##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633201.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577496
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89048755316054	33.0	33.0	33.0	33.0	33.0
2	32.003425131948966	33.0	33.0	33.0	33.0	33.0
3	31.72134871929849	33.0	33.0	33.0	27.0	33.0
4	32.0000138529098	33.0	33.0	33.0	33.0	33.0
5	32.099448307867064	33.0	33.0	33.0	33.0	33.0
6	35.28218030947401	37.0	37.0	37.0	33.0	37.0
7	35.430247135910896	37.0	37.0	37.0	33.0	37.0
8	35.55821858506379	37.0	37.0	37.0	33.0	37.0
9	35.65920456591907	37.0	37.0	37.0	33.0	37.0
10	35.67125486583457	37.0	37.0	37.0	33.0	37.0
11	35.69240133264992	37.0	37.0	37.0	33.0	37.0
12	35.69158920581268	37.0	37.0	37.0	33.0	37.0
13	35.6969693296577	37.0	37.0	37.0	33.0	37.0
14	35.679497347167775	37.0	37.0	37.0	33.0	37.0
15	35.68842035269508	37.0	37.0	37.0	33.0	37.0
16	35.690300885200934	37.0	37.0	37.0	33.0	37.0
17	35.67043581254242	37.0	37.0	37.0	33.0	37.0
18	35.65901408840927	37.0	37.0	37.0	33.0	37.0
19	35.6393083242135	37.0	37.0	37.0	33.0	37.0
20	35.655481596409324	37.0	37.0	37.0	33.0	37.0
21	35.64253258897031	37.0	37.0	37.0	33.0	37.0
22	35.56355541856567	37.0	37.0	37.0	33.0	37.0
23	35.60642497956696	37.0	37.0	37.0	33.0	37.0
24	35.58767679776137	37.0	37.0	37.0	33.0	37.0
25	35.61672981284719	37.0	37.0	37.0	33.0	37.0
26	35.50512038178619	37.0	37.0	37.0	33.0	37.0
27	35.52615948855057	37.0	37.0	37.0	33.0	37.0
28	35.53975438790918	37.0	37.0	37.0	33.0	37.0
29	35.54510507432086	37.0	37.0	37.0	33.0	37.0
30	35.54767998393063	37.0	37.0	37.0	33.0	37.0
31	35.540431448875836	37.0	37.0	37.0	33.0	37.0
32	35.53122619031127	37.0	37.0	37.0	33.0	37.0
33	35.525020086719216	37.0	37.0	37.0	33.0	37.0
34	35.49816448945101	37.0	37.0	37.0	33.0	37.0
35	35.42602026680704	37.0	37.0	37.0	33.0	37.0
36	35.451461135661546	37.0	37.0	37.0	33.0	37.0
37	35.442061936359735	37.0	37.0	37.0	33.0	37.0
38	35.429256652859934	37.0	37.0	37.0	33.0	37.0
39	35.32979795531051	37.0	37.0	37.0	33.0	37.0
40	35.12494112513333	37.0	37.0	37.0	33.0	37.0
41	35.275456453378034	37.0	37.0	37.0	33.0	37.0
42	35.322327773698866	37.0	37.0	37.0	33.0	37.0
43	34.99246228545306	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	18.0
16	18.0
17	18.0
18	33.0
19	20.0
20	34.0
21	101.0
22	214.0
23	527.0
24	1117.0
25	2048.0
26	3496.0
27	5448.0
28	7624.0
29	10212.0
30	13637.0
31	17584.0
32	22976.0
33	30093.0
34	43479.0
35	88217.0
36	330578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.28408854779946	21.712185019463337	14.222263011345534	23.781463421391663
2	15.178806433291314	22.52535082494078	39.43161511075402	22.864227631013893
3	17.042715447379724	28.237944505243327	31.117098646570714	23.60224140080624
4	11.69860916785571	18.403071190103482	41.54608863091692	28.352231011123884
5	12.967189383129925	37.79974233587765	36.082674165708504	13.150394115283914
6	27.569887929959684	41.57362128915179	17.99891254658041	12.857578234308118
7	24.89263994902129	33.355036225359136	24.53488855334063	17.21743527227894
8	24.022677213348665	35.89808414257415	21.5056035020156	18.57363514206159
9	25.679658387244242	15.457423081718316	21.350277750841563	37.51264078019588
10	14.016200978015434	29.087993683073133	34.96803441062795	21.927770928283486
11	32.247322925180434	24.769868535885962	25.43151814038539	17.551290398548215
12	21.863181736323714	26.677587377228583	31.621864047543184	19.837366838904515
13	27.31291645310097	22.984574784933574	27.1619197362406	22.540589025724852
14	21.023695402219236	21.888982780833114	29.541849640516993	27.545472176430657
15	22.774356878662363	29.97423358776511	25.614030227049188	21.637379306523336
