##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633193.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644996
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.898276888538845	33.0	33.0	33.0	33.0	33.0
2	32.00927447612078	33.0	33.0	33.0	33.0	33.0
3	31.716060254637238	33.0	33.0	33.0	27.0	33.0
4	32.00030542825072	33.0	33.0	33.0	33.0	33.0
5	32.10741151883112	33.0	33.0	33.0	33.0	33.0
6	35.290079318321354	37.0	37.0	37.0	33.0	37.0
7	35.44220274234259	37.0	37.0	37.0	33.0	37.0
8	35.55865462731552	37.0	37.0	37.0	33.0	37.0
9	35.677139083033076	37.0	37.0	37.0	33.0	37.0
10	35.675689461640076	37.0	37.0	37.0	33.0	37.0
11	35.69445236869686	37.0	37.0	37.0	33.0	37.0
12	35.684762386123325	37.0	37.0	37.0	33.0	37.0
13	35.69241204596618	37.0	37.0	37.0	33.0	37.0
14	35.66699948526813	37.0	37.0	37.0	33.0	37.0
15	35.6811484102227	37.0	37.0	37.0	33.0	37.0
16	35.66895143535774	37.0	37.0	37.0	33.0	37.0
17	35.65745834082692	37.0	37.0	37.0	33.0	37.0
18	35.645520282296324	37.0	37.0	37.0	33.0	37.0
19	35.63887838064112	37.0	37.0	37.0	33.0	37.0
20	35.631321744630974	37.0	37.0	37.0	33.0	37.0
21	35.63791868476704	37.0	37.0	37.0	33.0	37.0
22	35.54474291313434	37.0	37.0	37.0	33.0	37.0
23	35.601766522583084	37.0	37.0	37.0	33.0	37.0
24	35.57760513243493	37.0	37.0	37.0	33.0	37.0
25	35.6139185359289	37.0	37.0	37.0	33.0	37.0
26	35.50219691284907	37.0	37.0	37.0	33.0	37.0
27	35.52276758305478	37.0	37.0	37.0	33.0	37.0
28	35.542311890306294	37.0	37.0	37.0	33.0	37.0
29	35.547597194401206	37.0	37.0	37.0	33.0	37.0
30	35.54670571600444	37.0	37.0	37.0	33.0	37.0
31	35.52771800135195	37.0	37.0	37.0	33.0	37.0
32	35.52342805226699	37.0	37.0	37.0	33.0	37.0
33	35.54084056335233	37.0	37.0	37.0	33.0	37.0
34	35.51365744903844	37.0	37.0	37.0	33.0	37.0
35	35.44892526465281	37.0	37.0	37.0	33.0	37.0
36	35.47770683849202	37.0	37.0	37.0	33.0	37.0
37	35.482675861555734	37.0	37.0	37.0	33.0	37.0
38	35.46455947013625	37.0	37.0	37.0	33.0	37.0
39	35.38285818826783	37.0	37.0	37.0	33.0	37.0
40	35.17454371810058	37.0	37.0	37.0	33.0	37.0
41	35.33875868997637	37.0	37.0	37.0	33.0	37.0
42	35.3880458173384	37.0	37.0	37.0	33.0	37.0
43	35.061249992248015	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	17.0
16	26.0
17	27.0
18	12.0
19	22.0
20	32.0
21	72.0
22	252.0
23	603.0
24	1223.0
25	2387.0
26	3836.0
27	5913.0
28	8404.0
29	11412.0
30	15017.0
31	19410.0
32	25374.0
33	33214.0
34	48777.0
35	100401.0
36	368564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.10350451785747	21.69517330340033	13.514502415518855	24.68681976322334
2	16.0864563501169	21.402458309818975	38.913109538663804	23.59797580140032
3	16.8988644890821	28.238159616493746	29.836774181545312	25.026201712878837
4	11.945190357769661	18.281353682813535	40.93172670838269	28.841729251034113
5	12.809846882771367	38.26969469578106	36.33123306191046	12.589225359537112
6	28.320795787880854	41.24475190543817	17.650342017624915	12.784110289056056
7	25.95752531798647	32.394929580958646	24.537206432287952	17.11033866876694
8	23.704488089848617	36.37061315108931	20.83020669895627	19.0946920601058
9	25.289149079994296	15.619476709933085	20.89253266686925	38.19884154320337
10	14.925208838504425	27.934901921872385	34.44253917853754	22.69735006108565
11	32.8662503333354	24.369143374532555	24.618757325626824	18.14584896650522
12	22.61238829388089	26.69148335803633	29.72018431122053	20.97594403686224
13	26.39272181532909	22.959677269316398	26.621870523228054	24.025730392126462
14	21.369124769766014	21.325403568394222	28.214903658317258	29.090568003522506
15	23.64712339301329	29.175684810448438	25.144807099578912	22.03238469695936
16	22.608202221409123	28.911342085842396	25.404188553107304	23.07626713964118
17	21.836879608555712	28.37149377670559	26.40714050939851	23.384486105340187
18	21.837654807161595	28.518626472102156	28.247772079206694	21.39594664152956
19	24.263251244968963	26.703576456288104	27.773660611848754	21.259511686894182
20	23.005724066505838	27.189936061618987	28.21846957190432	21.585870299970853
21	22.269750510080684	27.806218953295836	28.240795291753745	21.683235244869735
22	22.058586409838203	27.604822355487475	27.77629628710876	22.560294947565566
23	22.04773362935584	27.71660599445578	28.4733548735186	21.762305502669786
24	22.490682112757288	28.4164552958468	26.25070543073135	22.842157160664563
25	22.22649442787242	27.693970195164002	28.242655768407865	21.836879608555712
