FastQCFastQC Report
Fri 10 Feb 2017
ERR1633186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741772
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24120.3251673020820414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.22918093430326297No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15880.21408195510210687No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC15540.20949833641604157No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG15270.20585840393004862No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13760.18550174447134699No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC13060.17606488247062438No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11750.15840446929784355No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10730.14465361323964776No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10620.14317067778239137No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10450.1408788684393587No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10420.1404744314964706No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10380.13993518223928647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9480.12780207395264315No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9440.127262824695459No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA9370.12631913849538673No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9110.12281401832368977No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8810.11876964889480865No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG8730.11769115038044034No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG8500.11459046715163149No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8460.11405121789444736No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG8390.11310753169437508No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8380.11297271938007906No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG7950.10717578986534945No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT7930.10690616523675739No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7510.10124404803632382No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7480.10083961109343571No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7460.10056998646484365No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT7430.10016554952195554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT250.005495103529.5999986
GGTATCA4050.028.7777791
TGTGCGG358.8664185E-426.4285710
GTATCAA13050.022.3984681
GCCGGCA855.174479E-821.76470615
CGGTCGT603.7239926E-521.58333410
TTCGCCG1001.2878445E-820.34999824
TTAACGG4500.020.14444535
CCGGTCG656.897911E-519.9230779
GTGACAC4550.019.92307724
AACGGCC4600.019.70652237
TAACGGC4700.019.68085136
CATGCTA3400.019.5882364
CACATGT4650.019.49462328
TTTAACG4700.019.28723534
GCACCGC4800.019.27083410
ATTAGAG4750.019.084213
AGGCACC4900.018.5000028
CGGCAAG801.6154108E-518.537
CGATTCC609.2326046E-418.537
Produced by FastQC (version 0.11.5)