##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633203.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	463330
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.894712192174044	33.0	33.0	33.0	33.0	33.0
2	31.994994928021065	33.0	33.0	33.0	33.0	33.0
3	31.70732739084454	33.0	33.0	33.0	27.0	33.0
4	31.988861070942956	33.0	33.0	33.0	33.0	33.0
5	32.1003021604472	33.0	33.0	33.0	33.0	33.0
6	35.28596680551659	37.0	37.0	37.0	33.0	37.0
7	35.43037359981007	37.0	37.0	37.0	33.0	37.0
8	35.54356290333024	37.0	37.0	37.0	33.0	37.0
9	35.66304361901884	37.0	37.0	37.0	33.0	37.0
10	35.665350829862085	37.0	37.0	37.0	33.0	37.0
11	35.683387650270866	37.0	37.0	37.0	33.0	37.0
12	35.69244814710897	37.0	37.0	37.0	33.0	37.0
13	35.68982582608508	37.0	37.0	37.0	33.0	37.0
14	35.674350894610754	37.0	37.0	37.0	33.0	37.0
15	35.66936093065418	37.0	37.0	37.0	33.0	37.0
16	35.6662119871366	37.0	37.0	37.0	33.0	37.0
17	35.652791746703215	37.0	37.0	37.0	33.0	37.0
18	35.639785897740275	37.0	37.0	37.0	33.0	37.0
19	35.6312908725962	37.0	37.0	37.0	33.0	37.0
20	35.63143763624199	37.0	37.0	37.0	33.0	37.0
21	35.63188008546824	37.0	37.0	37.0	33.0	37.0
22	35.54166792566853	37.0	37.0	37.0	33.0	37.0
23	35.588651716918825	37.0	37.0	37.0	33.0	37.0
24	35.575870330002374	37.0	37.0	37.0	33.0	37.0
25	35.5870567414154	37.0	37.0	37.0	33.0	37.0
26	35.48536248462219	37.0	37.0	37.0	33.0	37.0
27	35.50887488399197	37.0	37.0	37.0	33.0	37.0
28	35.53139878704163	37.0	37.0	37.0	33.0	37.0
29	35.541436988755315	37.0	37.0	37.0	33.0	37.0
30	35.539809638918264	37.0	37.0	37.0	33.0	37.0
31	35.51336628321067	37.0	37.0	37.0	33.0	37.0
32	35.5110763386787	37.0	37.0	37.0	33.0	37.0
33	35.51305333131893	37.0	37.0	37.0	33.0	37.0
34	35.4908143224052	37.0	37.0	37.0	33.0	37.0
35	35.42821746919043	37.0	37.0	37.0	33.0	37.0
36	35.45879826473572	37.0	37.0	37.0	33.0	37.0
37	35.45284138734811	37.0	37.0	37.0	33.0	37.0
38	35.43707077029331	37.0	37.0	37.0	33.0	37.0
39	35.336764293268295	37.0	37.0	37.0	33.0	37.0
40	35.128100921589365	37.0	37.0	37.0	33.0	37.0
41	35.2985863207649	37.0	37.0	37.0	33.0	37.0
42	35.34213843265059	37.0	37.0	37.0	33.0	37.0
43	35.02202102173397	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	15.0
17	16.0
18	17.0
19	14.0
20	25.0
21	71.0
22	192.0
23	452.0
24	920.0
25	1740.0
26	2862.0
27	4344.0
28	6057.0
29	8235.0
30	10925.0
31	14208.0
32	18296.0
33	24132.0
34	34691.0
35	72586.0
36	263524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90611443247793	21.88461787494874	13.850819070640796	24.35844862193253
2	15.709321649796044	21.76871776055943	38.946755012625985	23.57520557701854
3	16.607601493535924	28.48423369952302	30.143094554637084	24.76507025230397
4	11.676990481945912	18.41538428334017	41.092310016618825	28.815315218095094
5	12.828653443549953	38.071137202425916	36.40666479614961	12.693544557874517
6	28.051496773358082	41.695551766559475	17.597608615889325	12.655342844193123
7	25.785725077158826	32.76001985625796	24.51017633220383	16.944078734379385
8	23.666932855632055	36.350765113418085	20.938855675220687	19.043446355729177
9	25.289534457082425	15.50557917682861	20.97274081108497	38.232145555003996
10	14.67334297369046	28.087756027021776	34.82787645954287	22.41102453974489
11	32.738005309390715	24.29477909913021	24.75881121446917	18.208404377009906
12	22.206418751214038	26.979906330261365	30.235037662141455	20.57863725638314
13	26.499039561435694	23.24477154511903	26.660047914013767	23.596140979431507
14	21.153605421621737	21.488356031338355	28.54315498672652	28.81488356031338
15	23.472255196080546	29.466039324023914	25.440614680681158	21.621090799214382
16	22.46541342024043	28.996827315304426	25.849826257742865	22.687933006712278
17	21.633177217102283	28.455528457039257	26.635443420456262	23.275850905402198
18	21.676342995273348	28.59128482938726	28.564306218030346	21.168065957309047
19	23.860099712947573	26.906524507370555	28.159195389894894	21.074180389786974
20	22.842898150346404	27.224224634709604	28.622148360779576	21.310728854164417
21	21.768070273886863	27.98221569939352	28.59257980273239	21.657134223987224
