FastQCFastQC Report
Fri 10 Feb 2017
ERR1633187.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633187.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715781
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56590.7906049476026885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38800.5420652406252751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22530.31476107915689294No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG13530.18902429653762814No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA12320.1721196846521492No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG11280.15759010088281192No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11020.1539577049404776No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG10420.14557525276585995No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10160.14194285682352564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.13803104580870407No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT8500.11875140580708346No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8350.11665579276342902No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC7830.10939100087876041No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7760.108413048125055No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7590.10603802000891335No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT7400.10338357682028441No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC7400.10338357682028441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCG250.00549497629.59999823
ACCGTTC250.00549497629.59999826
GCCGGCA1350.027.40740815
GGTATCA10050.026.5074621
CCGGCAG1450.025.5172416
TGCGGGT1103.6379788E-1223.54545421
CCGCTCT1300.022.7692328
GTATCAA26900.022.763941
CGCTCTC1400.022.46428529
GCCGCTC1400.021.14285727
TTCTGCG1151.3460522E-1020.91304418
AGCTTCG1600.020.812521
GTACTAA450.00382441920.5555551
GCGTTAG555.1404873E-420.18181823
CGTGTCT555.1404873E-420.18181810
CGCGTTA555.1404873E-420.18181822
ACGCGTT555.1404873E-420.18181821
TGCTCGC1900.019.47368410
AACGTCA1254.110916E-1019.2428
GGTAACG1254.110916E-1019.2425
Produced by FastQC (version 0.11.5)