	37.0	37.0	33.0	37.0
15	35.66216250167493	37.0	37.0	37.0	33.0	37.0
16	35.65720261624012	37.0	37.0	37.0	33.0	37.0
17	35.64648097279914	37.0	37.0	37.0	33.0	37.0
18	35.627545893072494	37.0	37.0	37.0	33.0	37.0
19	35.62559669368886	37.0	37.0	37.0	33.0	37.0
20	35.63017343896556	37.0	37.0	37.0	33.0	37.0
21	35.633232279244275	37.0	37.0	37.0	33.0	37.0
22	35.540707071552994	37.0	37.0	37.0	33.0	37.0
23	35.58176797199518	37.0	37.0	37.0	33.0	37.0
24	35.575888968913304	37.0	37.0	37.0	33.0	37.0
25	35.591051269596676	37.0	37.0	37.0	33.0	37.0
26	35.47780299477422	37.0	37.0	37.0	33.0	37.0
27	35.50619723971593	37.0	37.0	37.0	33.0	37.0
28	35.522303781991155	37.0	37.0	37.0	33.0	37.0
29	35.53462917057484	37.0	37.0	37.0	33.0	37.0
30	35.52158774956452	37.0	37.0	37.0	33.0	37.0
31	35.52243568270133	37.0	37.0	37.0	33.0	37.0
32	35.504359004421815	37.0	37.0	37.0	33.0	37.0
33	35.49416705748359	37.0	37.0	37.0	33.0	37.0
34	35.46924410424762	37.0	37.0	37.0	33.0	37.0
35	35.406836225378534	37.0	37.0	37.0	33.0	37.0
36	35.42293020568136	37.0	37.0	37.0	33.0	37.0
37	35.42339499866006	37.0	37.0	37.0	33.0	37.0
38	35.409756883960874	37.0	37.0	37.0	33.0	37.0
39	35.302042157979365	37.0	37.0	37.0	33.0	37.0
40	35.092317935146724	37.0	37.0	37.0	33.0	37.0
41	35.23487119790969	37.0	37.0	37.0	33.0	37.0
42	35.27505276028407	37.0	37.0	37.0	33.0	37.0
43	34.94182341216669	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	21.0
16	24.0
17	18.0
18	13.0
19	11.0
20	28.0
21	84.0
22	212.0
23	475.0
24	975.0
25	1764.0
26	3036.0
27	4503.0
28	6382.0
29	8733.0
30	11659.0
31	14877.0
32	18930.0
33	24976.0
34	35884.0
35	73498.0
36	271527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.92879036580464	21.119187994104248	13.807073227924427	24.144948412166688
2	15.576636406270936	21.96125887712716	39.1221693688865	23.339935347715397
3	17.470353745142702	27.984934007771674	30.43054066729197	24.11417157979365
4	12.057609205413373	18.137185113225243	40.2598653356559	29.54534034570548
5	13.351701728527402	37.54920105855554	35.56126892670508	13.537828286211978
6	28.589374246281658	40.731986131582474	17.680138684175265	12.998500937960605
7	25.714985595604983	32.78988844968511	24.21592355621064	17.27920239849926
8	24.213620527937827	36.02731810263969	21.023717003885835	18.735344365536648
9	25.639404395015408	15.56637746214659	20.938923690205012	37.85529445263299
10	14.60915516548305	28.494950087096342	34.18761724507571	22.708277502344902
11	33.02081937558622	24.225554401715126	24.74394512930457	18.00968109339408
12	22.476299745410692	26.368208160257268	30.744589977220954	20.410902117111082
13	27.345320246549644	22.914503215864933	26.713871767385772	23.02630477019965
14	21.549016816293715	21.435121599892803	28.695732279244275	28.320129304569207
15	23.21850294787619	29.303313010853543	25.276572758944127	22.20161128232614
16	22.203495578185716	29.112789762829962	26.049134061369422	22.6345805976149
17	21.685314216802894	28.00482379740051	27.13427911027737	23.17558287551923
18	22.270702130510518	27.841518156237438	28.62517586761356	21.262603845638484
19	23.832364665684043	27.053045022109075	28.404503885836796	20.710086426370093
20	23.067759279110277	26.78777803832239	28.65323093930055	21.491231743266784
21	22.12791437759614	27.515744338737775	28.78555038188396	21.570790901782125
