FastQCFastQC Report
Fri 10 Feb 2017
ERR1633202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555538
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28320.5097761089250421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20400.36721160388668284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10400.18720591570693634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.10116319675701753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAGC1250.026.6417
CGAGTCG358.863602E-426.42857221
GGTATCA3450.026.2753621
GTATCAA11400.026.1271931
GCTTCGC1300.025.61538722
TTCGCCG1300.025.61538724
CTTCGCC1350.024.66666823
CGCTCTC1200.024.66666829
TGCTCGC1350.024.66666810
CCGCTCT1400.023.78571528
GCCGCTC1350.023.29629727
AGCTTCG1500.022.221
GCGCCTT502.6996672E-422.213
GCCGGCA1400.021.14285915
TCGCCGC1600.020.812525
CGCCGCT1600.020.812526
CTGCTCG1900.020.4473699
CCGGCAG1450.020.41379416
TCGCCGG1500.019.73333413
GGTAACG759.251644E-619.73333425
Produced by FastQC (version 0.11.5)