26	22.34122382154308	28.04110413087833	28.22668667712668	21.390985370451908
27	22.319828340020713	27.9440492654218	26.706522210990457	23.02960018356703
28	21.663545200280314	27.477069625237988	28.917543674689455	21.941841499792247
29	24.070381831825312	27.134431841437777	27.153191647700144	21.64199467903677
30	22.979677393348176	26.829933829047004	27.946529900960627	22.243858876644197
31	22.212850932408884	27.438774814107376	28.15133737263487	22.197036880848874
32	21.278581572598902	27.075981866554212	28.43645541987857	23.208981140968316
33	21.159045947571766	27.479705300497987	28.794907255238794	22.566341496691454
34	22.724637052012724	26.999392244292984	28.632270587724573	21.64370011596971
35	22.557659272305564	27.919087870312374	27.503271338116825	22.019981519265237
36	23.716426148379217	27.528697852389783	26.792879335685804	21.9619966635452
37	22.240603042499487	26.709312925971634	27.670094078102807	23.379989953426065
38	22.606961903639714	27.60544251437218	27.43272826498149	22.354867317006615
39	21.60307970902145	27.70203226066518	28.040018852830094	22.65486917748327
40	22.05284994015467	28.296454551656137	28.142034989364273	21.508660518824925
41	21.73145259815565	26.895670670825865	28.150562174028988	23.222314556989502
42	21.489125513956676	28.2536635886114	27.78575371010053	22.471457187331396
43	22.909134320212836	26.40745058884086	27.88342873444176	22.799986356504537
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	43.0
2	51.0
3	182.5
4	314.0
5	314.0
6	349.0
7	384.0
8	348.5
9	313.0
10	440.0
11	567.0
12	567.0
13	923.5
14	1280.0
15	2299.5
16	3319.0
17	3839.5
18	4360.0
19	4360.0
20	4163.5
21	3967.0
22	4523.0
23	5079.0
24	6813.0
25	8547.0
26	8547.0
27	11241.5
28	13936.0
29	16398.0
30	18860.0
31	22704.5
32	26549.0
33	26549.0
34	33372.5
35	40196.0
36	43651.0
37	47106.0
38	50292.0
39	53478.0
40	53478.0
41	55875.0
42	58272.0
43	58410.0
44	58548.0
45	55591.5
46	52635.0
47	52635.0
48	48594.0
49	44553.0
50	44141.5
51	43730.0
52	40369.0
53	37008.0
54	37008.0
55	36626.0
56	36244.0
57	29854.5
58	23465.0
59	21549.5
60	19634.0
61	19634.0
62	17596.5
63	15559.0
64	12271.0
65	8983.0
66	7304.0
67	5625.0
68	5625.0
69	4673.0
70	3721.0
71	3176.0
72	2631.0
73	2621.0
74	2611.0
75	2611.0
76	2248.0
77	1885.0
78	1382.0
79	879.0
80	583.0
81	287.0
82	287.0
83	230.5
84	174.0
85	144.0
86	114.0
87	90.0
88	66.0
89	66.0
90	46.5
91	27.0
92	15.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	644996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.57841694189568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50308432060336	42.02182896886229
2	7.254285675803319	7.048034283495902
3	1.9241011891541882	2.8040936941538823
4	0.9063909726316861	1.7612415432348962
5	0.5441945496826578	1.3218054865995654
6	0.37349029854433907	1.0886140467863996
7	0.2813482116437982	0.9567215511762395
8	0.21054833012612703	0.8182483653829515
9	0.18295327697788358	0.7998822512925977
>10	1.4467804246927667	14.138357462548878
>50	0.19972647400415874	6.841372335810422
>100	0.15783262702061132	14.213773281880068
>500	0.01234082268885438	4.158304876618216
>1k	0.0029228264263076163	2.027721852157748
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2458	0.38108763465199785	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1749	0.27116447233781293	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1402	0.21736568908954473	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1395	0.2162804110413088	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1386	0.2148850535507197	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1209	0.18744302290246762	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1126	0.17457472604481267	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1115	0.17286928911187047	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1013	0.15705523755186077	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	982	0.15224900619538725	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	918	0.14232646404008706	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	907	0.14062102710714486	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	881	0.13658999435655414	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	879	0.13627991491420102	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	863	0.13379927937537597	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	854	0.1324039218847869	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	841	