FastQCFastQC Report
Fri 10 Feb 2017
ERR1633201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577496
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35010.606237965284608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.4419078227381661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13020.22545610705528696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.12761993156662557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC251.2328652E-436.99999628
TTTGGCG303.5972794E-430.83333422
CCGGGTC250.00549410729.59999814
GCGCTCA358.864029E-426.4285731
GGTATCA4900.024.5408151
AATACCG551.9005338E-523.5454545
GTATCAA14300.023.2867131
TTGGCGT400.001930029623.12500223
GGCACCG1400.022.4642859
GCTCGGA1400.021.14285711
AGGACCG450.003823538320.5555555
GCCGGCA450.003823538320.55555515
CGCTCAT450.003823538320.55555532
CGAGCCC450.003823538320.55555515
CGAACTA1650.020.18181629
ATTAGAG1303.274181E-1119.9230773
CCGCCAA656.8945315E-519.92307731
TAGGACC759.252686E-619.7333324
CATGCTA1800.019.5277774
GCTCCGA1457.2759576E-1219.13793229
Produced by FastQC (version 0.11.5)