##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633192.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	607504
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90623600832258	33.0	33.0	33.0	33.0	33.0
2	32.02421218625721	33.0	33.0	33.0	33.0	33.0
3	31.7329367378651	33.0	33.0	33.0	27.0	33.0
4	32.01389949695805	33.0	33.0	33.0	33.0	33.0
5	32.10757789249124	33.0	33.0	33.0	33.0	33.0
6	35.2988704601122	37.0	37.0	37.0	33.0	37.0
7	35.447127920145384	37.0	37.0	37.0	33.0	37.0
8	35.59037306750243	37.0	37.0	37.0	33.0	37.0
9	35.69127775290369	37.0	37.0	37.0	33.0	37.0
10	35.69459789565172	37.0	37.0	37.0	33.0	37.0
11	35.71589652084595	37.0	37.0	37.0	33.0	37.0
12	35.717066883510235	37.0	37.0	37.0	33.0	37.0
13	35.71850884932445	37.0	37.0	37.0	33.0	37.0
14	35.683853933472044	37.0	37.0	37.0	33.0	37.0
15	35.70639370275751	37.0	37.0	37.0	33.0	37.0
16	35.70072625036214	37.0	37.0	37.0	33.0	37.0
17	35.68400043456504	37.0	37.0	37.0	33.0	37.0
18	35.6724186178198	37.0	37.0	37.0	33.0	37.0
19	35.659045866364664	37.0	37.0	37.0	33.0	37.0
20	35.66168288603861	37.0	37.0	37.0	33.0	37.0
21	35.671154428612816	37.0	37.0	37.0	33.0	37.0
22	35.57405877162949	37.0	37.0	37.0	33.0	37.0
23	35.63177197187179	37.0	37.0	37.0	33.0	37.0
24	35.62415226895625	37.0	37.0	37.0	33.0	37.0
25	35.64749861729305	37.0	37.0	37.0	33.0	37.0
26	35.53322447259607	37.0	37.0	37.0	33.0	37.0
27	35.54484250309463	37.0	37.0	37.0	33.0	37.0
28	35.57178718164818	37.0	37.0	37.0	33.0	37.0
29	35.566816020964474	37.0	37.0	37.0	33.0	37.0
30	35.57933445705707	37.0	37.0	37.0	33.0	37.0
31	35.558292620295504	37.0	37.0	37.0	33.0	37.0
32	35.553395533198135	37.0	37.0	37.0	33.0	37.0
33	35.556862177039164	37.0	37.0	37.0	33.0	37.0
34	35.53359813268719	37.0	37.0	37.0	33.0	37.0
35	35.478307961758276	37.0	37.0	37.0	33.0	37.0
36	35.4998090547552	37.0	37.0	37.0	33.0	37.0
37	35.49340086649635	37.0	37.0	37.0	33.0	37.0
38	35.49473583713029	37.0	37.0	37.0	33.0	37.0
39	35.38775053332982	37.0	37.0	37.0	33.0	37.0
40	35.18267534040928	37.0	37.0	37.0	33.0	37.0
41	35.34248004951408	37.0	37.0	37.0	33.0	37.0
42	35.37180495930891	37.0	37.0	37.0	33.0	37.0
43	35.05291652400643	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	20.0
17	22.0
18	27.0
19	19.0
20	22.0
21	79.0
22	226.0
23	524.0
24	1073.0
25	2097.0
26	3501.0
27	5205.0
28	7813.0
29	10682.0
30	14052.0
31	18158.0
32	23473.0
33	31424.0
34	45249.0
35	95003.0
36	348821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59462982959783	21.241506228765573	13.62904606389423	24.53481787774237
2	16.04186968316258	21.444632463325345	39.03595696489241	23.477540888619664
3	17.09256235349891	27.99421896810556	30.050995549000504	24.862223129395034
4	12.04946798704206	17.99840001053491	40.70442334536069	29.247708657062336
5	13.045840027390765	37.87695225052016	35.97359029734783	13.103617424741238
6	28.529359477468464	40.95133529984988	17.518403171007925	13.000902051673735
7	25.99472596065211	32.33789407147937	24.34453106481603	17.32284890305249
8	24.06881271563644	35.97540098501409	20.7277647554584	19.228021543891067
9	25.676209539361057	15.437758434512366	20.871961336880087	38.014070689246495
10	15.05899549632595	28.00853986146593	34.08833522083805	22.844129421370067
11	32.988753983512865	24.40988042877084	24.508480602596855	18.09288498511944
12	22.81532302667966	26.538590692406967	29.81988595959862	20.826200321314754
13	26.81924728067634	22.709315494218966	26.534475493165477	23.936961731939213
14	21.61302641628697	21.17549843293213	28.304175774974322	28.907299375806577
15	23.760337380494615	28.92096183728831	25.201809370802497	22.116891411414574
16	22.590962364033818	28.68540783270563	25.48987331770655	23.233756485554004
17	21.818457162421975	28.080967368116095	26.569866206642264	23.53070926281967
18	22.198701572335324	27.857594353288206	28.292653217098156	21.651050857278307
19	24.30518976006742	26.501224683294268	27.90088624930865	21.292699307329663
20	23.20676077852985	26.894966946719695	28.214958255418892	21.683314019331558
