FastQCFastQC Report
Fri 10 Feb 2017
ERR1633195.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633195.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647052
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60310.9320734655019999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43200.6676434042395356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23040.3560764822610857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.19704753250125184No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7260.11220118321247752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGT801.8189894E-1230.062526
GTCGACA250.005494589529.622
TACGCGG250.005494589529.66
TCGACAT250.005494589529.623
ACGCGGG250.005494589529.67
TGCGGGT1000.027.75000221
GCGGGTA905.456968E-1226.72222122
CGTCAAT1000.025.90000230
GGTATCA9350.025.1283421
TTCTGCG1002.0008883E-1124.05000118
ACGGCGT400.001930280423.12535
CTGCGGG1054.0017767E-1122.9047620
CGAGTCG753.7355494E-722.19999921
GTATCAA24700.022.1700421
TCTGCGG1107.4578566E-1121.86363619
GGTAACG1151.3460522E-1020.91304425
TAACGTC1151.3460522E-1020.91304427
CGCCGTC806.9504495E-720.812526
CGGCGTG450.00382402820.55555536
GCGAGTC909.462201E-820.55555520
Produced by FastQC (version 0.11.5)