FastQCFastQC Report
Fri 10 Feb 2017
ERR1633188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620756
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50690.8165849383654769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.5820322316659041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21150.3407135815038437No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9660.15561669963721655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.15352247904168465No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8990.14482340887562908No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7600.12243135789263414No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7470.12033713729710226No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7000.11276572437479461No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6520.10503321756052297No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6360.10245571528909911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTC1250.028.11999927
GGTATCA8300.028.0843371
CCGCTCT1350.027.40740628
CGCTCTC1400.026.42857229
CGGGTAT509.082298E-625.89999817
TCGGTAG509.082298E-625.89999828
AGCTTCG1600.025.437521
TTCGCCG1350.024.66666624
GCCGGCA1600.024.2812515
GTATCAA25050.024.0758481
CTCGGTA551.9008316E-523.54545427
CCGGCAG1650.023.54545216
TGCTCGC1650.023.54545210
GCTTCGC1750.023.25714322
GTAGTGT502.7001632E-422.1999996
CGGTAGG603.722732E-521.58333429
GCTCGGT603.722732E-521.58333426
GTTATGC450.003823855420.5555551
TTGTCGC555.139432E-420.18181837
AGGCTCG656.895596E-519.92307724
Produced by FastQC (version 0.11.5)