FastQCFastQC Report
Fri 10 Feb 2017
ERR1633196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628222
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33440.5322959081343857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23730.3777327123214405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12730.20263537411934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.10314825014087377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGT250.005494470729.59999811
GGTATCA5700.023.368421
GTATCAA14550.021.2336771
TACTATC705.095848E-621.1428577
ACTATCG1151.3460522E-1020.91304411
CGCTCTC1151.3460522E-1020.91304429
CGAGTCG656.8957655E-519.92307721
TGCCGAG656.8957655E-519.92307711
CCGCTCT1254.110916E-1019.24000228
CGAACTA1205.160473E-918.529
GCCGCTC1205.160473E-918.527
ATAAGAC609.2304597E-418.53
TGCTCGC1409.276846E-1118.510
CGGATCA500.00703141918.49999823
CGATAGT500.00703141918.4999984
GCGAACT1351.1477823E-917.81481428
TTAACGG1054.788708E-717.61904735
AGGACCG650.001578856317.0769235
CGCCGCT1301.389526E-817.07692326
AGCTTCG1352.2135282E-816.44444321
Produced by FastQC (version 0.11.5)