##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633186.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741772
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.661660186688092	33.0	33.0	33.0	27.0	33.0
2	31.824777694493726	33.0	33.0	33.0	27.0	33.0
3	31.55840339079933	33.0	33.0	33.0	27.0	33.0
4	31.85802241119913	33.0	33.0	33.0	27.0	33.0
5	31.96693323554947	33.0	33.0	33.0	33.0	33.0
6	35.07240095339269	37.0	37.0	37.0	33.0	37.0
7	35.213353968604906	37.0	37.0	37.0	33.0	37.0
8	35.339454711151134	37.0	37.0	37.0	33.0	37.0
9	35.4627823643923	37.0	37.0	37.0	33.0	37.0
10	35.47091154694434	37.0	37.0	37.0	33.0	37.0
11	35.47456091629234	37.0	37.0	37.0	33.0	37.0
12	35.46494745015989	37.0	37.0	37.0	33.0	37.0
13	35.46594640940882	37.0	37.0	37.0	33.0	37.0
14	35.4432763706368	37.0	37.0	37.0	33.0	37.0
15	35.44361609766882	37.0	37.0	37.0	33.0	37.0
16	35.41930539303182	37.0	37.0	37.0	33.0	37.0
17	35.43006746008207	37.0	37.0	37.0	33.0	37.0
18	35.415185528706935	37.0	37.0	37.0	33.0	37.0
19	35.406766499679144	37.0	37.0	37.0	33.0	37.0
20	35.4042859530961	37.0	37.0	37.0	33.0	37.0
21	35.41677631401563	37.0	37.0	37.0	33.0	37.0
22	35.31530982566071	37.0	37.0	37.0	33.0	37.0
23	35.37519480379416	37.0	37.0	37.0	33.0	37.0
24	35.34533657242387	37.0	37.0	37.0	33.0	37.0
25	35.367466013815566	37.0	37.0	37.0	33.0	37.0
26	35.25366015433314	37.0	37.0	37.0	33.0	37.0
27	35.2804203987209	37.0	37.0	37.0	33.0	37.0
28	35.300093559746124	37.0	37.0	37.0	33.0	37.0
29	35.29991425936811	37.0	37.0	37.0	33.0	37.0
30	35.292008865257785	37.0	37.0	37.0	33.0	37.0
31	35.266664150170136	37.0	37.0	37.0	33.0	37.0
32	35.276318059996875	37.0	37.0	37.0	33.0	37.0
33	35.27077997012559	37.0	37.0	37.0	33.0	37.0
34	35.25640223680592	37.0	37.0	37.0	33.0	37.0
35	35.187072307932894	37.0	37.0	37.0	33.0	37.0
36	35.23011653176448	37.0	37.0	37.0	33.0	37.0
37	35.229172845564406	37.0	37.0	37.0	33.0	37.0
38	35.214982501361604	37.0	37.0	37.0	33.0	37.0
39	35.12588773908964	37.0	37.0	37.0	33.0	37.0
40	34.924913585306534	37.0	37.0	37.0	27.0	37.0
41	35.10410476534569	37.0	37.0	37.0	27.0	37.0
42	35.12916098208075	37.0	37.0	37.0	33.0	37.0
43	34.77941739510254	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	30.0
16	37.0
17	28.0
18	29.0
19	36.0
20	57.0
21	192.0
22	433.0
23	1145.0
24	2121.0
25	3647.0
26	5717.0
27	8482.0
28	11812.0
29	15597.0
30	20309.0
31	25733.0
32	32365.0
33	42587.0
34	61367.0
35	121941.0
36	388103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53577109947531	21.362898572607218	13.6481290747022	24.453201253215273
2	16.415421450256954	21.4076562609535	38.71068198853556	23.466240300253986
3	17.121837977168187	28.26946824630749	29.531446320432693	25.077247456091627
4	12.165463242074384	18.231073699195978	40.41511407818036	29.18834898054928
5	13.090140905830902	37.7057909977729	36.51256720393868	12.691500892457523
6	28.691026353111198	41.251220051444385	17.20569123666032	12.8520623587841
7	26.48010439865619	32.01698095910873	24.490274639646685	17.012640002588398
8	23.898044142944194	36.48573955339376	20.517220925028177	19.098995378633866
9	25.708438711625675	15.386533867549598	20.580447900433015	38.32457952039171
10	15.204402430935652	27.865435740362265	34.528803999072494	22.40135782962959
11	33.57042325674197	24.16241109127872	24.111182411846226	18.155983240133086
12	22.669229898135814	26.821179553825164	29.521065772231896	20.98852477580712
13	26.91770517086113	22.666938088792783	26.245530971781083	24.169825768565005
14	21.693323554946804	21.15393948544836	27.575993701568674	29.576743258036164
15	23.87633936034253	29.0209120861936	24.84051703218779	22.262231521276078
16	22.999115631218217	28.436365891405984	25.40335844437374	23.161160033002055
17	22.136856068980766	27.950097873740177	26.22166919215069	23.69137686512837
18	22.378978985456445	28.019121778659752	27.838473277503063	21.76342595838074
19	24.564691037138093	26.330327917473294	27.63800736614485	21.466973679243758
20	23.62841951435212	26.716969634874328	27.963039855912598	21.691570994860953
21	22.36778956336988	27.62681794405828	27.783200228641686	22.222192263930157
22	22.3057758987937	27.053865608300125	27.661464708832362	22.97889378407381
