FastQCFastQC Report
Fri 10 Feb 2017
ERR1633189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380139
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77132.0289946572174915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61261.611515787646098No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA33500.8812565929830931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32470.8541612410197322No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA26360.693430560926398No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG22090.581103228029747No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20100.5287539557898558No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18850.4958712470964568No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15400.40511497110267564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.3890682092602969No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14230.3743367557656541No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14040.3693385840442575No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC13160.34618915712410464No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12750.33540362867266976No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12400.326196470238518No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12230.32172442185621575No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12200.3209352368475742No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC12170.3201460518389326No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12100.31830462015210226No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11490.30225785830972357No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11060.2909462065191943No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC10730.28226517142413693No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10310.2712165813031549No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10040.2641139162253807No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9840.25885268283443685No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9590.2522761410957571No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA9590.2522761410957571No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9300.24464735267888854No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9070.2385969342793031No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9020.23728162593156713No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8980.23622937925337836No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8880.23359876255790646No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8860.23307263921881205No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8800.23149426920152893No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8680.22833752916696262No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8460.22255017243692438No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA8430.22176098742828282No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC8290.2180781240546221No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8120.21360607567231987No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8110.21334301400277267No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7980.20992321229865918No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC7940.20887096562047042No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7860.20676647226409287No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.20308360889043217No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7700.20255748555133782No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA7540.19834849883858274No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7530.19808543716903554No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7310.19229808043899732No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7250.19071971042171418No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG7230.19019358708261977No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7070.18598460036986472No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7070.18598460036986472No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7030.18493235369167596No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6960.18309092200484559No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6890.18124949031801527No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6770.17809275028344895No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC6730.17704050360526016No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6610.17388376357069388No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6590.1733576402315995No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6450.1696747768579388No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6280.16520272847563652No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6280.16520272847563652No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6160.16204598844107024No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5970.1570478167196736No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC5950.15652169338057922No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5940.15625863171103202No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5930.15599557004148484No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5900.15520638503284326No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC5880.15468026169374885No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT5620.14784065828552187No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5540.14573616492914435No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG5510.14494697992050276No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5510.14494697992050276No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC5460.1436316715727668No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5430.1428424865641252No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5420.14257942489457803No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5420.14257942489457803No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5340.1404749315382005No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG5260.13837043818182299No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG5160.13573982148635105No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5100.13416145146906788No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5100.13416145146906788No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5080.1336353281299735No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5050.13284614312133194No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4980.13100471143450157No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4910.12916327974767125No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4620.12153449133080269No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG4600.12100836799170829No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA4550.11969305964397232No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4530.11916693630487794No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4500.11837775129623637No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC4500.11837775129623637No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA4460.11732550461804761No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.1170624429485004No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC4450.1170624429485004No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4400.11574713460076447No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG4350.11443182625302849No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4340.11416876458348131No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA4290.11285345623574534No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4290.11285345623574534No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4180.10995977787072624No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC4180.10995977787072624No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4090.10759222284480151No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA4080.10732916117525432No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA4050.10653997616661273No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA4040.10627691449706556No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC4020.10575079115797117No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3990.10496160614932959No Hit
TATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACGG3960.104172421140688No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT3820.10048955776702731No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT3810.10022649609748013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAGA200.001840386437.02
ACAACGC352.384135E-531.7142853
AACTGCC551.8488208E-830.27272819
TTGCACT250.00549177429.5999984
GGTATCA13050.028.919541
CTCCAGA604.3068212E-827.7500021
GACCGTT950.027.2631597
ATCTCTA556.2432264E-726.909097
GGCAGGT358.8584324E-426.4285733
TAACAAC358.8584324E-426.428571
AAACCCG358.8584324E-426.4285712
TGGGCAG358.8584324E-426.4285731
GACGAGG358.8584324E-426.4285721
GAGGCAC358.8584324E-426.4285724
ACTGCCT659.357245E-825.61538520
TGAGTAA659.357245E-825.6153853
GTAATGT659.357245E-825.6153856
TCCAGAT659.357245E-825.6153852
TATCTTC659.357245E-825.61538537
GTGAGTA659.357245E-825.6153852
Produced by FastQC (version 0.11.5)