22	22.040818035642502	27.25780517218277	28.49599691812944	22.20537987404529
23	21.809887109741393	27.18368953503953	29.158431595872976	21.847991759346108
24	22.22087297333512	27.858686185180222	27.700824065389252	22.219616776095403
25	22.158063111349325	27.379028205815352	28.699710237170038	21.76319844566528
26	22.290382553932737	27.643667090982177	28.48427241055876	21.58167794452633
27	22.315087766313816	27.513860042878196	28.048790700790565	22.12226149001742
28	21.788950489079458	27.302190807986065	28.771732212247088	22.137126490687393
29	22.98380343025593	27.253827214257004	27.937617245075707	21.824752110411364
30	22.574911228728393	27.04341417660458	28.530542342221626	21.851132252445396
31	22.213335789896824	27.277485595604983	28.59984255661262	21.90933605788557
32	21.469248291571756	27.061000937960607	28.86804066729197	22.60171010317567
33	21.4123006833713	27.24566193219885	29.122420608334448	22.219616776095403
34	22.268608468444327	27.067281924159186	28.739699182634332	21.92441042476216
35	21.934669368886507	27.670047233016216	28.413925365134663	21.981358032962618
36	22.652586091384162	27.468636942248427	28.01089541739247	21.867881548974943
37	22.17774353477154	27.088218544821117	28.280559091518153	22.453478828889185
38	22.243693889856626	27.355369824467374	28.048371968377328	22.352564317298672
39	21.696410625753717	27.37232848720354	28.426068605118587	22.50519228192416
40	22.052542543213185	27.811160056277632	28.5822557952566	21.55404160525258
41	21.906614297199518	26.97264839876725	28.407016280316228	22.713721023717003
42	21.54503885836795	27.92840513198446	28.385451561034436	22.14110444861316
43	22.332883893876456	26.887645718879803	28.20937458126759	22.57009580597615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	38.0
2	58.0
3	198.5
4	339.0
5	339.0
6	406.5
7	474.0
8	460.0
9	446.0
10	611.5
11	777.0
12	777.0
13	1245.5
14	1714.0
15	2944.5
16	4175.0
17	4712.0
18	5249.0
19	5249.0
20	4980.5
21	4712.0
22	5077.0
23	5442.0
24	6846.0
25	8250.0
26	8250.0
27	10231.0
28	12212.0
29	14057.0
30	15902.0
31	18289.0
32	20676.0
33	20676.0
34	24331.5
35	27987.0
36	29623.5
37	31260.0
38	33033.5
39	34807.0
40	34807.0
41	35930.0
42	37053.0
43	37203.0
44	37353.0
45	36702.5
46	36052.0
47	36052.0
48	34772.5
49	33493.0
50	33295.0
51	33097.0
52	31407.5
53	29718.0
54	29718.0
55	27933.0
56	26148.0
57	22716.0
58	19284.0
59	17737.0
60	16190.0
61	16190.0
62	14079.0
63	11968.0
64	9996.0
65	8024.0
66	6756.0
67	5488.0
68	5488.0
69	4583.5
70	3679.0
71	2994.0
72	2309.0
73	1867.5
74	1426.0
75	1426.0
76	1102.0
77	778.0
78	601.0
79	424.0
80	351.0
81	278.0
82	278.0
83	217.0
84	156.0
85	139.0
86	122.0
87	90.5
88	59.0
89	59.0
90	45.5
91	32.0
92	17.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	477632.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.99316018630718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.69822081379026	60.308723360324855
2	6.735263070278559	9.159036416687382
3	1.7026407710988443	3.4730378006718388
4	0.7951503254575516	2.1625913380411834
5	0.4648548739715213	1.5803475954665642
6	0.2849739181214461	1.1625766358250644
7	0.21767394337957055	1.0360237510414612
8	0.16100704069847124	0.8757902007467547
9	0.11297054112962271	0.6913101689424215
>10	0.711488989505264	9.324711142803672
>50	0.07125822109372698	3.483796921931127
>100	0.042941635129881424	5.210461943669107
