##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633196.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	628222
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.913002091617294	33.0	33.0	33.0	33.0	33.0
2	32.02332296544852	33.0	33.0	33.0	33.0	33.0
3	31.738681548879217	33.0	33.0	33.0	27.0	33.0
4	32.01278369748274	33.0	33.0	33.0	33.0	33.0
5	32.11028903795155	33.0	33.0	33.0	33.0	33.0
6	35.301476229740445	37.0	37.0	37.0	33.0	37.0
7	35.45987246546603	37.0	37.0	37.0	33.0	37.0
8	35.58157944166234	37.0	37.0	37.0	33.0	37.0
9	35.703211603541426	37.0	37.0	37.0	33.0	37.0
10	35.70117569903633	37.0	37.0	37.0	33.0	37.0
11	35.71782268051739	37.0	37.0	37.0	33.0	37.0
12	35.72025653351841	37.0	37.0	37.0	33.0	37.0
13	35.72240545539634	37.0	37.0	37.0	33.0	37.0
14	35.7042462696308	37.0	37.0	37.0	33.0	37.0
15	35.709012100817866	37.0	37.0	37.0	33.0	37.0
16	35.69911591762148	37.0	37.0	37.0	33.0	37.0
17	35.69119355896483	37.0	37.0	37.0	33.0	37.0
18	35.67681647570445	37.0	37.0	37.0	33.0	37.0
19	35.673435505283166	37.0	37.0	37.0	33.0	37.0
20	35.67600147718482	37.0	37.0	37.0	33.0	37.0
21	35.676144738643345	37.0	37.0	37.0	33.0	37.0
22	35.5814473227617	37.0	37.0	37.0	33.0	37.0
23	35.62933485296599	37.0	37.0	37.0	33.0	37.0
24	35.605308314576696	37.0	37.0	37.0	33.0	37.0
25	35.63839693611494	37.0	37.0	37.0	33.0	37.0
26	35.523413697705585	37.0	37.0	37.0	33.0	37.0
27	35.539223395551254	37.0	37.0	37.0	33.0	37.0
28	35.56576337664074	37.0	37.0	37.0	33.0	37.0
29	35.56570607205733	37.0	37.0	37.0	33.0	37.0
30	35.57920130145076	37.0	37.0	37.0	33.0	37.0
31	35.56774993553234	37.0	37.0	37.0	33.0	37.0
32	35.543884168335396	37.0	37.0	37.0	33.0	37.0
33	35.54962099385249	37.0	37.0	37.0	33.0	37.0
34	35.51911267036175	37.0	37.0	37.0	33.0	37.0
35	35.45912432229371	37.0	37.0	37.0	33.0	37.0
36	35.487270423512705	37.0	37.0	37.0	33.0	37.0
37	35.48060080672119	37.0	37.0	37.0	33.0	37.0
38	35.472438087173	37.0	37.0	37.0	33.0	37.0
39	35.364718204711075	37.0	37.0	37.0	33.0	37.0
40	35.15710051542289	37.0	37.0	37.0	33.0	37.0
41	35.31430768104269	37.0	37.0	37.0	33.0	37.0
42	35.349925663220965	37.0	37.0	37.0	33.0	37.0
43	35.01759409890134	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	14.0
16	21.0
17	21.0
18	17.0
19	19.0
20	36.0
21	75.0
22	235.0
23	554.0
24	1208.0
25	2207.0
26	3697.0
27	5636.0
28	7973.0
29	10927.0
30	14581.0
31	18984.0
32	24063.0
33	32366.0
34	46882.0
35	97232.0
36	361469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.62974553581377	21.302501345065917	13.819637007299967	24.248116111820345
2	15.455842043099413	22.099194233885473	39.09398270038299	23.350981022632126
3	17.36870087325818	27.88250013530249	30.409791443152262	24.33900754828707
4	12.155416397388183	18.295284151144024	40.27318368347495	29.276115767992845
5	13.364861466169604	37.55487709758652	35.769680144916926	13.310581291326951
6	28.560604372339714	40.62735784483829	17.84003107181856	12.972006711003434
7	25.652874302396288	32.847623929120594	24.122205207713197	17.37729656076992
8	24.11026675283578	36.10873226343554	21.14761979045624	18.63338119327244
9	25.64953153503061	15.441993435441612	20.826554943952935	38.08192008557484
10	14.442187634307619	28.533384695219205	34.46886610147368	22.555561568999494
11	33.089258255839496	24.09705486277144	24.785505760702428	18.028181120686636
12	22.287344282753548	26.18564774872577	31.028362585200775	20.498645383319907
13	27.46751944376351	22.998557834650807	26.71396417190102	22.819958549684667
14	21.633912852462984	21.447195418180197	28.592758610809554	28.326133118547265
15	23.327422471674026	29.53732916071071	24.90043328632235	22.23481508129292