21	22.551785667254865	27.455457083410153	28.184176565092578	21.80858068424241
22	22.277713397771866	27.162948721325293	27.86516631989254	22.694171561010297
23	22.152446732861016	27.222701414311672	28.523927414469696	22.10092443835761
24	22.472773841818324	28.022366930917325	26.742704574784693	22.762154652479655
25	22.435243224735967	27.307639126655957	28.20327108957307	22.053846559035005
26	22.499275724933497	27.657924886091283	28.2612130948932	21.581586294082015
27	22.48561326345176	27.501218098975478	27.091838078432406	22.92133055914035
28	21.660433511548895	27.025994890568622	29.03437672838368	22.279194869498802
29	23.923463878427135	26.78221048750296	27.503028786641735	21.791296847428164
30	23.065691748531698	26.66385735731781	28.070597066027545	22.199853828122944
31	22.394420448260423	27.056941188864602	28.26187152677184	22.286766836103137
32	21.4890766151334	26.94698306513208	28.35685032526535	23.20708999446917
33	21.55277989939161	27.127393399878848	28.79223840501462	22.527588295714924
34	22.658780847533514	26.872909478785324	28.54236350707156	21.925946166609602
35	22.569563327978088	27.56311069556744	27.777430272064052	22.089895704390425
36	23.42980457741842	27.35438779003924	27.066159235165532	22.149648397376804
37	22.3218283336406	26.66402196528747	27.72393268192473	23.2902170191472
38	22.860425610366352	27.156693618478233	27.477679159314178	22.505201611841237
39	21.900267323342725	27.29183676156865	28.111584450472755	22.69631146461587
40	22.26750770365298	27.852326898259104	28.261048486923546	21.619116911164372
41	22.01796202164924	26.694145223735156	28.123600832257896	23.164291922357712
42	21.595413363533407	27.922943717243015	27.959157470568098	22.522485448655484
43	22.740920224393584	26.152585003555533	28.154053306644894	22.95244146540599
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	44.0
2	61.0
3	207.5
4	354.0
5	354.0
6	405.5
7	457.0
8	426.0
9	395.0
10	544.5
11	694.0
12	694.0
13	1155.0
14	1616.0
15	2764.0
16	3912.0
17	4565.5
18	5219.0
19	5219.0
20	4778.5
21	4338.0
22	4833.0
23	5328.0
24	6792.0
25	8256.0
26	8256.0
27	10632.5
28	13009.0
29	15281.0
30	17553.0
31	21033.5
32	24514.0
33	24514.0
34	30072.5
35	35631.0
36	38962.0
37	42293.0
38	45016.5
39	47740.0
40	47740.0
41	49496.5
42	51253.0
43	51768.5
44	52284.0
45	50299.0
46	48314.0
47	48314.0
48	45448.0
49	42582.0
50	42480.5
51	42379.0
52	39914.5
53	37450.0
54	37450.0
55	35935.5
56	34421.0
57	29193.0
58	23965.0
59	21618.0
60	19271.0
61	19271.0
62	17264.5
63	15258.0
64	12150.5
65	9043.0
66	7484.5
67	5926.0
68	5926.0
69	4981.0
70	4036.0
71	3532.5
72	3029.0
73	3020.0
74	3011.0
75	3011.0
76	2622.5
77	2234.0
78	1598.0
79	962.0
80	625.5
81	289.0
82	289.0
83	230.0
84	171.0
85	148.0
86	125.0
87	95.5
88	66.0
89	66.0
90	48.5
91	31.0
92	18.5
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	607504.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.545613535955894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.20257674563643	45.82112897015746
2	6.925109056642002	7.277682083693174
3	1.8068859737423948	2.8483179623942165
4	0.8651680373455958	1.8184314133609252
5	0.5183392349046548	1.3618226558911528
6	0.3440198969836447	1.084604193334917
7	0.27222495654973644	1.0012959153193386
8	0.21391393965098765	0.8992191362283672
9	0.168290768111932	0.7958647496590872
>10	1.3627057009872923	14.317714507141833
>50	0.19188661860858108	7.063645216052923
>100	0.11933247299708914	11.580505463130883
>500	0.007955498199805938	2.743090115405837
