##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	739080
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936856632570223	33.0	33.0	33.0	33.0	33.0
2	32.03442523136873	33.0	33.0	33.0	33.0	33.0
3	31.757553986036694	33.0	33.0	33.0	27.0	33.0
4	32.02850300373437	33.0	33.0	33.0	33.0	33.0
5	32.12724332954484	33.0	33.0	33.0	33.0	33.0
6	35.3145085782324	37.0	37.0	37.0	33.0	37.0
7	35.45608188558749	37.0	37.0	37.0	33.0	37.0
8	35.60066975158305	37.0	37.0	37.0	33.0	37.0
9	35.71994641987336	37.0	37.0	37.0	33.0	37.0
10	35.718984413054066	37.0	37.0	37.0	33.0	37.0
11	35.725148833685125	37.0	37.0	37.0	33.0	37.0
12	35.724082643286245	37.0	37.0	37.0	33.0	37.0
13	35.727029550251665	37.0	37.0	37.0	33.0	37.0
14	35.70509417113168	37.0	37.0	37.0	33.0	37.0
15	35.73066785733615	37.0	37.0	37.0	33.0	37.0
16	35.71111652324512	37.0	37.0	37.0	33.0	37.0
17	35.6999986469665	37.0	37.0	37.0	33.0	37.0
18	35.68738566866916	37.0	37.0	37.0	33.0	37.0
19	35.67546544352438	37.0	37.0	37.0	33.0	37.0
20	35.68709070736591	37.0	37.0	37.0	33.0	37.0
21	35.68068138767116	37.0	37.0	37.0	33.0	37.0
22	35.58369594631163	37.0	37.0	37.0	33.0	37.0
23	35.62460491421768	37.0	37.0	37.0	33.0	37.0
24	35.62215457054717	37.0	37.0	37.0	33.0	37.0
25	35.638940304161935	37.0	37.0	37.0	33.0	37.0
26	35.53543188829355	37.0	37.0	37.0	33.0	37.0
27	35.54866455593441	37.0	37.0	37.0	33.0	37.0
28	35.56593061644206	37.0	37.0	37.0	33.0	37.0
29	35.57197461709152	37.0	37.0	37.0	33.0	37.0
30	35.576492395951725	37.0	37.0	37.0	33.0	37.0
31	35.56653542241706	37.0	37.0	37.0	33.0	37.0
32	35.552898197759376	37.0	37.0	37.0	33.0	37.0
33	35.541251285381826	37.0	37.0	37.0	33.0	37.0
34	35.51516750554744	37.0	37.0	37.0	33.0	37.0
35	35.4418330897873	37.0	37.0	37.0	33.0	37.0
36	35.4625575039238	37.0	37.0	37.0	33.0	37.0
37	35.44986469664989	37.0	37.0	37.0	33.0	37.0
38	35.43181387671159	37.0	37.0	37.0	33.0	37.0
39	35.327313687286896	37.0	37.0	37.0	33.0	37.0
40	35.10824944525626	37.0	37.0	37.0	33.0	37.0
41	35.25873247821616	37.0	37.0	37.0	33.0	37.0
42	35.28969800292255	37.0	37.0	37.0	33.0	37.0
43	34.96481436380365	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	19.0
16	34.0
17	30.0
18	32.0
19	22.0
20	40.0
21	107.0
22	289.0
23	648.0
24	1390.0
25	2520.0
26	4330.0
27	6621.0
28	9366.0
29	12812.0
30	17438.0
31	22257.0
32	28877.0
33	38076.0
34	55418.0
35	113469.0
36	425281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61849867402717	21.12775342317476	14.132299615738486	23.121448287059586
2	15.504004979163282	22.94812469556746	39.62385668669156	21.92401363857769
3	17.60431888293554	28.09100503328462	30.795177788602047	23.50949829517779
4	11.781945120961195	18.49677978026736	40.97093684039616	28.75033825837528
5	13.429128105211884	37.38837473615847	35.30984467175407	13.872652486875575
6	27.63679168696217	41.34965091735671	17.985197813497862	13.028359582183255
7	25.42336418249716	33.212642745034366	23.808248092222765	17.55574498024571
8	24.084672836499433	35.553661308654	21.368728689722357	18.992937165124207
9	25.826568165827783	15.197948801212318	21.141554364886076	37.833928668073824
10	14.51250202955025	28.60705201060778	34.43592033338746	22.44452562645451
11	32.98167992639498	24.795286031282135	24.68758456459382	17.535449477729067
12	22.2960978513828	26.27915787194891	31.514450397791848	19.91029387887644
13	28.12618390431347	23.07490393462142	26.90953618011582	21.88937598094929
14	21.672620014071548	21.885587487146182	29.221329220111492	27.22046327867078
15	22.943794988363912	29.474211181468853	25.92642203820967	21.65557179195757
16	21.785733614764304	29.23513016182281	26.152513936245057	22.82662228716783
17	21.676408507874655	27.756129241760025	27.2664664177085	23.300995832656817