23	22.359296387569227	27.117766645276447	28.49918842986794	22.023748537286387
24	22.45002507509046	28.252077457763303	25.995723753390532	23.302173713755707
25	22.83437498314846	27.469896410217693	27.882556904277866	21.81317170235598
26	22.698214545709465	27.781986917813022	27.786570536499084	21.73322799997843
27	22.36131857228367	27.772684868126596	26.48738426362818	23.378612295961563
28	22.077673463004803	27.100375856732256	28.69668847031163	22.125262209951305
29	24.318928188176418	26.80163176825224	27.008568670696658	21.870871372874685
30	23.159137848287614	26.736247795818663	27.66523945363265	22.439374902261072
31	22.804716274003333	27.056966291528934	27.858560312333168	22.279757122134562
32	21.771110260295618	26.870790485486108	27.91423779813743	23.443861456080846
33	21.613919101826436	27.044967995556586	28.56241540527278	22.778697497344197
34	23.087687321710714	26.380612910705715	28.29508258602374	22.236617181559833
35	22.742837421741452	27.516811095592715	27.39345782801184	22.34689365465399
36	23.686793246442303	27.343172834779423	26.707937209816496	22.262096708961785
37	22.526059220353424	26.289479786241593	27.602686539799294	23.58177445360569
38	22.826151431976403	27.455471492588018	27.12774275653435	22.590634318901227
39	22.11488166175051	27.31054825471978	27.886870898335335	22.687699185194372
40	22.43411722200353	27.84777532718949	28.026806080574623	21.691301370232363
41	22.368463624941356	26.6259713227245	27.636659243001894	23.368905809332247
42	21.850379901101686	27.95791698796935	27.49146638050506	22.700236730423903
43	23.409619128249652	26.304848390071346	27.329556791035518	22.955975690643484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	31.5
2	43.0
3	130.0
4	217.0
5	217.0
6	282.0
7	347.0
8	318.5
9	290.0
10	403.5
11	517.0
12	517.0
13	857.5
14	1198.0
15	2135.0
16	3072.0
17	3581.5
18	4091.0
19	4091.0
20	3825.5
21	3560.0
22	3865.0
23	4170.0
24	5673.0
25	7176.0
26	7176.0
27	9839.5
28	12503.0
29	15862.5
30	19222.0
31	23131.0
32	27040.0
33	27040.0
34	34626.0
35	42212.0
36	47023.5
37	51835.0
38	56742.0
39	61649.0
40	61649.0
41	65535.0
42	69421.0
43	70204.5
44	70988.0
45	68211.5
46	65435.0
47	65435.0
48	61081.0
49	56727.0
50	55872.5
51	55018.0
52	50257.0
53	45496.0
54	45496.0
55	44413.5
56	43331.0
57	35214.0
58	27097.0
59	25083.5
60	23070.0
61	23070.0
62	20365.0
63	17660.0
64	14224.0
65	10788.0
66	8722.5
67	6657.0
68	6657.0
69	5479.5
70	4302.0
71	3509.0
72	2716.0
73	2183.0
74	1650.0
75	1650.0
76	1320.5
77	991.0
78	757.5
79	524.0
80	429.0
81	334.0
82	334.0
83	254.5
84	175.0
85	153.0
86	131.0
87	102.0
88	73.0
89	73.0
90	48.0
91	23.0
92	13.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	741772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.93273666243945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.9326486377039	39.011889839191504
2	8.169525601949141	7.504973362627741
3	2.220704424436381	3.060090945982514
4	1.0519192183744643	1.932701137910136
5	0.5975357077655038	1.3723225155598628
6	0.4280704873771047	1.1797469381792798
7	0.2753859328540127	0.8854460674026519
8	0.22540001908059318	0.8282591776110172
9	0.17807398419077844	0.7361482880039788
>10	1.5033377654643194	14.302285580162854
>50	0.21542170843014646	6.977351464246671
>100	0.18614107575268782	15.888806784376039
>500	0.01195127292152067	3.863680219395673
>1k	0.0038841636994942177	2.4562976793501137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2412	0.3251673020820414	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1700	0.22918093430326297	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1588	0.21408195510210687	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1554	0.20949833641604157	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1527	0.20585840393004862	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1376	0.18550174447134699	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1306	0.17606488247062438	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1175	0.15840446929784355	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1073	0.14465361323964776	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1062	0.14317067778239137	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1045	