FastQCFastQC Report
Fri 10 Feb 2017
ERR1633203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463330
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19770.42669371722098726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.3351822674983273No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10750.23201605766947964No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9710.20956985302052533No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9530.20568493298512938No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8320.1795696371916345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7600.16402995705005072No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7530.1625191548140634No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7260.1566917747609695No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7110.15345434139813957No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6970.15043273692616493No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6760.145900330218203No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6510.14050460794681977No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6510.14050460794681977No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6410.13834631903826647No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6340.13683551680227915No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6220.13424557011201518No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT5980.12906567673148728No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5920.1277707033863553No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5670.12237498111497204No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT5660.12215915222411672No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5640.12172749444240606No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5420.11697925884358881No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5390.11633177217102281No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG5010.10813027431852028No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4980.1074827876459543No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG4930.10640364319167764No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4880.10532449873740099No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4700.10143957870200504No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4670.10079209202943906No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG4640.10014460535687306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTT754.6020432E-1027.13333113
TTAACGA358.861373E-426.4285735
AATACGC358.861373E-426.428575
CGAGTTC809.749783E-1025.43750214
GCCGAGT851.9681465E-923.94117712
GGTATCA3100.023.8709681
GTTTCGG701.9177787E-723.78571321
TTTCGGG701.9177787E-723.78571322
TGCGGTA551.8994822E-523.54545436
ATACGCC400.001929456723.1250026
GTATCAA7850.022.6242051
GGGGCGA502.69873E-422.19999930
TATTAGA2800.021.803572
GTGACAC2550.021.76470624
GCACCGC2650.021.6415110
CACCGCC2650.021.6415111
TGCCGAG1059.786163E-1021.14285711
ATTAGAG2650.020.9433963
CTCGGTA450.00382241420.55555527
GACACAT2700.020.55555326
Produced by FastQC (version 0.11.5)