FastQCFastQC Report
Fri 10 Feb 2017
ERR1633200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381374
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123363.234620084221788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96232.5232448987083544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47691.250478532883731No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.6300901477290008No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG23560.6177662871616838No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19380.5081625910523528No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18910.4958387304850357No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC16480.43212174925401314No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16240.4258287140707023No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14860.38964376176666476No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.3681425582236859No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG12780.33510412351130386No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12730.3337930745147808No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12650.3316953961203438No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA11570.3033767377954449No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11310.2965592830135248No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC10000.2622097993046196No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC9630.2525080367303487No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9340.24490395255051473No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC9230.24201964475816393No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8950.23467777037763454No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8810.2310068331873699No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8340.21868297262005276No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8310.21789634322213888No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8120.21291435703535114No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8020.21029225904230492No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7670.20111491606664322No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7590.1990172376722063No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7450.1953463004819416No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC7350.1927242024888954No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.19010210449584922No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7240.1898398946965446No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.18748000650280303No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7040.1845956987104522No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC7040.1845956987104522No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7040.1845956987104522No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6950.18223581051671064No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6930.1817113909181014No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA6670.1748939361361813No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6640.1741073067382674No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6620.1735828871396582No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6540.17148520874522125No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6400.16781427155495654No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6390.16755206175565193No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA6380.16728985195634732No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6320.1657165931605196No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6310.16545438336121496No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6300.16519217356191035No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6270.1644055441639965No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.16361891476608265No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG6060.15889913837859948No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6060.15889913837859948No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6010.15758808938207639No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5950.15601483058624868No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5930.15549041098763944No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5740.15050842480085166No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5740.15050842480085166No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5700.14945958560363318No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5680.14893516600502393No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5630.14762411700850084No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5490.1439531798182362No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG5480.14369097001893155No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5470.14342876021962692No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5470.14342876021962692No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5460.1431665504203223No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5440.14264213082171306No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5410.1418555014237992No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5370.14080666222658073No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5230.13713572503631608No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5200.1363490956384022No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5170.13556246624048834No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5170.13556246624048834No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5150.1350380466418791No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA4990.13084268985300518No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4980.13058048005370057No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4920.12900722125787284No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4890.128220591859959No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4850.12717175266274053No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG4740.12428744487038969No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4740.12428744487038969No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4720.12376302527178046No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC4690.12297639587386659No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4590.12035429788082039No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.11851882928568806No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4520.11851882928568806No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA4520.11851882928568806No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG4500.11799440968707883No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4450.11668336069055572No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG4440.11642115089125112No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA4430.1161589410919465No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4420.11589673129264186No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA4400.11537231169403263No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4360.11432347249681414No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4330.11353684309890029No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4280.1122257941023772No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4220.11065253530654948No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4160.10907927651072176No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4150.10881706671141715No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG4100.10750601771489403No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA4070.10671938831698018No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4050.10619496871837095No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4030.1056705491197617No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4010.10514612952115246No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC3980.1043595001232386No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3940.10331066092602013No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.10226182172880165No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3900.10226182172880165No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3840.10068856293297393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCT308.297418E-637.035
TGTCAAC251.2320794E-436.9999962
TCCTGCT251.2320794E-436.99999637
TTCCCTG352.3841554E-531.71428536
TAGTTCC250.005491796429.5999984
GTGTGAA250.005491796429.5999981
GGGACCG250.005491796429.5999985
TAACAAC250.005491796429.5999981
GACCCAA453.99903E-628.77777735
ACAACGC405.9298844E-527.753
TTCCTGC405.9298844E-527.7536
GGTATCA15550.027.2443731
CTAGTCC754.6020432E-1027.1333339
ACTAGTC905.456968E-1226.7222218
TCTTCCC509.070305E-625.89999834
ATAGATC601.3334939E-624.6666666
TTATAGA601.3334939E-624.6666664
GTGACAT601.3334939E-624.66666616
GCCGGCA6550.024.00763315
GTATCAA43950.023.8668941
Produced by FastQC (version 0.11.5)