##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633198.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664232
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87439027327801	33.0	33.0	33.0	33.0	33.0
2	32.007405545050524	33.0	33.0	33.0	33.0	33.0
3	31.714139035758592	33.0	33.0	33.0	27.0	33.0
4	31.990152537065363	33.0	33.0	33.0	33.0	33.0
5	32.09151170073107	33.0	33.0	33.0	33.0	33.0
6	35.28804243095786	37.0	37.0	37.0	33.0	37.0
7	35.43719965313324	37.0	37.0	37.0	33.0	37.0
8	35.56097116670079	37.0	37.0	37.0	33.0	37.0
9	35.685816100398654	37.0	37.0	37.0	33.0	37.0
10	35.68312276433535	37.0	37.0	37.0	33.0	37.0
11	35.709905575160484	37.0	37.0	37.0	33.0	37.0
12	35.71423538763565	37.0	37.0	37.0	33.0	37.0
13	35.70553360873912	37.0	37.0	37.0	33.0	37.0
14	35.68375657902661	37.0	37.0	37.0	33.0	37.0
15	35.701369401052645	37.0	37.0	37.0	33.0	37.0
16	35.68339525948765	37.0	37.0	37.0	33.0	37.0
17	35.66946488576281	37.0	37.0	37.0	33.0	37.0
18	35.65849281576317	37.0	37.0	37.0	33.0	37.0
19	35.65818569415505	37.0	37.0	37.0	33.0	37.0
20	35.660659227498826	37.0	37.0	37.0	33.0	37.0
21	35.65164581049994	37.0	37.0	37.0	33.0	37.0
22	35.5696819785858	37.0	37.0	37.0	33.0	37.0
23	35.615712582350746	37.0	37.0	37.0	33.0	37.0
24	35.599343301737946	37.0	37.0	37.0	33.0	37.0
25	35.610925098459575	37.0	37.0	37.0	33.0	37.0
26	35.50575100266172	37.0	37.0	37.0	33.0	37.0
27	35.53078743571523	37.0	37.0	37.0	33.0	37.0
28	35.54808410314468	37.0	37.0	37.0	33.0	37.0
29	35.562267400546794	37.0	37.0	37.0	33.0	37.0
30	35.56295992966313	37.0	37.0	37.0	33.0	37.0
31	35.55068108733093	37.0	37.0	37.0	33.0	37.0
32	35.54038046947452	37.0	37.0	37.0	33.0	37.0
33	35.54257849666984	37.0	37.0	37.0	33.0	37.0
34	35.5190626166761	37.0	37.0	37.0	33.0	37.0
35	35.45315793277048	37.0	37.0	37.0	33.0	37.0
36	35.47459622541522	37.0	37.0	37.0	33.0	37.0
37	35.467601681340255	37.0	37.0	37.0	33.0	37.0
38	35.44823344855412	37.0	37.0	37.0	33.0	37.0
39	35.3485514097484	37.0	37.0	37.0	33.0	37.0
40	35.14731148153055	37.0	37.0	37.0	33.0	37.0
41	35.295959845355235	37.0	37.0	37.0	33.0	37.0
42	35.347063977646364	37.0	37.0	37.0	33.0	37.0
43	35.01623228028761	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	22.0
16	25.0
17	30.0
18	22.0
19	21.0
20	41.0
21	87.0
22	244.0
23	610.0
24	1220.0
25	2429.0
26	3900.0
27	5999.0
28	8642.0
29	11812.0
30	15662.0
31	20023.0
32	26019.0
33	34715.0
34	49692.0
35	102596.0
36	380417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.24693179491503	21.881360729383708	14.392712184899253	23.47899529080201
2	15.103006178564115	22.76237820520541	40.21697238314324	21.917643233087233
3	17.181346276602152	28.24630550771417	31.775042455045828	22.797305760637848
4	12.079363836731744	18.613074949716363	40.81390237146058	28.493658842091317
5	12.653410254248515	38.496338628671914	36.11810331330017	12.732147803779403
6	27.376428717676955	41.28135952498525	18.32778306374881	13.014428693588986
7	24.39418757301666	33.62831059027568	24.87037661539944	17.107125221308216
8	23.363071938720207	36.74273446627082	21.63536234327765	18.258831251731323
9	25.101470570523553	16.22294619952065	21.132074335473146	37.54350889448265
10	13.9523539967963	29.070415156150258	35.180479109708656	21.79675173734478
11	31.752610533668957	24.943543822038084	25.55296944441099	17.75087619988197
12	21.462681713618135	26.932306784376543	31.637590480434547	19.96742102157078
13	26.578665285623092	23.38580495971287	27.942947644798803	22.092582109865226
14	21.050024690168495	22.570126100519094	29.105041611966904	27.274807597345507
15	22.809048645653927	29.955798576401016	25.968938563634392	21.266214214310665
16	21.686248178347327	30.206614556359828	26.06950583531055	22.037631429982298
17	21.229781160799238	28.910531260162113	27.573046766792324	22.286640812246322
