FastQCFastQC Report
Fri 10 Feb 2017
ERR1633190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667973
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32000.47906127942297067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.33279788254914494No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC17000.25450130469345317No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15670.23459032026743593No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14090.21093666959592677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13110.19626541791359828No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12770.19117539181972926No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11910.1783006199352369No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11270.1687193943467775No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10030.1501557697691374No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9620.14401779712653057No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9130.13668217128536633No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8870.1327897983900547No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8860.13264009174023503No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8540.12784947894600532No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8140.12186121295321817No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8060.12066355975466074No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7940.1188670799568246No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7930.11871737330700492No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7840.11737001345862783No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7710.11542382701097201No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7670.11482500041169329No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7410.11093262751638165No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7350.11003438761746358No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7040.10539348147305355No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6920.1035970016752174No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6840.10239934847665999No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCAT250.00549471729.59999819
GGTATCA5750.028.3130441
GGCCGTC358.86549E-426.4285719
TGGCGTA601.3360059E-624.66666824
GTATCAA16800.023.014881
CCGCTCT855.1724783E-821.76470628
GCCGCTC957.1322575E-921.42105327
GCCGGCA909.4632924E-820.55555515
GTCCGTA450.003824156220.5555559
CCGGTCG555.139994E-420.1818189
TTTGGCG759.256246E-619.73333422
CGAGCTC902.1494707E-618.528
CGCCGCT1103.844434E-818.526
TGGTCTA801.6150192E-518.535
CCACGTT500.007031874318.49999837
ATCGAGC1002.870438E-718.49999826
CCGCAAG1850.018.09
CATGCTA3500.017.971434
AGCTTCG1054.7898357E-717.61904721
CGCTCTC1054.7898357E-717.61904729
Produced by FastQC (version 0.11.5)