16	22.034885756945794	29.29649073098363	25.95181321252678	22.71681029954379
17	21.804393988112484	28.120950874054074	27.124806199082492	22.949848938750954
18	22.294984893875096	27.84031759473562	28.502981430131385	21.3617160812579
19	23.736672704871843	27.056040698988575	28.314672201864944	20.892614394274638
20	23.332357033023357	26.954643422229722	28.39187421007224	21.321125334674683
21	22.34703655714063	27.61858069281241	28.262461359201048	21.77192139084591
22	22.027722684019345	27.27602662752976	28.359401612805662	22.336849075645233
23	22.166367939995734	27.27411647474937	28.64321848009143	21.916297105163462
24	22.29530325267183	27.683844246142286	27.624151971755207	22.39670052943068
25	22.3651830085543	27.449850530544932	28.474010779628856	21.71095568127191
26	22.441429940371396	27.575124717058618	28.260073668225566	21.72337167434442
27	22.432834252859656	27.44427925160214	27.892050899204424	22.23083559633378
28	21.86663313287341	27.34463294822531	28.46891703888116	22.31981688002012
29	23.010496289528223	27.348453253786083	27.837929903760134	21.80312055292556
30	22.82155034366832	27.091856063620824	28.23699265546256	21.849600937248297
31	22.36629726434286	27.15632371995887	28.326133118547265	22.151245897151007
32	21.708408810898057	27.1755844271611	28.43771787680151	22.67828888513933
33	21.643781975161648	27.338743310485786	28.781704556669457	22.235770157683113
34	22.130870934160217	27.178449656331676	28.530519466048627	22.16015994345948
35	22.172416757133625	27.6343394532506	28.125885435403408	22.067358354212367
36	22.675901194163846	27.417855471473462	27.910038171219725	21.996205163142964
37	22.30342140198847	27.115414614578924	28.121428412249173	22.459735571183433
38	22.41771221001493	27.402574249230366	27.833950418800995	22.345763121953706
39	21.983470811273722	27.376150469101685	28.22521337998351	22.41516533964108
40	22.111291868161256	27.800522745144235	28.22378076539822	21.864404621296295
41	22.168118913377754	26.950504757872217	28.21200148991917	22.66937483883086
42	21.777174310991974	27.74703846729341	28.193855038505493	22.281932183209122
43	22.297531764248944	26.988230275284852	28.201018111431946	22.513219849034257
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	37.0
2	58.0
3	226.5
4	395.0
5	395.0
6	468.5
7	542.0
8	496.5
9	451.0
10	643.5
11	836.0
12	836.0
13	1385.5
14	1935.0
15	3314.5
16	4694.0
17	5491.0
18	6288.0
19	6288.0
20	6169.5
21	6051.0
22	6743.5
23	7436.0
24	9292.0
25	11148.0
26	11148.0
27	13684.0
28	16220.0
29	18969.5
30	21719.0
31	24636.5
32	27554.0
33	27554.0
34	31676.0
35	35798.0
36	38185.5
37	40573.0
38	43180.0
39	45787.0
40	45787.0
41	47172.5
42	48558.0
43	49234.0
44	49910.0
45	48671.5
46	47433.0
47	47433.0
48	45902.0
49	44371.0
50	43867.5
51	43364.0
52	41188.5
53	39013.0
54	39013.0
55	36792.0
56	34571.0
57	30285.5
58	26000.0
59	23898.0
60	21796.0
61	21796.0
62	18898.0
63	16000.0
64	13286.0
65	10572.0
66	8936.5
67	7301.0
68	7301.0
69	5990.5
70	4680.0
71	3849.0
72	3018.0
73	2404.0
74	1790.0
75	1790.0
76	1376.0
77	962.0
78	741.0
79	520.0
80	419.5
81	319.0
82	319.0
83	280.5
84	242.0
85	205.5
86	169.0
87	125.5
88	82.0
89	82.0
90	61.0
91	40.0
92	25.0
93	10.0
94	5.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	628222.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.27424887158872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.07076404725858	56.834787218601356
2	7.613063930186831	9.93874059308661
3	1.9889082985024442	3.894734857776499
4	0.9172876021843086	2.3950103692720575
5	0.5454571635110538	1.7802153319905696
6	0.32022309299287544	1.2541393119868112
