FastQCFastQC Report
Fri 10 Feb 2017
ERR1633194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483247
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141092.919624953698626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109372.2632318462401217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55191.1420660655937853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.5941061196448193No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG25510.5278873950588415No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA24040.497468168452158No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT20690.4281454411512125No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19670.4070382226894321No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18030.3731011263391185No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC17820.36875552253816374No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.3143320082690632No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14670.30357146552384184No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14580.30170906389486124No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG14110.29198318872129575No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13870.2870167843773474No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC11680.24169834473881888No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11070.22907540036461682No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA10950.2265921981926427No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10760.22266046142035023No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC10570.21872872464805782No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10550.21831485761939548No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC9540.19741457267194623No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9510.19679377212895266No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9490.19637990510029033No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA9390.1943105699569785No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9340.19327590238532263No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9240.19120656724201082No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9240.19120656724201082No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8840.1829292266687636No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.1829292266687636No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8690.17982522395379588No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8680.1796182904394647No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8620.1783766893534776No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.17796282232481525No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8550.17692815475315934No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT8380.1734102850095293No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8030.16616761200793798No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC8010.1657537449792756No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7970.1649260109219509No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7920.16389134335029498No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.1636844098359638No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7890.16327054280730144No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7790.16120120766398963No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7740.16016654009233375No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7630.15789027143469075No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7470.15457933520539185No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC7400.15313080060507359No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC7230.14961293086144353No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7220.14940599734711235No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA7140.1477505292324629No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT7130.14754359571813172No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT7080.14650892814647581No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG6940.1436118589458393No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6860.14195639083118985No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6800.14071478974520277No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6670.1380246540588974No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA6640.13740385351590387No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6620.1369899864872415No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6600.13657611945857914No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6500.13450678431526736No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA6500.13450678431526736No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6460.13367905025794263No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6250.12933344645698783No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6240.12912651294265665No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6180.12788491185666956No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG6150.12726411131367604No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6140.12705717779934483No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6090.12602251022768896No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6040.12498784265603305No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5970.12353930805571478No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5830.12064223885507824No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5740.11877983722609763No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG5710.1181590366831041No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5690.11774516965444173No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG5550.1148481004538052No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5540.11464116693947402No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5510.11402036639648049No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5430.11236489828183104No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC5400.11174409773883748No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5360.11091636368151275No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5330.11029556313851922No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5310.10988169610985686No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5280.10926089556686333No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5260.10884702853820097No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5170.10698462690922034No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA5100.10553609230890208No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5100.10553609230890208No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.10532915879457089No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.10512222528023972No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5080.10512222528023972No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5040.104294491222915No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5010.10367369067992144No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4990.10325982365125908No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4950.10243208959393435No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA4900.10139742202227846No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4880.1009835549936161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT308.3020095E-637.037
TAGACCG251.2325672E-436.9999965
CGAGCCC401.5975274E-632.37515
ATCTCAG352.3854642E-531.71428729
GAGACAT352.3854642E-531.71428724
TCCGGAC303.5964177E-430.83333430
CCAATCC303.5964177E-430.83333426
CTGCCCC303.5964177E-430.83333420
GACCGTT1050.029.952387
TGTCACT250.00549323129.59999828
AGCAACG250.00549323129.5999982
ATGTGTC250.00549323129.59999825
TAAACAT250.00549323129.59999820
ATACTGC250.00549323129.5999986
TAGCAAC250.00549323129.5999981
CTAGTCG250.00549323129.5999989
CGAAGAT250.00549323129.59999815
AGCCCAC405.9331127E-527.75000217
CATCTCA405.9331127E-527.75000228
ACCGTAC405.9331127E-527.7500028
Produced by FastQC (version 0.11.5)