>500	6.223425381142233E-4	0.24309988115790807
>1k	9.335138071713348E-4	1.28849284269074
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2865	0.5998341819643576	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2185	0.45746516146321853	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1040	0.2177408548840949	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	638	0.13357563982312742	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	511	0.10698613158247354	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	478	0.10007704676403592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.09366206619322E-4	0.0	0.0
4	0.0	0.0	4.18732413238644E-4	0.0	0.0
5	0.0	0.0	8.37464826477288E-4	0.0	0.0
6	0.0	0.0	0.0010468310330966099	2.09366206619322E-4	0.0
7	0.0	0.0	0.002512394479431864	2.09366206619322E-4	0.0
8	2.09366206619322E-4	0.0	0.002931126892670508	2.09366206619322E-4	0.0
9	2.09366206619322E-4	0.0	0.0035592255125284737	0.001674929652954576	0.0
10	6.28098619857966E-4	0.0	0.0035592255125284737	0.001884295859573898	0.0
11	6.28098619857966E-4	0.0	0.006280986198579659	0.001884295859573898	0.0
12	6.28098619857966E-4	0.0	0.007955915851534236	0.002303028272812542	0.0
13	6.28098619857966E-4	0.0	0.009002746884630845	0.002303028272812542	0.0
14	6.28098619857966E-4	0.0	0.00963084550448881	0.002303028272812542	0.0
15	8.37464826477288E-4	0.0	0.0104683103309661	0.002303028272812542	0.0
16	8.37464826477288E-4	0.0	0.010887042744204744	0.002303028272812542	0.0
17	8.37464826477288E-4	0.0	0.011305775157443387	0.002512394479431864	0.0
18	8.37464826477288E-4	0.0	0.01151514136406271	0.002721760686051186	0.0
19	8.37464826477288E-4	0.0	0.01151514136406271	0.002931126892670508	0.0
20	8.37464826477288E-4	0.0	0.012143239983920676	0.003349859305909152	0.0
21	8.37464826477288E-4	0.0	0.013608803430255929	0.003349859305909152	0.0
22	8.37464826477288E-4	0.0	0.014027535843494573	0.004396690339005762	0.0
23	8.37464826477288E-4	0.0	0.014027535843494573	0.005862253785341016	0.0
24	8.37464826477288E-4	0.0	0.014027535843494573	0.006280986198579659	0.0
25	8.37464826477288E-4	0.0	0.014236902050113895	0.006699718611818304	0.0
26	8.37464826477288E-4	0.0	0.014236902050113895	0.006699718611818304	0.0
27	8.37464826477288E-4	0.0	0.014236902050113895	0.008793380678011524	0.0
28	8.37464826477288E-4	0.0	0.014236902050113895	0.022402184108267453	0.0
29	8.37464826477288E-4	0.0	0.014236902050113895	0.04501373442315423	0.0
30	8.37464826477288E-4	0.0	0.014236902050113895	0.07893105989548439	0.0
31	8.37464826477288E-4	0.0	0.014236902050113895	0.14320648532761623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGG	25	0.005493167	29.6	27
TGCGCCA	40	0.0019295436	23.125002	14
TTCGCCG	105	4.0017767E-11	22.90476	24
CCGCTCT	115	5.456968E-12	22.52174	28
CGCCGCT	125	0.0	22.199999	26
GCTTCGC	120	1.0913936E-11	21.583334	22
GCCGCTC	130	1.8189894E-12	21.346153	27
GTATCAA	1250	0.0	21.312002	1
CCGGCAG	105	9.786163E-10	21.142857	16
AGCTTCG	105	9.786163E-10	21.142857	21
GCCGGCA	105	9.786163E-10	21.142857	15
GGTATCA	530	0.0	20.59434	1
TCGCCGG	120	2.382876E-10	20.041668	13
CGCTCTC	135	5.638867E-11	19.185186	29
TCGCCGC	130	6.9303496E-10	18.5	25
GTACCAC	50	0.007029009	18.5	3
ATGGTAC	70	1.2173051E-4	18.5	3
GCTCGCC	140	9.276846E-11	18.5	11
TAATGCT	60	9.226043E-4	18.5	4
CTCGCCG	145	1.546141E-10	17.862068	12
>>END_MODULE