22	21.8079986186951	27.66645803207217	28.100274102691387	22.425269246541344
23	21.74972481816416	27.6938683012108	28.94157511924546	21.614831761379577
24	22.114907301491378	28.621285045216155	26.638680853819093	22.625126799473378
25	22.07001489219347	27.808257613364123	28.390779789782663	21.730947704659744
26	22.043467938618264	28.18703731681523	28.425096583428655	21.34439816113785
27	22.053180238706755	28.023654846437747	27.223577148037037	22.699587766818468
28	21.359937841279432	27.64271685407809	29.020568493298516	21.976776811343967
29	23.751321951956488	27.386743789523667	27.32652752897503	21.535406729544817
30	22.85649537047029	26.9304815142555	28.26840480866769	21.94461830660652
31	21.99469060928496	27.721710228131137	28.293656788897763	21.98994237368614
32	21.089504241037705	27.335592342390953	28.482507068396174	23.092396348175168
33	20.996050331297347	27.36106015151188	29.255606155439967	22.387283361750804
34	22.361383894848164	27.028467830703818	28.891286987676168	21.718861286771848
35	22.06073424988669	28.210994323700174	27.636241987352427	22.092029439060713
36	23.19038266462349	27.792933762113396	27.334081540154965	21.682602033108154
37	21.904689961798287	26.99889927265664	27.956100403600026	23.140310361945048
38	22.13886430837632	27.66753717652645	27.81537996676235	22.37821854833488
39	21.49418341139145	27.54516219541148	28.47149979496255	22.48915459823452
40	21.74281829365679	28.48056460837848	28.443657868042216	21.33295922992252
41	21.674184706364795	27.062568795458958	28.3195562558004	22.943690242375844
42	21.33382254548594	28.39164310534608	28.094230893747437	22.180303455420542
43	22.552824121036842	26.728249843524054	27.909049705393564	22.80987633004554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	27.0
2	42.0
3	131.0
4	220.0
5	220.0
6	273.5
7	327.0
8	299.5
9	272.0
10	404.5
11	537.0
12	537.0
13	840.5
14	1144.0
15	2034.0
16	2924.0
17	3361.5
18	3799.0
19	3799.0
20	3685.0
21	3571.0
22	3991.5
23	4412.0
24	5604.5
25	6797.0
26	6797.0
27	8731.0
28	10665.0
29	12425.0
30	14185.0
31	16791.0
32	19397.0
33	19397.0
34	23969.5
35	28542.0
36	31093.5
37	33645.0
38	35700.5
39	37756.0
40	37756.0
41	39314.0
42	40872.0
43	41444.5
44	42017.0
45	39765.5
46	37514.0
47	37514.0
48	34894.5
49	32275.0
50	31923.0
51	31571.0
52	29093.0
53	26615.0
54	26615.0
55	25971.5
56	25328.0
57	20953.0
58	16578.0
59	15416.0
60	14254.0
61	14254.0
62	12441.0
63	10628.0
64	8526.0
65	6424.0
66	5335.5
67	4247.0
68	4247.0
69	3438.5
70	2630.0
71	2214.0
72	1798.0
73	1402.0
74	1006.0
75	1006.0
76	778.0
77	550.0
78	424.5
79	299.0
80	247.0
81	195.0
82	195.0
83	155.0
84	115.0
85	101.5
86	88.0
87	70.5
88	53.0
89	53.0
90	38.0
91	23.0
92	11.5
93	0.0
94	1.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	463330.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.64787362094455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.28626933137174	47.700090152645316
2	6.9548641471176005	7.601370739250419
3	1.886962930953116	3.0935553533439903
4	0.8762106640435705	1.915321985359078
5	0.5621803737842629	1.5360981009368886
6	0.37057398743711206	1.2150648259603678
7	0.27023218322021947	1.033732993785134
8	0.20154314050183586	0.8811123257050077
9	0.16908877538295214	0.8316307825063065
>10	1.1429290950864774	12.244349168358578
>50	0.15503613416673923	5.926945180907555
>100	0.11406803653516615	11.773117268795783
>500	0.008836256351315687	3.2368500011732624
>1k	0.0012049440479066846	1.0107611212723178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1977	0.42669371722098726	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1553	0.3351822674983273	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1075	0.23201605766947964	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	971	0.20956985302052533	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	953	0.20568493298512938	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	832	0.1795696371916345	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	760	0.16402995705005072	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	