0.1408788684393587	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1042	0.1404744314964706	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1038	0.13993518223928647	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	948	0.12780207395264315	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	944	0.127262824695459	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	937	0.12631913849538673	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	911	0.12281401832368977	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	881	0.11876964889480865	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	873	0.11769115038044034	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	850	0.11459046715163149	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	846	0.11405121789444736	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	839	0.11310753169437508	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	838	0.11297271938007906	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	795	0.10717578986534945	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	793	0.10690616523675739	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	751	0.10124404803632382	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	748	0.10083961109343571	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	746	0.10056998646484365	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	743	0.10016554952195554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	1.3481231429603705E-4	1.3481231429603705E-4	0.0
5	0.0	0.0	1.3481231429603705E-4	1.3481231429603705E-4	0.0
6	0.0	0.0	6.740615714801853E-4	1.3481231429603705E-4	0.0
7	0.0	0.0	0.0016177477715524447	1.3481231429603705E-4	0.0
8	0.0	0.0	0.0017525600858484818	1.3481231429603705E-4	0.0
9	0.0	0.0	0.002022184714440556	0.0010784985143682964	0.0
10	1.3481231429603705E-4	0.0	0.0026962462859207413	0.0010784985143682964	0.0
11	1.3481231429603705E-4	0.0	0.0045836186860652595	0.0010784985143682964	0.0
12	1.3481231429603705E-4	0.0	0.005662117200433556	0.0010784985143682964	0.0
13	1.3481231429603705E-4	0.0	0.006336178771913742	0.0012133108286643335	0.0
14	1.3481231429603705E-4	0.0	0.0066058034005058155	0.0013481231429603707	0.0
15	1.3481231429603705E-4	0.0	0.007279864971986001	0.0013481231429603707	0.0
16	1.3481231429603705E-4	0.0	0.008088738857762224	0.0013481231429603707	0.0
17	1.3481231429603705E-4	0.0	0.008088738857762224	0.0013481231429603707	0.0
18	1.3481231429603705E-4	0.0	0.00822355117205826	0.0014829354572564075	0.0
19	1.3481231429603705E-4	0.0	0.008358363486354298	0.0017525600858484818	0.0
20	1.3481231429603705E-4	0.0	0.008627988114946371	0.002022184714440556	0.0
21	1.3481231429603705E-4	0.0	0.008762800429242408	0.0022918093430326298	0.0
22	1.3481231429603705E-4	0.0	0.008897612743538445	0.002561433971624704	0.0
23	1.3481231429603705E-4	0.0	0.009032425057834482	0.002965870914512815	0.0
24	1.3481231429603705E-4	0.0	0.009032425057834482	0.0032354955431048893	0.0
25	1.3481231429603705E-4	0.0	0.009032425057834482	0.0036399324859930004	0.0
26	1.3481231429603705E-4	0.0	0.009032425057834482	0.0037747448002890377	0.0
27	1.3481231429603705E-4	0.0	0.009032425057834482	0.005122867943249408	0.0
28	1.3481231429603705E-4	0.0	0.009032425057834482	0.010245735886498816	0.0
29	1.3481231429603705E-4	0.0	0.009032425057834482	0.019278160944333298	0.0
30	1.3481231429603705E-4	0.0	0.009032425057834482	0.034511952459785486	0.0
31	1.3481231429603705E-4	0.0	0.009032425057834482	0.08277476097776676	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	25	0.0054951035	29.599998	6
GGTATCA	405	0.0	28.777779	1
TGTGCGG	35	8.8664185E-4	26.42857	10
GTATCAA	1305	0.0	22.398468	1
GCCGGCA	85	5.174479E-8	21.764706	15
CGGTCGT	60	3.7239926E-5	21.583334	10
TTCGCCG	100	1.2878445E-8	20.349998	24
TTAACGG	450	0.0	20.144445	35
CCGGTCG	65	6.897911E-5	19.923077	9
GTGACAC	455	0.0	19.923077	24
AACGGCC	460	0.0	19.706522	37
TAACGGC	470	0.0	19.680851	36
CATGCTA	340	0.0	19.588236	4
CACATGT	465	0.0	19.494623	28
TTTAACG	470	0.0	19.287235	34
GCACCGC	480	0.0	19.270834	10
ATTAGAG	475	0.0	19.08421	3
AGGCACC	490	0.0	18.500002	8
CGGCAAG	80	1.6154108E-5	18.5	37
CGATTCC	60	9.2326046E-4	18.5	37
>>END_MODULE