0.13038840550949155	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	837	0.12976824662478528	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	830	0.12868296857654932	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	798	0.12372169749889923	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	769	0.11922554558477882	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	763	0.11829530725771943	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	758	0.11752010865183661	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	736	0.11410923478595215	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	727	0.11271387729536307	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	708	0.10976812259300833	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	707	0.10961308287183176	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	653	0.10124093792829723	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	652	0.10108589820712066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.201588847062617E-4	0.0	0.0
2	3.1007944235313085E-4	4.651191635296963E-4	6.201588847062617E-4	0.0	0.0
3	3.1007944235313085E-4	4.651191635296963E-4	6.201588847062617E-4	0.0	0.0
4	3.1007944235313085E-4	4.651191635296963E-4	7.751986058828271E-4	0.0	0.0
5	3.1007944235313085E-4	4.651191635296963E-4	9.302383270593926E-4	0.0	0.0
6	3.1007944235313085E-4	4.651191635296963E-4	0.0012403177694125234	0.0	0.0
7	3.1007944235313085E-4	4.651191635296963E-4	0.0023255958176484817	1.5503972117656542E-4	0.0
8	4.651191635296963E-4	4.651191635296963E-4	0.002790714981178178	1.5503972117656542E-4	0.0
9	4.651191635296963E-4	4.651191635296963E-4	0.0034108738658844397	6.201588847062617E-4	0.0
10	4.651191635296963E-4	4.651191635296963E-4	0.0037209533082375704	6.201588847062617E-4	0.0
11	4.651191635296963E-4	4.651191635296963E-4	0.006046549125886052	6.201588847062617E-4	0.0
12	4.651191635296963E-4	4.651191635296963E-4	0.006356628568239183	6.201588847062617E-4	0.0
13	4.651191635296963E-4	4.651191635296963E-4	0.006666708010592314	6.201588847062617E-4	0.0
14	4.651191635296963E-4	4.651191635296963E-4	0.006821747731768879	6.201588847062617E-4	0.0
15	4.651191635296963E-4	4.651191635296963E-4	0.007286866895298575	6.201588847062617E-4	0.0
16	4.651191635296963E-4	4.651191635296963E-4	0.007441906616475141	6.201588847062617E-4	0.0
17	4.651191635296963E-4	4.651191635296963E-4	0.007751986058828272	9.302383270593926E-4	0.0
18	4.651191635296963E-4	4.651191635296963E-4	0.007907025780004837	0.0012403177694125234	0.0
19	4.651191635296963E-4	4.651191635296963E-4	0.008062065501181402	0.0012403177694125234	0.0
20	4.651191635296963E-4	4.651191635296963E-4	0.008527184664711099	0.0015503972117656543	0.0
21	4.651191635296963E-4	4.651191635296963E-4	0.008527184664711099	0.0017054369329422198	0.0
22	4.651191635296963E-4	4.651191635296963E-4	0.008527184664711099	0.002170556096471916	0.0
23	4.651191635296963E-4	4.651191635296963E-4	0.008527184664711099	0.0024806355388250468	0.0
24	4.651191635296963E-4	4.651191635296963E-4	0.008682224385887664	0.0029457547023547435	0.0
25	4.651191635296963E-4	4.651191635296963E-4	0.008682224385887664	0.0031007944235313086	0.0
26	4.651191635296963E-4	4.651191635296963E-4	0.008682224385887664	0.003255834144707874	0.0
27	4.651191635296963E-4	4.651191635296963E-4	0.008682224385887664	0.004186072471767267	0.0
28	4.651191635296963E-4	4.651191635296963E-4	0.008682224385887664	0.012868296857654931	0.0
29	4.651191635296963E-4	4.651191635296963E-4	0.008682224385887664	0.030697864792959956	0.0
30	4.651191635296963E-4	4.651191635296963E-4	0.008682224385887664	0.05240342575767912	0.0
31	4.651191635296963E-4	4.651191635296963E-4	0.008682224385887664	0.10914796370830207	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	450	0.0	25.077778	1
GTATCAA	1340	0.0	23.055971	1
GTCCGGC	60	3.723024E-5	21.583332	15
ACGCTAC	45	0.0038240144	20.555557	30
GACGCTA	45	0.0038240144	20.555557	29
GTCGGTA	45	0.0038240144	20.555557	20
TTCTGCG	65	6.896127E-5	19.923077	18
TTGGCGT	75	9.255435E-6	19.733334	23
TACAGTC	75	9.255435E-6	19.733334	7
GGCCGTT	285	0.0	19.473686	14
TGGCGTA	70	1.2181446E-4	18.5	24
TAGCGGA	80	1.6148777E-5	18.5	9
TTAGTAC	80	1.6148777E-5	18.5	3
CGATAAG	50	0.0070316163	18.5	12
CCCGCCA	50	0.0070316163	18.5	24
CAAGCAT	205	0.0	18.048782	37
CGGCCGT	300	0.0	17.883333	13
TAGTACT	125	8.558345E-9	17.76	4
GCCGTTA	305	0.0	17.590162	15
CCGTTAA	295	0.0	17.559324	16
>>END_MODULE