##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633190.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	667973
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.805393331766403	33.0	33.0	33.0	27.0	33.0
2	31.927821932922438	33.0	33.0	33.0	33.0	33.0
3	31.656977153268173	33.0	33.0	33.0	27.0	33.0
4	31.939365812690035	33.0	33.0	33.0	33.0	33.0
5	32.0422606901776	33.0	33.0	33.0	33.0	33.0
6	35.18971874611698	37.0	37.0	37.0	33.0	37.0
7	35.34796017204288	37.0	37.0	37.0	33.0	37.0
8	35.466140098477034	37.0	37.0	37.0	33.0	37.0
9	35.5982562169429	37.0	37.0	37.0	33.0	37.0
10	35.59373956731784	37.0	37.0	37.0	33.0	37.0
11	35.60809493796905	37.0	37.0	37.0	33.0	37.0
12	35.58969898483921	37.0	37.0	37.0	33.0	37.0
13	35.60222194609663	37.0	37.0	37.0	33.0	37.0
14	35.59166463315134	37.0	37.0	37.0	33.0	37.0
15	35.59197901711596	37.0	37.0	37.0	33.0	37.0
16	35.58266426936418	37.0	37.0	37.0	33.0	37.0
17	35.57379415036237	37.0	37.0	37.0	33.0	37.0
18	35.56498391402048	37.0	37.0	37.0	33.0	37.0
19	35.565971977909285	37.0	37.0	37.0	33.0	37.0
20	35.55512573112985	37.0	37.0	37.0	33.0	37.0
21	35.55578144625606	37.0	37.0	37.0	33.0	37.0
22	35.46340046678534	37.0	37.0	37.0	33.0	37.0
23	35.51413904454222	37.0	37.0	37.0	33.0	37.0
24	35.51564060223991	37.0	37.0	37.0	33.0	37.0
25	35.52811865150238	37.0	37.0	37.0	33.0	37.0
26	35.421555961094235	37.0	37.0	37.0	33.0	37.0
27	35.432542932124505	37.0	37.0	37.0	33.0	37.0
28	35.45754693677738	37.0	37.0	37.0	33.0	37.0
29	35.460458731116375	37.0	37.0	37.0	33.0	37.0
30	35.46391845179371	37.0	37.0	37.0	33.0	37.0
31	35.45362462255211	37.0	37.0	37.0	33.0	37.0
32	35.442303805692745	37.0	37.0	37.0	33.0	37.0
33	35.44338019650495	37.0	37.0	37.0	33.0	37.0
34	35.42314734278182	37.0	37.0	37.0	33.0	37.0
35	35.3519273982631	37.0	37.0	37.0	33.0	37.0
36	35.38423409329419	37.0	37.0	37.0	33.0	37.0
37	35.382006458344875	37.0	37.0	37.0	33.0	37.0
38	35.35971663525322	37.0	37.0	37.0	33.0	37.0
39	35.27460840483074	37.0	37.0	37.0	33.0	37.0
40	35.0662541749442	37.0	37.0	37.0	33.0	37.0
41	35.24014443697575	37.0	37.0	37.0	33.0	37.0
42	35.281250888883235	37.0	37.0	37.0	33.0	37.0
43	34.949951569898786	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	28.0
16	38.0
17	41.0
18	20.0
19	36.0
20	47.0
21	132.0
22	304.0
23	752.0
24	1502.0
25	2791.0
26	4466.0
27	6656.0
28	9599.0
29	12818.0
30	16687.0
31	21379.0
32	27191.0
33	35638.0
34	51616.0
35	104516.0
36	371710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55976364314126	20.454419564862654	13.368953535547096	24.616863256448987
2	16.49003777098775	21.018963341332658	39.054722271708584	23.436276615971003
3	17.466574247761514	27.62866163752128	29.737130093581627	25.167634021135587
4	12.331636158946544	17.630952149263518	40.154916441233404	29.882495250556534
5	13.4039848916049	37.57322526509305	35.637218869624974	13.38557097367708
6	29.47170020345134	40.177222732056535	17.03032907018697	13.32074799430516
7	26.83237196713041	32.01371911738948	23.756648846585115	17.397260068895
8	24.478085192066146	35.97720267136546	20.163838957562653	19.38087317900574
9	26.186986599757773	15.113634832545628	20.22372161749053	38.47565695020607
10	15.572785127542579	27.717737094163986	33.741932682907844	22.967545095385592
11	33.89388493247482	23.8295559850473	23.700059732953278	18.576499349524607
12	23.60559483691706	26.09686319656633	29.304328168952935	20.993213797563673
13	27.222806909860132	22.26122912153635	26.110187088400277	24.40577688020324
14	22.062418690575818	20.6936507912745	27.845137453160536	29.398793064989153
15	24.469551913026425	28.175090909363103	25.02122091761194	22.334136259998534
16	23.0299727683604	28.128232727969543	24.994722840593855	23.847071663076203
17	22.337579512944387	27.534346448134876	26.119468900689103	24.008605138231633
18	22.761399038583896	27.156486863990008	28.043648470821424	22.038465626604665
19	25.199970657496635	25.73891459684748	27.17579902181675	21.88531572383914
20	23.940488612563684	26.21393379672532	27.883761768814008	21.961815821896995
21	23.40618557935725	26.781771119491353	27.51174074401211	22.300302557139283
22	22.821880525111045	26.884769294567295	27.341374576517314	22.951975603804346