18	22.525437029820857	26.697515830491962	29.227011960816153	21.55003517887103
19	24.20428099799751	26.80467608377983	28.980083346863665	20.010959571358985
20	23.96492937165124	26.601044541862855	28.728554419007413	20.705471667478488
21	22.35725496563295	27.39324565676246	28.79187638685934	21.45762299074525
22	22.389321859609243	26.8153650484386	29.0830491962981	21.712263895654058
23	21.623369594631164	26.99491259403583	29.637792931752987	21.743924879580018
24	21.728635601017483	27.505682740704664	28.83990907614872	21.925772582129134
25	21.977187855171294	27.56453969800292	29.425772582129134	21.03249986469665
26	22.489446338691348	27.18027818368783	28.676192022514478	21.65408345510635
27	22.329517778860204	27.21816312171889	28.5281701574931	21.9241489419278
28	21.842561021810898	27.097607836770038	29.070601288087893	21.989229853331167
29	22.446690480056287	27.360908156085944	28.707582399740218	21.48481896411755
30	22.543567678735727	27.16769497212751	28.778481355198355	21.510255993938408
31	22.202197326405802	26.77274449315365	28.866563836120584	22.158494344319966
32	21.257238729230934	27.165936028576066	29.22444119716404	22.352384045028955
33	21.569924771337337	26.952968555501432	29.35379119987011	22.123315473291118
34	21.876522162688747	27.045380743627213	29.13297613248904	21.945120961195
35	22.013043242950694	27.405693564972665	28.625588569572987	21.955674622503654
36	22.124262596741893	27.13359852789955	28.57214374627916	22.169995129079396
37	22.315716837148887	27.029009038263784	28.91784380581263	21.737430318774692
38	21.919007414623586	27.101125723872922	28.405314715592354	22.574552145911134
39	21.83376630405369	26.853249986469663	28.924203063267846	22.3887806462088
40	21.939844130540674	27.354684201980838	28.912161065107973	21.793310602370514
41	22.1032905774747	26.966363587162416	28.694593278129567	22.23575255723332
42	21.27103967094225	28.063944363262433	28.57809709368404	22.086918872111276
43	22.10383179087514	26.839178438058127	28.476484277750718	22.580505493316014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	81.5
2	122.0
3	357.5
4	593.0
5	593.0
6	772.5
7	952.0
8	909.5
9	867.0
10	1174.5
11	1482.0
12	1482.0
13	2369.5
14	3257.0
15	5727.5
16	8198.0
17	9379.0
18	10560.0
19	10560.0
20	9900.0
21	9240.0
22	10226.5
23	11213.0
24	13700.5
25	16188.0
26	16188.0
27	19049.0
28	21910.0
29	24879.0
30	27848.0
31	30380.0
32	32912.0
33	32912.0
34	37721.5
35	42531.0
36	44121.0
37	45711.0
38	46625.5
39	47540.0
40	47540.0
41	48494.5
42	49449.0
43	50127.0
44	50805.0
45	50746.0
46	50687.0
47	50687.0
48	49942.0
49	49197.0
50	49486.5
51	49776.0
52	49887.0
53	49998.0
54	49998.0
55	46583.5
56	43169.0
57	37777.5
58	32386.0
59	29578.5
60	26771.0
61	26771.0
62	23492.5
63	20214.0
64	15749.0
65	11284.0
66	9798.0
67	8312.0
68	8312.0
69	6936.0
70	5560.0
71	5113.0
72	4666.0
73	4077.0
74	3488.0
75	3488.0
76	2190.0
77	892.0
78	705.0
79	518.0
80	404.5
81	291.0
82	291.0
83	240.5
84	190.0
85	158.0
86	126.0
87	105.5
88	85.0
89	85.0
90	62.0
91	39.0
92	24.5
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	739080.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.04594589969582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.78728610501689	57.752530085677435
2	7.235285440709556	9.412519706905014
3	1.4720654694725788	2.8725567266437113
4	0.6360049934750261	1.6547818559005179
5	0.34602373536828374	1.1253720585388025
6	0.24284496377090986	0.9477648225273718
7	0.16577389500225737	0.7548043864129081
8	0.12565112438508322	0.6538476991190459
9	0.09821906931436761	0.5749877042046746
>10	0.7393957918003574	9.587522847214865
>50	0.08276182336215937	3.7088837919665045
>100	0.06448651258475137	7.677732339416934
>500	0.0033608605902821645	1.443511507833541
