FastQCFastQC Report
Fri 10 Feb 2017
ERR1633193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644996
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24580.38108763465199785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.27116447233781293No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14020.21736568908954473No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13950.2162804110413088No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG13860.2148850535507197No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12090.18744302290246762No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11260.17457472604481267No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11150.17286928911187047No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10130.15705523755186077No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9820.15224900619538725No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT9180.14232646404008706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9070.14062102710714486No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT8810.13658999435655414No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT8790.13627991491420102No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA8630.13379927937537597No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8540.1324039218847869No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG8410.13038840550949155No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8370.12976824662478528No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8300.12868296857654932No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7980.12372169749889923No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7690.11922554558477882No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7630.11829530725771943No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7580.11752010865183661No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7360.11410923478595215No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7270.11271387729536307No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7080.10976812259300833No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7070.10961308287183176No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6530.10124093792829723No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6520.10108589820712066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4500.025.0777781
GTATCAA13400.023.0559711
GTCCGGC603.723024E-521.58333215
ACGCTAC450.003824014420.55555730
GACGCTA450.003824014420.55555729
GTCGGTA450.003824014420.55555720
TTCTGCG656.896127E-519.92307718
TTGGCGT759.255435E-619.73333423
TACAGTC759.255435E-619.7333347
GGCCGTT2850.019.47368614
TGGCGTA701.2181446E-418.524
TAGCGGA801.6148777E-518.59
TTAGTAC801.6148777E-518.53
CGATAAG500.007031616318.512
CCCGCCA500.007031616318.524
CAAGCAT2050.018.04878237
CGGCCGT3000.017.88333313
TAGTACT1258.558345E-917.764
GCCGTTA3050.017.59016215
CCGTTAA2950.017.55932416
Produced by FastQC (version 0.11.5)