7	0.24090420759390105	1.100738884048802
8	0.18138250770757489	0.9471685559245683
9	0.1445005922458655	0.8488950858313731
>10	0.848181782896734	10.452958398616072
>50	0.08113661139415553	3.6268859804813993
>100	0.04646908625697122	5.454847315235231
>500	9.834727250103278E-4	0.3490627235459188
>1k	7.37604543757746E-4	1.1218153736027459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3344	0.5322959081343857	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2373	0.3777327123214405	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1273	0.20263537411934	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.10314825014087377	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.5917939836554594E-4	0.0	0.0
2	0.0	0.0	1.5917939836554594E-4	0.0	0.0
3	0.0	0.0	1.5917939836554594E-4	0.0	0.0
4	0.0	0.0	1.5917939836554594E-4	1.5917939836554594E-4	0.0
5	0.0	0.0	4.775381950966378E-4	1.5917939836554594E-4	0.0
6	0.0	0.0	7.958969918277297E-4	1.5917939836554594E-4	0.0
7	0.0	0.0	0.002387690975483189	1.5917939836554594E-4	0.0
8	0.0	0.0	0.002546870373848735	1.5917939836554594E-4	0.0
9	0.0	0.0	0.0036611261624075564	6.367175934621838E-4	0.0
10	1.5917939836554594E-4	0.0	0.003979484959138649	6.367175934621838E-4	0.0
11	1.5917939836554594E-4	0.0	0.005571278942794108	6.367175934621838E-4	0.0
12	1.5917939836554594E-4	0.0	0.0066855347313529295	6.367175934621838E-4	0.0
13	1.5917939836554594E-4	0.0	0.007163072926449568	6.367175934621838E-4	0.0
14	1.5917939836554594E-4	0.0	0.007481431723180659	6.367175934621838E-4	0.0
15	1.5917939836554594E-4	0.0	0.00859568751173948	6.367175934621838E-4	0.0
16	1.5917939836554594E-4	0.0	0.009232405105201664	7.958969918277297E-4	0.0
17	1.5917939836554594E-4	0.0	0.00939158450356721	9.550763901932756E-4	0.0
18	1.5917939836554594E-4	0.0	0.010028302097029394	9.550763901932756E-4	0.0
19	1.5917939836554594E-4	0.0	0.01018748149539494	9.550763901932756E-4	0.0
20	1.5917939836554594E-4	0.0	0.010346660893760486	9.550763901932756E-4	0.0
21	1.5917939836554594E-4	0.0	0.010346660893760486	0.0011142557885588215	0.0
22	1.5917939836554594E-4	0.0	0.010505840292126032	0.0014326145852899133	0.0
23	1.5917939836554594E-4	0.0	0.010505840292126032	0.0014326145852899133	0.0
24	1.5917939836554594E-4	0.0	0.010665019690491578	0.0017509733820210054	0.0
25	1.5917939836554594E-4	0.0	0.010665019690491578	0.0019101527803865512	0.0
26	1.5917939836554594E-4	0.0	0.010824199088857124	0.002706049772214281	0.0
27	1.5917939836554594E-4	0.0	0.010824199088857124	0.004934561349331924	0.0
28	1.5917939836554594E-4	0.0	0.010824199088857124	0.01448532525126468	0.0
29	1.5917939836554594E-4	0.0	0.010824199088857124	0.032790956063302464	0.0
30	1.5917939836554594E-4	0.0	0.010824199088857124	0.05634950702140326	0.0
31	1.5917939836554594E-4	0.0	0.010824199088857124	0.11492752561992417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGCGT	25	0.0054944707	29.599998	11
GGTATCA	570	0.0	23.36842	1
GTATCAA	1455	0.0	21.233677	1
TACTATC	70	5.095848E-6	21.142857	7
ACTATCG	115	1.3460522E-10	20.913044	11
CGCTCTC	115	1.3460522E-10	20.913044	29
CGAGTCG	65	6.8957655E-5	19.923077	21
TGCCGAG	65	6.8957655E-5	19.923077	11
CCGCTCT	125	4.110916E-10	19.240002	28
CGAACTA	120	5.160473E-9	18.5	29
GCCGCTC	120	5.160473E-9	18.5	27
ATAAGAC	60	9.2304597E-4	18.5	3
TGCTCGC	140	9.276846E-11	18.5	10
CGGATCA	50	0.007031419	18.499998	23
CGATAGT	50	0.007031419	18.499998	4
GCGAACT	135	1.1477823E-9	17.814814	28
TTAACGG	105	4.788708E-7	17.619047	35
AGGACCG	65	0.0015788563	17.076923	5
CGCCGCT	130	1.389526E-8	17.076923	26
AGCTTCG	135	2.2135282E-8	16.444443	21
>>END_MODULE