>1k	6.301613606779058E-4	1.0459139226186234
>5k	2.1005378689263528E-4	0.7872705450200532
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5762	0.7796179033392867	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4449	0.601964604643611	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2111	0.2856253720842128	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1095	0.14815716837148887	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	904	0.12231422850029768	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	835	0.11297829734264221	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	824	0.11148996049142176	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	784	0.10607782648698381	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	758	0.10255993938409916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.0591005033284623E-4	0.0	0.0
2	1.3530335011094875E-4	0.0	5.41213400443795E-4	0.0	0.0
3	1.3530335011094875E-4	0.0	8.118201006656925E-4	0.0	0.0
4	1.3530335011094875E-4	0.0	0.00108242680088759	0.0	0.0
5	1.3530335011094875E-4	0.0	0.0014883368512204362	0.0	0.0
6	1.3530335011094875E-4	0.0	0.0028413703523299237	0.0	0.0
7	1.3530335011094875E-4	0.0	0.006223954105103642	1.3530335011094875E-4	0.0
8	1.3530335011094875E-4	0.0	0.007171077555880283	1.3530335011094875E-4	0.0
9	1.3530335011094875E-4	0.0	0.00974184120798831	6.765167505547437E-4	0.0
10	4.0591005033284623E-4	0.0	0.01190669480976349	9.471234507766412E-4	0.0
11	4.0591005033284623E-4	0.0	0.01840125561508903	9.471234507766412E-4	0.0
12	4.0591005033284623E-4	0.0	0.020295502516642312	0.00108242680088759	0.0
13	4.0591005033284623E-4	0.0	0.022189749418195594	0.0013530335011094875	0.0
14	5.41213400443795E-4	0.0	0.02273096281863939	0.0013530335011094875	0.0
15	5.41213400443795E-4	0.0	0.024219299669859827	0.0014883368512204362	0.0
16	5.41213400443795E-4	0.0	0.02611354657141311	0.0014883368512204362	0.0
17	5.41213400443795E-4	0.0	0.0269253666720788	0.0017589435514423336	0.0
18	5.41213400443795E-4	0.0	0.027601883422633543	0.0017589435514423336	0.0
19	5.41213400443795E-4	0.0	0.02814309682307734	0.0018942469015532824	0.0
20	5.41213400443795E-4	0.0	0.028819613573632084	0.0023001569518861288	0.0
21	5.41213400443795E-4	0.0	0.029902040374519673	0.0024354603019970775	0.0
22	5.41213400443795E-4	0.0	0.03044325377496347	0.0028413703523299237	0.0
23	5.41213400443795E-4	0.0	0.030713860475185366	0.0037884938031065647	0.0
24	5.41213400443795E-4	0.0	0.030713860475185366	0.0046003139037722575	0.0
25	6.765167505547437E-4	0.0	0.030849163825296313	0.0060886507549926935	0.0
26	6.765167505547437E-4	0.0	0.030849163825296313	0.006629864155436488	0.0
27	8.118201006656925E-4	0.0	0.030984467175407263	0.008118201006656925	0.0
28	8.118201006656925E-4	0.0	0.030984467175407263	0.023272176219083184	0.0
29	8.118201006656925E-4	0.0	0.030984467175407263	0.0543919467446014	0.0
30	8.118201006656925E-4	0.0	0.03125507387562916	0.08862369432267143	0.0
31	8.118201006656925E-4	0.0	0.03125507387562916	0.16263462683336039	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	805	0.0	28.267082	1
CCGGTCG	35	8.866388E-4	26.42857	9
GTATCAA	2225	0.0	23.447191	1
GCCGCTC	180	0.0	22.61111	27
TCGGGAT	50	2.700837E-4	22.2	22
CCGCTCT	185	0.0	22.0	28
GCCGGCA	205	0.0	21.658537	15
TTCGCCG	190	0.0	21.421053	24
CGCTCTC	195	0.0	20.871794	29
GCTTCGC	205	0.0	20.7561	22
CGGGTAT	45	0.003824535	20.555555	17
CGGTCGT	45	0.003824535	20.555555	10
CCGTTAA	65	6.897868E-5	19.923077	16
AGCTTCG	205	0.0	19.85366	21
CCGGCAG	235	0.0	19.680851	16
GCGGTAA	95	1.6740887E-7	19.473684	23
ATTAGAC	105	2.2560926E-8	19.38095	3
TGCTCGC	225	0.0	18.91111	10
CGCCGGC	245	0.0	18.87755	14
CTGCTCG	275	0.0	18.836363	9
>>END_MODULE