FastQCFastQC Report
Fri 10 Feb 2017
ERR1633198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664232
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33780.5085572510809476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24840.37396572281973767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13450.2024894916234087No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9690.14588276385359333No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC8820.1327849305664286No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC8740.13158053210324103No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA7790.11727830035288875No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7740.11652555131339652No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT7570.11396620457912296No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA7390.11125630803695094No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG6810.10252441917884114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.10252441917884114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG358.8654365E-426.42857213
TTCGCCG451.3223905E-424.66666824
GGTATCA6450.024.6666661
GCGCTGA400.001930333623.1251
GTATCAA16400.022.448171
TGGGGCG855.1724783E-821.76470637
GCTTCGC705.096572E-621.14285922
ACGGTAC450.003824132520.5555573
TCGCCAT1351.8189894E-1220.55555513
GCCGCTC656.896525E-519.92307727
GCCGGCA759.256119E-619.73333415
GTATAAC759.256119E-619.7333341
ATTACCC1355.638867E-1119.1851863
CCGGCAG801.6149967E-518.516
CCGCTCT609.231218E-418.528
CGCTCTC609.231218E-418.529
TAGGACC1205.162292E-918.54
ATTTCGG902.1494325E-618.529
ACGCACT500.007031833318.49999833
GTCGCCA1502.5102054E-1017.26666612
Produced by FastQC (version 0.11.5)