FastQCFastQC Report
Fri 10 Feb 2017
ERR1633192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607504
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30190.4969514604019068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20870.34353683267929097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11070.18222102241302116No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10580.17415523189970766No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10220.16822934499196712No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9780.16098659432695095No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8690.1430443256340699No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8390.1381060865442861No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8330.1371184387263294No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8160.13432010324211857No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7560.12444362506255104No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7020.11555479470094024No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6980.1148963628223024No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6830.11242724327741051No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6750.11111037952013486No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6560.10798282809660513No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6490.1068305723089889No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG6110.10057546946192948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA454.004167E-628.7777791
GGTATCA4450.024.5280911
GTATCAA13750.023.0072731
CCGCTGA652.6793896E-622.7692326
ATTTCGG1202.382876E-1020.04166629
CGGTTGG1306.9485395E-1018.533
CCGAGCT500.00703115818.523
TGGGGCG1306.9485395E-1018.537
TACTCGC500.00703115818.520
TCGAGCC500.00703115818.518
CCCGCTG1054.788071E-717.61904725
TTGCGGT953.6007277E-617.52631635
TGCGGTA852.7196946E-517.41176436
CCTTGCG852.7196946E-517.41176433
TCTATAC1301.3891622E-817.0769233
AATTTCG1452.9704097E-916.58620628
CTTGCGG904.440406E-516.44444534
CAACGAT803.3792827E-416.187521
GCTCGGA1851.8189894E-1116.011
CCCTCCG700.002590606715.8571439
Produced by FastQC (version 0.11.5)