>1k	0.0015910996399611875	1.3866776182298453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3019	0.4969514604019068	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2087	0.34353683267929097	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1107	0.18222102241302116	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1058	0.17415523189970766	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1022	0.16822934499196712	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	978	0.16098659432695095	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	869	0.1430443256340699	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	839	0.1381060865442861	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	833	0.1371184387263294	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	816	0.13432010324211857	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	756	0.12444362506255104	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	702	0.11555479470094024	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	698	0.1148963628223024	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	683	0.11242724327741051	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	675	0.11111037952013486	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	656	0.10798282809660513	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	649	0.1068305723089889	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	611	0.10057546946192948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.6460796965945903E-4	0.0	0.0
2	0.0	0.0	3.2921593931891806E-4	1.6460796965945903E-4	0.0
3	0.0	0.0	3.2921593931891806E-4	1.6460796965945903E-4	0.0
4	0.0	0.0	4.938239089783771E-4	3.2921593931891806E-4	0.0
5	0.0	0.0	6.584318786378361E-4	6.584318786378361E-4	0.0
6	0.0	0.0	0.0019752956359135082	6.584318786378361E-4	0.0
7	0.0	0.0	0.004609023150464853	6.584318786378361E-4	0.0
8	1.6460796965945903E-4	0.0	0.005432062998762148	8.230398482972952E-4	0.0
9	1.6460796965945903E-4	0.0	0.006584318786378361	9.876478179567541E-4	0.0
10	3.2921593931891806E-4	0.0	0.007571966604335115	9.876478179567541E-4	0.0
11	3.2921593931891806E-4	0.0	0.010699518027864837	0.0013168637572756722	0.0
12	3.2921593931891806E-4	0.0	0.011193341936843215	0.0013168637572756722	0.0
13	3.2921593931891806E-4	0.0	0.011687165845821591	0.0014814717269351313	0.0
14	3.2921593931891806E-4	0.0	0.01201638178514051	0.0014814717269351313	0.0
15	3.2921593931891806E-4	0.0	0.012180989754799969	0.0016460796965945903	0.0
16	3.2921593931891806E-4	0.0	0.013004029603097263	0.0019752956359135082	0.0
17	3.2921593931891806E-4	0.0	0.013168637572756723	0.0019752956359135082	0.0
18	3.2921593931891806E-4	0.0	0.013333245542416182	0.0019752956359135082	0.0
19	3.2921593931891806E-4	0.0	0.013497853512075641	0.0019752956359135082	0.0
20	3.2921593931891806E-4	0.0	0.013827069451394558	0.0023045115752324263	0.0
21	3.2921593931891806E-4	0.0	0.014485501330032395	0.0027983354842108037	0.0
22	3.2921593931891806E-4	0.0	0.014485501330032395	0.0032921593931891807	0.0
23	3.2921593931891806E-4	0.0	0.014485501330032395	0.0034567673628486395	0.0
24	3.2921593931891806E-4	0.0	0.014485501330032395	0.004279807211145935	0.0
25	3.2921593931891806E-4	0.0	0.014485501330032395	0.004773631120124312	0.0
26	3.2921593931891806E-4	0.0	0.014650109299691854	0.00510284705944323	0.0
27	3.2921593931891806E-4	0.0	0.014650109299691854	0.005596670968421607	0.0
28	3.2921593931891806E-4	0.0	0.014650109299691854	0.01876530854117833	0.0
29	3.2921593931891806E-4	0.0	0.014650109299691854	0.041481208354183674	0.0
30	3.2921593931891806E-4	0.0	0.014650109299691854	0.07045221101424846	0.0
31	3.2921593931891806E-4	0.0	0.014650109299691854	0.13596618293871315	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTATA	45	4.004167E-6	28.777779	1
GGTATCA	445	0.0	24.528091	1
GTATCAA	1375	0.0	23.007273	1
CCGCTGA	65	2.6793896E-6	22.76923	26
ATTTCGG	120	2.382876E-10	20.041666	29
CGGTTGG	130	6.9485395E-10	18.5	33
CCGAGCT	50	0.007031158	18.5	23
TGGGGCG	130	6.9485395E-10	18.5	37
TACTCGC	50	0.007031158	18.5	20
TCGAGCC	50	0.007031158	18.5	18
CCCGCTG	105	4.788071E-7	17.619047	25
TTGCGGT	95	3.6007277E-6	17.526316	35
TGCGGTA	85	2.7196946E-5	17.411764	36
CCTTGCG	85	2.7196946E-5	17.411764	33
TCTATAC	130	1.3891622E-8	17.076923	3
AATTTCG	145	2.9704097E-9	16.586206	28
CTTGCGG	90	4.440406E-5	16.444445	34
CAACGAT	80	3.3792827E-4	16.1875	21
GCTCGGA	185	1.8189894E-11	16.0	11
CCCTCCG	70	0.0025906067	15.857143	9
>>END_MODULE