FastQCFastQC Report
Fri 10 Feb 2017
ERR1633204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234151
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82453.5212320254878264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64212.7422475240336364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31611.3499835576188015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.6598306221199141No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11580.49455266046269286No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11560.49369851079004573No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9430.40273157065312554No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9290.39675252294459556No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8950.38223197850959423No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.37497170629209353No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7170.30621265764399896No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6940.2963899364085569No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6590.28144231713723195No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6440.27503619459237844No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6380.27247374557443704No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5710.24385973154075788No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5410.23104748645105083No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5270.22506843874252083No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5250.22421428906987373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.22421428906987373No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.21823524136134376No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.2143915678344316No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4880.20841252012590164No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4770.2037146969263424No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4770.2037146969263424No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4700.2007251730720774No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4640.19816272405413599No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4470.19090245183663535No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4360.18620462863707607No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4330.18492340412810537No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4320.1844963292917818No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4290.1832151047828111No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4230.1806526557648697No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4140.17680898223795755No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4120.17595483256531042No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4040.1725382338747219No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3960.1691216351841333No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3930.16784041067516262No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA3900.1665591861661919No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3870.1652779616572212No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3870.1652779616572212No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3790.16186136296663264No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3740.1597259887850148No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3690.15759061460339696No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3650.15588231525810267No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3490.14904911787692557No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3390.1447783695136899No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3360.14349714500471916No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3340.14264299533207203No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3320.1417888456594249No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3280.14008054631413064No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3260.13922639664148348No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3250.13879932180515991No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.13367442376927707No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3130.13367442376927707No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3100.13239319926030638No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3040.12983075024236496No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3040.12983075024236496No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3000.1281224508970707No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC2930.1251329270428057No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2900.12385170253383501No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2890.12342462769751143No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2880.12299755286118785No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2870.1225704780248643No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2830.12086217867957003No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2810.1200080290069229No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG2800.11958095417059932No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2740.11701850515265791No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2730.11659143031633433No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2730.11659143031633433No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2710.1157372806436872No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.11445605613471649No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2680.11445605613471649No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2670.11402898129839291No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2660.11360190646206934No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2650.11317483162574579No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2610.1114665322804515No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2570.10975823293515723No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2570.10975823293515723No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA2530.10804993358986296No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG2500.10676870908089225No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2470.10548748457192153No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2450.1046333348992744No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG2430.10377918522662727No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2410.10292503555398011No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2390.10207088588133298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAAC200.00183894537.02
GGGATCT200.00183894537.018
CGGGATC401.5928745E-632.37517
GGGAGTC250.005487520229.59999819
CCGCTCT2400.026.97916828
GCCGCTC2400.026.97916827
CGCTCTC2400.026.20833429
GCCGGCA2400.026.20833415
GCTCTCT2400.026.20833430
TGGCCAT509.050786E-625.89999832
GGCAGCT2450.025.67346818
CCGGCAG2450.025.67346816
GCAGCTT2500.025.1619
CGCCGGC2500.025.1614
CAGCTTC2500.025.1620
TTCGCCG2550.024.66666624
CTAGTGC451.3188209E-424.6666669
ACTAGTG901.4188117E-1024.6666668
CTCTCTT2600.024.19230831
ACAGCCT551.894283E-523.54545426
Produced by FastQC (version 0.11.5)