FastQCFastQC Report
Fri 10 Feb 2017
ERR1633191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739080
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57620.7796179033392867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44490.601964604643611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21110.2856253720842128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.14815716837148887No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9040.12231422850029768No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA8350.11297829734264221No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG8240.11148996049142176No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7840.10607782648698381No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7580.10255993938409916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8050.028.2670821
CCGGTCG358.866388E-426.428579
GTATCAA22250.023.4471911
GCCGCTC1800.022.6111127
TCGGGAT502.700837E-422.222
CCGCTCT1850.022.028
GCCGGCA2050.021.65853715
TTCGCCG1900.021.42105324
CGCTCTC1950.020.87179429
GCTTCGC2050.020.756122
CGGGTAT450.00382453520.55555517
CGGTCGT450.00382453520.55555510
CCGTTAA656.897868E-519.92307716
AGCTTCG2050.019.8536621
CCGGCAG2350.019.68085116
GCGGTAA951.6740887E-719.47368423
ATTAGAC1052.2560926E-819.380953
TGCTCGC2250.018.9111110
CGCCGGC2450.018.8775514
CTGCTCG2750.018.8363639
Produced by FastQC (version 0.11.5)