23	22.844785642533456	26.846294685563638	27.988706130337604	22.320213541565302
24	23.35962681126333	27.512189863961567	26.0434179225807	23.0847654021944
25	23.06200999142181	26.790903225130357	27.786901566380678	22.36018521706716
26	23.291061165645914	27.104838069802224	27.836155054171353	21.76794571038051
27	23.28387524645457	26.751380669577962	26.576373595938758	23.38837048802871
28	22.236078404366644	26.470530994516245	28.592622755710185	22.700767845406926
29	24.567460062008493	26.08638373107895	27.072651140090993	22.273505066821564
30	23.5587366555235	26.068718346400228	27.848430999456564	22.524113998619704
31	22.87951758529162	26.56140293095679	27.927476110561354	22.631603373190234
32	21.78531168175959	26.27636146970012	28.18122888200571	23.757097966534577
33	22.090713247391736	26.647783667902747	28.318509879890353	22.942993204815167
34	23.264263675328195	26.408702148140716	28.160120244381137	22.16691393214995
35	23.34974617237523	26.98147979035081	27.289126955730246	22.379647081543716
36	24.21085882213802	26.7600636552675	26.58969748777271	22.439380034821767
37	22.816940205666995	26.23070094150512	27.344967536112986	23.6073913167149
38	23.545712176989188	26.70976222092809	27.03582330423535	22.70870229784737
39	22.375305588698943	26.804376823614128	27.66788477977403	23.152432807912895
40	22.951526483854888	27.154690384192175	27.866695210734566	22.02708792121837
41	22.446266540713474	25.94625830684773	28.01176095440983	23.59571419802896
42	22.058526317680503	27.498716265477796	27.49317711943447	22.94958029740723
43	23.56891670771124	25.465250840977106	27.47835616110232	23.487476290209337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	45.5
2	52.0
3	189.0
4	326.0
5	326.0
6	413.0
7	500.0
8	451.0
9	402.0
10	551.0
11	700.0
12	700.0
13	1121.0
14	1542.0
15	2785.0
16	4028.0
17	4569.5
18	5111.0
19	5111.0
20	4678.5
21	4246.0
22	4402.0
23	4558.0
24	5950.5
25	7343.0
26	7343.0
27	9579.0
28	11815.0
29	14548.5
30	17282.0
31	20846.0
32	24410.0
33	24410.0
34	30669.5
35	36929.0
36	40377.0
37	43825.0
38	47427.5
39	51030.0
40	51030.0
41	53113.0
42	55196.0
43	56139.5
44	57083.0
45	54848.0
46	52613.0
47	52613.0
48	49261.0
49	45909.0
50	46616.0
51	47323.0
52	44565.0
53	41807.0
54	41807.0
55	42235.0
56	42663.0
57	35927.5
58	29192.0
59	26407.0
60	23622.0
61	23622.0
62	21577.5
63	19533.0
64	15472.5
65	11412.0
66	9384.5
67	7357.0
68	7357.0
69	6257.0
70	5157.0
71	4610.0
72	4063.0
73	4449.0
74	4835.0
75	4835.0
76	4197.5
77	3560.0
78	2580.0
79	1600.0
80	981.0
81	362.0
82	362.0
83	289.5
84	217.0
85	202.5
86	188.0
87	143.0
88	98.0
89	98.0
90	68.5
91	39.0
92	22.5
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	667973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.72255860379772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.57010744726844	41.313470259893336
2	7.2333841371808	6.903911967807829
3	1.8837266696501493	2.6968876915774787
4	0.8864934547786782	1.6922294339023431
5	0.5421051399283591	1.2935322154825541
6	0.3626507819047434	1.0383973915297309
7	0.28925146461846885	0.9662673980042441
8	0.2228117596266854	0.8506517805119829
9	0.18522647880685217	0.7955533340851589
>10	1.4336081687793247	13.971344995725701
>50	0.20457247894801353	6.8934565433662405
>100	0.1704238728715098	15.147297256917488
>500	0.012446687344548464	3.9989084076821992
>1k	0.003191458293473965	2.4380913235137363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3200	0.47906127942297067	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2223	0.33279788254914494	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1700	0.25450130469345317	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1567	0.23459032026743593	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1409	0.21093666959592677	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1311	0.19626541791359828	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1277	0.19117539181972926	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1191	0.1783006199352369	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1127	0.1687193943467775	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1003	0.1501557697691374	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	962	