FastQCFastQC Report
Fri 10 Feb 2017
ERR1633185.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633185.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637259
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36600.5743347681241066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.3932467018904402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13750.2157678432160236No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11540.18108806623366638No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10560.16570970358990614No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10490.16461124911535183No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8920.1399744844717768No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8780.13777757552266817No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8370.13134377074313583No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7820.12271305701449489No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7650.12004538186200586No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7390.11596540809937561No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7250.113768499150267No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7120.11172851226895186No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC7090.11125774606557146No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7030.11031621365881063No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6990.10968852538763674No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6940.10890391504866938No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6500.10199934406575663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.10137165579458274No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6420.10074396752340885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.028.4615381
GTATCAA19200.023.3177071
CCTTATA400.001930248623.1252
GTCAACC2700.021.24074215
ATAATCG450.003823965820.55555515
TGCGGTA450.003823965820.55555536
TTCGCCG1351.8189894E-1220.55555524
CGTCCCA450.003823965820.55555531
CATAATA1450.020.4137942
CAACCCA2900.019.77586217
TTTCGGG759.255151E-619.73333422
TATTAGA3300.019.6212142
CATGCTA2950.019.440684
TACATGC3050.019.4098362
ATAGTAC2600.019.2115383
TTAACGG3100.019.09677335
CATGCTC3050.018.8032831
TCAACCC3050.018.8032816
GTTCTAG609.2306605E-418.51
GTACATG3100.018.51
Produced by FastQC (version 0.11.5)