18	21.157818352623785	28.47679726360669	29.434595141456597	20.930789242312926
19	23.180003372315696	27.639288682267644	29.523419528116683	19.657288417299977
20	23.088318539305543	27.139613869852703	29.51995688253502	20.252110708306738
21	21.29466812800347	28.474388466680317	28.671759264835178	21.559184140481037
22	20.966620096592756	28.60777559647834	28.735592383384116	21.690011923544787
23	21.01946307916511	28.190752628599647	29.32499488130653	21.464789410928713
24	22.000746727047176	28.407845451589203	28.32429030820557	21.267117513158055
25	21.923213575979478	27.873393633549725	29.01350732876465	21.18988546170615
26	21.6788712377603	28.027707186645628	28.790693613074954	21.50272796251912
27	21.587638054173844	27.965981765407268	28.442773007021643	22.003607173397246
28	20.7591624613087	28.488991798046463	29.166164833973674	21.58568090667116
29	22.22687253851064	28.361626660564383	28.491400594972845	20.92010020595214
30	21.376266123884427	28.524521552710496	28.858290476821352	21.240921846583724
31	21.750984595743656	28.049687458598804	28.980085271411195	21.219242674246345
32	20.6909633983307	28.00557636488456	29.151260402991724	22.152199833793013
33	20.924165050765396	27.92322561996411	29.607426320924013	21.54518300834648
34	21.132525984896844	28.052849004564674	28.9410928711655	21.87353213937299
35	21.39433210083224	28.66362957520866	28.524371002902598	21.4176673210565
36	21.810150670247744	28.370207999614593	28.413415794481445	21.40622553565622
37	22.142865745703308	27.557540136578783	28.33543099399005	21.964163123727854
38	22.007069818978913	28.114423875995133	28.574202989316987	21.30430331570897
39	21.438744294162284	28.10599308675282	28.880120198966626	21.575142420118272
40	21.58522925724747	28.2342615230823	28.902702670151392	21.277806549518843
41	21.26365486757639	28.021233544906	29.116633947175085	21.59847764034253
42	21.460724566115456	28.034030278577365	28.803038697322624	21.70220645798456
43	21.369792482144792	27.785472545737033	28.733183586457745	22.111551385660434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	55.0
2	72.0
3	241.5
4	411.0
5	411.0
6	495.0
7	579.0
8	532.5
9	486.0
10	763.5
11	1041.0
12	1041.0
13	1589.0
14	2137.0
15	4105.0
16	6073.0
17	6968.0
18	7863.0
19	7863.0
20	7973.5
21	8084.0
22	9773.0
23	11462.0
24	13726.5
25	15991.0
26	15991.0
27	18879.0
28	21767.0
29	26269.0
30	30771.0
31	32557.0
32	34343.0
33	34343.0
34	37184.0
35	40025.0
36	41802.0
37	43579.0
38	45869.5
39	48160.0
40	48160.0
41	50193.5
42	52227.0
43	49597.5
44	46968.0
45	46769.5
46	46571.0
47	46571.0
48	44789.0
49	43007.0
50	43690.0
51	44373.0
52	44049.5
53	43726.0
54	43726.0
55	38436.5
56	33147.0
57	28876.5
58	24606.0
59	21319.5
60	18033.0
61	18033.0
62	15812.0
63	13591.0
64	11129.0
65	8667.0
66	7287.5
67	5908.0
68	5908.0
69	4882.5
70	3857.0
71	3322.0
72	2787.0
73	2242.0
74	1697.0
75	1697.0
76	1266.5
77	836.0
78	658.0
79	480.0
80	384.5
81	289.0
82	289.0
83	250.5
84	212.0
85	192.0
86	172.0
87	140.5
88	109.0
89	109.0
90	89.0
91	69.0
92	43.5
93	18.0
94	9.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	664232.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.89724794573563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.7826561552924	51.40271456703957
2	6.4709959839034745	7.493057178718378
3	1.5639479740997637	2.716448508920548
4	0.722421240628058	1.6730480675963448
5	0.43294119369775513	1.2533051818720842
6	0.27482887504669234	0.9547101312735565
7	0.21579660518639687	0.8745820689427388
8	0.14953269773096836	0.6926025341219691
9	0.13308671508245182	0.6934819087270968
>10	0.9890670037704428	11.906271003944626
>50	0.14874302062110714	6.0192428713107065
>100	0.11043439545588897	11.333630197491159
>500	0.004755548129678417	1.884502443289248
