FastQCFastQC Report
Fri 10 Feb 2017
ERR1633197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591340
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38490.650894578415125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.45084046403084516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14780.24994081239219398No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7710.13038184462407415No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA7650.12936719991882845No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.1192207528663713No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6970.11786789325937701No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG6190.10467751209118274No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6130.10366286738593701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG251.232901E-436.99999624
CGGTAGG250.005494212729.59999829
CCGTCCG556.2531035E-726.9090928
GTATCAA14700.024.7925171
GCCGGCA601.335573E-624.66666815
GGTATCA4800.024.6666681
CGAGTCG701.9196341E-723.78571321
CGCCGTC701.9196341E-723.78571326
CGTCCGC701.9196341E-723.78571329
GCCGTCC701.9196341E-723.78571327
GCGTGCA400.00193008423.1250029
TCCGCCG753.7344216E-722.231
CGCCGCC855.1699317E-821.76470633
GGCGAGT909.458745E-820.55555519
GTCCGGC902.1486212E-618.515
GCGAGTC902.1486212E-618.520
AACGTCA1002.8690556E-718.49999828
TTCTGCG1002.8690556E-718.49999818
ACGCTTG852.719497E-517.41176425
TAATACG752.0651282E-417.2666662
Produced by FastQC (version 0.11.5)