0.14401779712653057	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	913	0.13668217128536633	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	887	0.1327897983900547	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	886	0.13264009174023503	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	854	0.12784947894600532	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	814	0.12186121295321817	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	806	0.12066355975466074	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	794	0.1188670799568246	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	793	0.11871737330700492	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	784	0.11737001345862783	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	771	0.11542382701097201	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	767	0.11482500041169329	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	741	0.11093262751638165	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	735	0.11003438761746358	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	704	0.10539348147305355	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	692	0.1035970016752174	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	684	0.10239934847665999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.4970664981967834E-4	0.0	0.0
2	0.0	0.0	1.4970664981967834E-4	0.0	0.0
3	0.0	0.0	1.4970664981967834E-4	0.0	0.0
4	0.0	0.0	1.4970664981967834E-4	0.0	0.0
5	0.0	0.0	2.994132996393567E-4	0.0	0.0
6	0.0	0.0	7.485332490983917E-4	0.0	0.0
7	1.4970664981967834E-4	0.0	0.0020958930974754966	1.4970664981967834E-4	0.0
8	2.994132996393567E-4	0.0	0.0028444263465738887	1.4970664981967834E-4	0.0
9	2.994132996393567E-4	0.0	0.004640906144410028	0.0010479465487377483	0.0
10	4.4911994945903504E-4	0.0	0.004790612794229707	0.0011976531985574267	0.0
11	4.4911994945903504E-4	0.0	0.006886505891705204	0.0013473598483771051	0.0
12	4.4911994945903504E-4	0.0	0.007335625841164239	0.0013473598483771051	0.0
13	4.4911994945903504E-4	0.0	0.007485332490983917	0.0013473598483771051	0.0
14	4.4911994945903504E-4	0.0	0.007934452440442953	0.0013473598483771051	0.0
15	4.4911994945903504E-4	0.0	0.008682985689541343	0.0013473598483771051	0.0
16	4.4911994945903504E-4	0.0	0.009431518938639735	0.0014970664981967833	0.0
17	4.4911994945903504E-4	0.0	0.010030345537918449	0.0014970664981967833	0.0
18	4.4911994945903504E-4	0.0	0.010180052187738127	0.0014970664981967833	0.0
19	4.4911994945903504E-4	0.0	0.010778878787016841	0.0016467731480164617	0.0
20	4.4911994945903504E-4	0.0	0.010778878787016841	0.0020958930974754966	0.0
21	4.4911994945903504E-4	0.0	0.011377705386295555	0.0023953063971148534	0.0
22	4.4911994945903504E-4	0.0	0.011377705386295555	0.002545013046934532	0.0
23	4.4911994945903504E-4	0.0	0.011377705386295555	0.0028444263465738887	0.0
24	4.4911994945903504E-4	0.0	0.011527412036115231	0.004042079545131316	0.0
25	4.4911994945903504E-4	0.0	0.01167711868593491	0.00449119949459035	0.0
26	4.4911994945903504E-4	0.0	0.01167711868593491	0.005090026093869064	0.0
27	5.988265992787134E-4	0.0	0.01167711868593491	0.00628767929242649	0.0
28	5.988265992787134E-4	0.0	0.01167711868593491	0.014072425083049764	0.0
29	5.988265992787134E-4	0.0	0.01167711868593491	0.030989276512673417	0.0
30	5.988265992787134E-4	0.0	0.01167711868593491	0.055690873732920344	0.0
31	5.988265992787134E-4	0.0	0.011826825335754588	0.11242969401457843	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGCAT	25	0.005494717	29.599998	19
GGTATCA	575	0.0	28.313044	1
GGCCGTC	35	8.86549E-4	26.42857	19
TGGCGTA	60	1.3360059E-6	24.666668	24
GTATCAA	1680	0.0	23.01488	1
CCGCTCT	85	5.1724783E-8	21.764706	28
GCCGCTC	95	7.1322575E-9	21.421053	27
GCCGGCA	90	9.4632924E-8	20.555555	15
GTCCGTA	45	0.0038241562	20.555555	9
CCGGTCG	55	5.139994E-4	20.181818	9
TTTGGCG	75	9.256246E-6	19.733334	22
CGAGCTC	90	2.1494707E-6	18.5	28
CGCCGCT	110	3.844434E-8	18.5	26
TGGTCTA	80	1.6150192E-5	18.5	35
CCACGTT	50	0.0070318743	18.499998	37
ATCGAGC	100	2.870438E-7	18.499998	26
CCGCAAG	185	0.0	18.0	9
CATGCTA	350	0.0	17.97143	4
AGCTTCG	105	4.7898357E-7	17.619047	21
CGCTCTC	105	4.7898357E-7	17.619047	29
>>END_MODULE