FastQCFastQC Report
Fri 10 Feb 2017
ERR1633199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449754
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31860.7083872516975948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.5053873895507321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12750.2834883069411278No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.13918720011384No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6050.134517980940692No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5990.1331839183197926No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5020.11161657261525189No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5000.11117188507495208No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT4830.10739204098240372No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4730.10516860328090467No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4600.10227813426895592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTT250.0054928329.65
CGCCCAT250.0054928329.635
ACGCGGC250.0054928329.610
TTAGACT405.9322134E-527.7500024
GCCGCTC556.247519E-726.9090927
ACCCGCA951.0913936E-1125.3157926
CCGCTCT601.3343833E-624.66666828
TTCGCCG601.3343833E-624.66666824
GGTATCA4950.024.6666641
CTAAGCC400.001929369423.1250024
AGACGTA400.001929369423.12500222
TACCGGG400.001929369423.12500232
GTATCAA12700.021.9960631
CCCGCAG1107.4578566E-1121.86363627
AGCTTCG855.1630195E-821.76470621
CGCTCTC705.0905346E-621.14285929
CCTAAGC450.003822241220.5555573
TATTAGA1450.020.4137942
ATAATAT1550.020.2903233
ATTGACG1202.382876E-1020.04166817
Produced by FastQC (version 0.11.5)