>1k	7.925913549464029E-4	1.102403336752079
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3378	0.5085572510809476	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2484	0.37396572281973767	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1345	0.2024894916234087	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	969	0.14588276385359333	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	882	0.1327849305664286	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	874	0.13158053210324103	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	779	0.11727830035288875	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	774	0.11652555131339652	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	757	0.11396620457912296	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	739	0.11125630803695094	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	681	0.10252441917884114	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	681	0.10252441917884114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	4.5164942369533537E-4	0.0	0.0
7	0.0	0.0	0.0018065976947813415	1.5054980789844512E-4	0.0
8	0.0	0.0	0.002408796926375122	3.0109961579689024E-4	0.0
9	0.0	0.0	0.0028604463500704574	4.5164942369533537E-4	0.0
10	1.5054980789844512E-4	0.0	0.003914295005359573	6.021992315937805E-4	0.0
11	1.5054980789844512E-4	0.0	0.005269243276445579	6.021992315937805E-4	0.0
12	1.5054980789844512E-4	0.0	0.006323091931734695	7.527490394922256E-4	0.0
13	1.5054980789844512E-4	0.0	0.006624191547531585	7.527490394922256E-4	0.0
14	1.5054980789844512E-4	0.0	0.006774741355430031	9.032988473906707E-4	0.0
15	1.5054980789844512E-4	0.0	0.007527490394922256	9.032988473906707E-4	0.0
16	1.5054980789844512E-4	0.0	0.008129689626516036	9.032988473906707E-4	0.0
17	1.5054980789844512E-4	0.0	0.008129689626516036	9.032988473906707E-4	0.0
18	1.5054980789844512E-4	0.0	0.008280239434414482	0.0010538486552891157	0.0
19	1.5054980789844512E-4	0.0	0.008280239434414482	0.001204398463187561	0.0
20	1.5054980789844512E-4	0.0	0.008430789242312926	0.001204398463187561	0.0
21	1.5054980789844512E-4	0.0	0.008731888858109818	0.001354948271086006	0.0
22	1.5054980789844512E-4	0.0	0.008882438666008262	0.0018065976947813415	0.0
23	1.5054980789844512E-4	0.0	0.008882438666008262	0.002258247118476677	0.0
24	1.5054980789844512E-4	0.0	0.008882438666008262	0.0030109961579689024	0.0
25	1.5054980789844512E-4	0.0	0.009183538281805152	0.003462645581664238	0.0
26	1.5054980789844512E-4	0.0	0.009183538281805152	0.004215394621156463	0.0
27	1.5054980789844512E-4	0.0	0.009183538281805152	0.006774741355430031	0.0
28	1.5054980789844512E-4	1.5054980789844512E-4	0.009183538281805152	0.016560478868828964	0.0
29	1.5054980789844512E-4	1.5054980789844512E-4	0.009183538281805152	0.03327150754555637	0.0
30	1.5054980789844512E-4	1.5054980789844512E-4	0.009183538281805152	0.06052102277517494	0.0
31	1.5054980789844512E-4	1.5054980789844512E-4	0.009183538281805152	0.1160739018897012	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAG	35	8.8654365E-4	26.428572	13
TTCGCCG	45	1.3223905E-4	24.666668	24
GGTATCA	645	0.0	24.666666	1
GCGCTGA	40	0.0019303336	23.125	1
GTATCAA	1640	0.0	22.44817	1
TGGGGCG	85	5.1724783E-8	21.764706	37
GCTTCGC	70	5.096572E-6	21.142859	22
ACGGTAC	45	0.0038241325	20.555557	3
TCGCCAT	135	1.8189894E-12	20.555555	13
GCCGCTC	65	6.896525E-5	19.923077	27
GCCGGCA	75	9.256119E-6	19.733334	15
GTATAAC	75	9.256119E-6	19.733334	1
ATTACCC	135	5.638867E-11	19.185186	3
CCGGCAG	80	1.6149967E-5	18.5	16
CCGCTCT	60	9.231218E-4	18.5	28
CGCTCTC	60	9.231218E-4	18.5	29
TAGGACC	120	5.162292E-9	18.5	4
ATTTCGG	90	2.1494325E-6	18.5	29
ACGCACT	50	0.0070318333	18.499998	33
GTCGCCA	150	2.5102054E-10	17.266666	12
>>END_MODULE