753	0.1625191548140634	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	726	0.1566917747609695	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	711	0.15345434139813957	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	697	0.15043273692616493	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	676	0.145900330218203	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	651	0.14050460794681977	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	651	0.14050460794681977	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	641	0.13834631903826647	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	634	0.13683551680227915	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	622	0.13424557011201518	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	598	0.12906567673148728	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	592	0.1277707033863553	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	567	0.12237498111497204	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	566	0.12215915222411672	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	564	0.12172749444240606	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	542	0.11697925884358881	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	539	0.11633177217102281	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	501	0.10813027431852028	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	498	0.1074827876459543	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	493	0.10640364319167764	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	488	0.10532449873740099	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	470	0.10143957870200504	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	467	0.10079209202943906	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	464	0.10014460535687306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	6.474866725659897E-4	0.0	0.0
7	0.0	0.0	0.001942460017697969	0.0	0.0
8	0.0	0.0	0.002589946690263959	0.0	0.0
9	0.0	0.0	0.0032374333628299484	4.316577817106598E-4	0.0
10	0.0	0.0	0.0034532622536852784	4.316577817106598E-4	0.0
11	0.0	0.0	0.005179893380527918	4.316577817106598E-4	0.0
12	0.0	0.0	0.005611551162238577	4.316577817106598E-4	0.0
13	0.0	0.0	0.005827380053093907	4.316577817106598E-4	0.0
14	0.0	0.0	0.006474866725659897	6.474866725659897E-4	0.0
15	0.0	0.0	0.007122353398225887	8.633155634213196E-4	0.0
16	0.0	0.0	0.008633155634213196	8.633155634213196E-4	0.0
17	0.0	0.0	0.008633155634213196	0.0010791444542766495	0.0
18	0.0	0.0	0.008848984525068526	0.0015108022359873092	0.0
19	0.0	0.0	0.009064813415923856	0.0015108022359873092	0.0
20	0.0	0.0	0.009496471197634516	0.0017266311268426392	0.0
21	0.0	0.0	0.009496471197634516	0.001942460017697969	0.0
22	0.0	0.0	0.009496471197634516	0.002589946690263959	0.0
23	0.0	0.0	0.010359786761055836	0.0034532622536852784	0.0
24	0.0	0.0	0.010575615651911166	0.004100748926251268	0.0
25	0.0	0.0	0.010575615651911166	0.004100748926251268	0.0
26	0.0	0.0	0.010575615651911166	0.004748235598817258	0.0
27	0.0	0.0	0.010575615651911166	0.006690695616515227	0.0
28	0.0	0.0	0.010575615651911166	0.01726631126842639	0.0
29	0.0	0.0	0.010575615651911166	0.03647508255455075	0.0
30	0.0	0.0	0.010575615651911166	0.06474866725659897	0.0
31	0.0	0.0	0.010575615651911166	0.11978503442470809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAGTT	75	4.6020432E-10	27.133331	13
TTAACGA	35	8.861373E-4	26.42857	35
AATACGC	35	8.861373E-4	26.42857	5
CGAGTTC	80	9.749783E-10	25.437502	14
GCCGAGT	85	1.9681465E-9	23.941177	12
GGTATCA	310	0.0	23.870968	1
GTTTCGG	70	1.9177787E-7	23.785713	21
TTTCGGG	70	1.9177787E-7	23.785713	22
TGCGGTA	55	1.8994822E-5	23.545454	36
ATACGCC	40	0.0019294567	23.125002	6
GTATCAA	785	0.0	22.624205	1
GGGGCGA	50	2.69873E-4	22.199999	30
TATTAGA	280	0.0	21.80357	2
GTGACAC	255	0.0	21.764706	24
GCACCGC	265	0.0	21.64151	10
CACCGCC	265	0.0	21.64151	11
TGCCGAG	105	9.786163E-10	21.142857	11
ATTAGAG	265	0.0	20.943396	3
CTCGGTA	45	0.003822414	20.555555	27
GACACAT	270	0.0	20.555553	26
>>END_MODULE