16	21.353914139665036	29.75968664718024	26.54286782938756	22.34353138376716
17	21.199107872608643	28.591020543865238	27.86443542466095	22.34543615886517
18	21.792358734952277	28.023570726031004	29.425831520910968	20.758239018105755
19	23.461980689043735	27.295946638591435	29.07448709601452	20.167585576350312
20	23.077389280618394	27.47551498192195	28.885914361311592	20.56118137614806
21	21.90370149749955	27.829976311524234	29.058729411112804	21.20759277986341
22	21.32464986770471	27.8504093534847	29.390160278166427	21.43478050064416
23	21.524304930250597	27.585991937606497	29.396047764833007	21.493655367309902
24	21.530192416917174	27.871535040935346	28.72348899386316	21.87478354828432
25	21.829415268677185	28.029111889952485	29.035352625819055	21.10612021555128
26	21.89798717220552	27.807292171720672	28.964529624447614	21.330191031626196
27	21.687422943189215	28.011795752697854	28.774917921509413	21.525863382603518
28	21.464217933977032	27.85456522642581	29.19431476581656	21.4869020737806
29	22.185435050632385	27.734218072506128	28.859593832684556	21.22075304417693
30	22.04326956377187	27.711880255447657	28.90773269425243	21.337117486528044
31	21.622833751229447	27.879154141327383	28.970070788369096	21.52794131907407
32	21.059366644963777	27.617507307409923	29.32401263385374	21.999113413772562
33	20.864906423594277	27.842270768975023	29.704794492083064	21.58802831534764
34	21.735388643384542	27.55949824760691	29.116218986798177	21.58889412221037
35	21.571751146328285	28.01335420505077	28.804182193469742	21.610712455151205
36	22.023356005929045	27.761923892113536	28.81024284150886	21.404477260448555
37	22.11114882181002	27.32036239212047	28.81734245778326	21.751146328286257
38	21.788722346128804	27.761404407995897	28.733705514843393	21.716167731031906
39	21.403611453585825	27.686079210938257	29.07483341875961	21.835475916716305
40	21.38733428456647	27.857162647014004	29.241068336404062	21.51443473201546
41	21.35807001260615	27.294215024865977	29.186349342679428	22.16136561984845
42	21.325861997312536	27.84711928740632	29.21492096914957	21.612097746131575
43	21.67027996730713	27.345124468394587	29.120548021111837	21.864047543186444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	67.5
2	73.0
3	254.0
4	435.0
5	435.0
6	544.0
7	653.0
8	583.0
9	513.0
10	749.5
11	986.0
12	986.0
13	1535.0
14	2084.0
15	3632.5
16	5181.0
17	6086.0
18	6991.0
19	6991.0
20	7200.0
21	7409.0
22	8494.5
23	9580.0
24	11850.0
25	14120.0
26	14120.0
27	16523.5
28	18927.0
29	21546.5
30	24166.0
31	26668.0
32	29170.0
33	29170.0
34	32552.0
35	35934.0
36	37435.0
37	38936.0
38	39590.0
39	40244.0
40	40244.0
41	41057.0
42	41870.0
43	42023.5
44	42177.0
45	41375.5
46	40574.0
47	40574.0
48	39033.5
49	37493.0
50	36741.5
51	35990.0
52	34771.0
53	33552.0
54	33552.0
55	31207.0
56	28862.0
57	25456.5
58	22051.0
59	20515.5
60	18980.0
61	18980.0
62	16349.0
63	13718.0
64	11597.0
65	9476.0
66	7988.0
67	6500.0
68	6500.0
69	5407.5
70	4315.0
71	3624.5
72	2934.0
73	2229.0
74	1524.0
75	1524.0
76	1150.0
77	776.0
78	617.5
79	459.0
80	373.5
81	288.0
82	288.0
83	237.0
84	186.0
85	172.5
86	159.0
87	128.0
88	97.0
89	97.0
90	68.0
91	39.0
92	25.0
93	11.0
94	6.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	577496.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.93192703130501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.85029600521939	58.58071232925977
2	6.5613288154173315	8.65202105372989
3	1.5503464129391509	3.066519797134487
4	0.7645723954798074	2.01638925555699
5	0.44252857824387337	1.4588380965021108
6	0.3044173102909096	1.2042491933499837
7	0.209749752454077	0.9680443761545545
8	0.16042189738606588	0.8461539866145278
9	0.1313795098091689	0.7795893828732072
>10	0.8838502127930044	11.571361328280297
>50	0.09540145802492875	4.33537050330335
>100	0.04464468329244242	5.102960046676067
>500	2.657421624549621E-4	0.1291289804085774
>1k	7.972264873648863E-4	1.288661670156156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3501	0.606237965284608	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2552	0.4419078227381661	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1302	0.22545610705528696	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	737	0.12761993156662557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.7316137254630334E-4	0.0	0.0
2	0.0	0.0	1.7316137254630334E-4	0.0	0.0
3	0.0	0.0	1.7316137254630334E-4	0.0	0.0
4	0.0	0.0	1.7316137254630334E-4	1.7316137254630334E-4	0.0
5	0.0	0.0	1.7316137254630334E-4	1.7316137254630334E-4	0.0
6	0.0	0.0	3.463227450926067E-4	1.7316137254630334E-4	0.0
7	0.0	0.0	0.0027705819607408535	1.7316137254630334E-4	0.0
8	1.7316137254630334E-4	0.0	0.002943743333287157	1.7316137254630334E-4	0.0
9	1.7316137254630334E-4	0.0	0.0031169047058334605	0.0010389682352778202	0.0
10	3.463227450926067E-4	0.0	0.0036363888234723705	0.0010389682352778202	0.0
11	3.463227450926067E-4	0.0	0.005887486666574314	0.0015584523529167302	0.0
12	3.463227450926067E-4	0.0	0.006406970784213224	0.0015584523529167302	0.0
13	3.463227450926067E-4	0.0	0.007272777646944741	0.0015584523529167302	0.0
14	3.463227450926067E-4	0.0	0.007792261764583651	0.0019047750980093368	0.0
15	3.463227450926067E-4	0.0	0.008658068627315168	0.0019047750980093368	0.0
16	3.463227450926067E-4	0.0	0.010043359607685595	0.0019047750980093368	0.0
17	3.463227450926067E-4	0.0	0.010043359607685595	0.0019047750980093368	0.0
18	3.463227450926067E-4	0.0	0.010562843725324504	0.0019047750980093368	0.0
19	3.463227450926067E-4	0.0	0.011082327842963414	0.0019047750980093368	0.0
20	3.463227450926067E-4	0.0	0.01142865058805602	0.0020779364705556403	0.0
21	3.463227450926067E-4	0.0	0.01142865058805602	0.0022510978431019434	0.0
22	3.463227450926067E-4	0.0	0.011774973333148628	0.0025974205881945504	0.0
23	3.463227450926067E-4	0.0	0.012121296078241235	0.002943743333287157	0.0
24	3.463227450926067E-4	0.0	0.012121296078241235	0.0032900660783797635	0.0
25	3.463227450926067E-4	0.0	0.012121296078241235	0.0038095501960186736	0.0
26	3.463227450926067E-4	0.0	0.012121296078241235	0.004155872941111281	0.0
27	3.463227450926067E-4	0.0	0.012121296078241235	0.005541163921481707	0.0
28	3.463227450926067E-4	0.0	0.012121296078241235	0.016450330391898818	0.0
29	3.463227450926067E-4	0.0	0.012121296078241235	0.03671021097981631	0.0
30	3.463227450926067E-4	0.0	0.012121296078241235	0.06978403313616025	0.0
31	3.463227450926067E-4	0.0	0.012121296078241235	0.13142948176264424	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGC	25	1.2328652E-4	36.999996	28
TTTGGCG	30	3.5972794E-4	30.833334	22
CCGGGTC	25	0.005494107	29.599998	14
GCGCTCA	35	8.864029E-4	26.42857	31
GGTATCA	490	0.0	24.540815	1
AATACCG	55	1.9005338E-5	23.545454	5
GTATCAA	1430	0.0	23.286713	1
TTGGCGT	40	0.0019300296	23.125002	23
GGCACCG	140	0.0	22.464285	9
GCTCGGA	140	0.0	21.142857	11
AGGACCG	45	0.0038235383	20.555555	5
GCCGGCA	45	0.0038235383	20.555555	15
CGCTCAT	45	0.0038235383	20.555555	32
CGAGCCC	45	0.0038235383	20.555555	15
CGAACTA	165	0.0	20.181816	29
ATTAGAG	130	3.274181E-11	19.923077	3
CCGCCAA	65	6.8945315E-5	19.923077	31
TAGGACC	75	9.252686E-6	19.733332	4
CATGCTA	180	0.0	19.527777	4
GCTCCGA	145	7.2759576E-12	19.137932	29
>>END_MODULE