##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637259
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.825758443584164	33.0	33.0	33.0	27.0	33.0
2	31.940449644493054	33.0	33.0	33.0	33.0	33.0
3	31.66989246130694	33.0	33.0	33.0	27.0	33.0
4	31.947065478871227	33.0	33.0	33.0	33.0	33.0
5	32.0425776646544	33.0	33.0	33.0	33.0	33.0
6	35.1886595560047	37.0	37.0	37.0	33.0	37.0
7	35.337641367167826	37.0	37.0	37.0	33.0	37.0
8	35.46516094711883	37.0	37.0	37.0	33.0	37.0
9	35.58148099909142	37.0	37.0	37.0	33.0	37.0
10	35.59234628306544	37.0	37.0	37.0	33.0	37.0
11	35.61669117266292	37.0	37.0	37.0	33.0	37.0
12	35.59470952940641	37.0	37.0	37.0	33.0	37.0
13	35.60050152292867	37.0	37.0	37.0	33.0	37.0
14	35.58490033094864	37.0	37.0	37.0	33.0	37.0
15	35.58991399101464	37.0	37.0	37.0	33.0	37.0
16	35.57331006702142	37.0	37.0	37.0	33.0	37.0
17	35.56780994854525	37.0	37.0	37.0	33.0	37.0
18	35.54642460914636	37.0	37.0	37.0	33.0	37.0
19	35.53939136206785	37.0	37.0	37.0	33.0	37.0
20	35.54438305304436	37.0	37.0	37.0	33.0	37.0
21	35.54727983441583	37.0	37.0	37.0	33.0	37.0
22	35.44682146505581	37.0	37.0	37.0	33.0	37.0
23	35.49484903312468	37.0	37.0	37.0	33.0	37.0
24	35.48672831611637	37.0	37.0	37.0	33.0	37.0
25	35.5001671220022	37.0	37.0	37.0	33.0	37.0
26	35.388030612356985	37.0	37.0	37.0	33.0	37.0
27	35.39760128927171	37.0	37.0	37.0	33.0	37.0
28	35.41700627217505	37.0	37.0	37.0	33.0	37.0
29	35.417585314605205	37.0	37.0	37.0	33.0	37.0
30	35.4239579197783	37.0	37.0	37.0	33.0	37.0
31	35.40716098164169	37.0	37.0	37.0	33.0	37.0
32	35.40366005030921	37.0	37.0	37.0	33.0	37.0
33	35.4004039174025	37.0	37.0	37.0	33.0	37.0
34	35.38428959026079	37.0	37.0	37.0	33.0	37.0
35	35.31564246248386	37.0	37.0	37.0	33.0	37.0
36	35.340244704272514	37.0	37.0	37.0	33.0	37.0
37	35.342174845706374	37.0	37.0	37.0	33.0	37.0
38	35.327345710299895	37.0	37.0	37.0	33.0	37.0
39	35.210887566907644	37.0	37.0	37.0	33.0	37.0
40	35.00516273603041	37.0	37.0	37.0	27.0	37.0
41	35.17949844568692	37.0	37.0	37.0	33.0	37.0
42	35.20848352082905	37.0	37.0	37.0	33.0	37.0
43	34.87904447014479	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	31.0
16	37.0
17	25.0
18	28.0
19	22.0
20	55.0
21	113.0
22	306.0
23	715.0
24	1550.0
25	2695.0
26	4388.0
27	6573.0
28	9261.0
29	12395.0
30	16248.0
31	20650.0
32	26033.0
33	34941.0
34	49857.0
35	99480.0
36	351855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.74017157858893	20.354204491423424	13.639666132608564	24.265957797379087
2	16.340137997266417	21.47541266580778	38.990740028779506	23.193709308146296
3	17.741295140594325	27.75715996164825	29.83731889231851	24.66422600543892
4	12.23207518450112	17.91234019448921	39.93886316238766	29.916721458622003
5	13.800354330029077	37.01006968909031	35.19102907922838	13.998546901652231
6	29.128188067959808	40.29790085349912	17.082693222065124	13.491217856475938
7	26.69856369231349	32.224260465525006	23.331016117465584	17.74615972469592
8	24.69906270448907	35.395969299766655	20.296771014611014	19.60819698113326
9	26.442937643877922	15.040195587665298	20.008505176074408	38.50836159238237
10	15.625828744670534	27.52318915856818	33.328207212452085	23.522774884309207
11	34.02572580379406	23.893110964301798	23.603746671290637	18.477416560613502
12	23.629795734544352	26.008734282293382	29.343955911175833	21.01751407198643
13	27.931971145170177	22.41192356639922	25.946906987582757	23.70919830084785
14	22.4123943326026	20.849136693244034	27.86763309737485	28.870835876778518
15	24.46885803103605	27.97465394761	25.218160904749876	22.33832711660408
16	22.88943741869475	27.980460064118358	25.18583495878442	23.944267558402473
17	22.45115408334759	27.160856104033055	25.872682849516444	24.515306963102915
18	23.21928760519663	26.58620749177336	27.885836057238894	22.308668845791114
19	25.579238582742654	25.42639648871181	27.42872207375651	21.565642854789026
20	24.630644682931116	25.597127698471105	27.722637106733682	22.049590511864093
21	23.46424295302224	26.674868460076674	27.601179426261535	22.259709160639552
22	23.03443340933592	26.525478651537288	27.447238877756142	22.992849061370652
23	22.95973850506623	26.563296869875515	28.181320310893998	22.295644314164257
24	23.342942194617887	27.033435384984756	26.235015904051572	23.388606516345785
25	23.165620258011263	26.675496148347843	27.88834681032359	22.2705367833173
26	23.40602486587086	26.816255243158587	27.581407245719557	22.19631264525099
27	23.433329305666927	26.696052939228792	26.766354025600265	23.104263729504016
28	22.570101010735037	26.312378483473754	28.131419093335673	22.986101412455533
29	24.42931366995209	26.144785715070324	26.902876224580584	22.523024390396998
30	23.63952490274755	25.94847620826069	27.861826980866493	22.550171908125268
31	23.228702929264237	26.076524615580166	27.550022832160863	23.14474962299473
32	22.036565980237235	26.033527969004755	28.145385157369297	23.784520893388716
33	22.362963881247655	26.22403135930603	28.385789765228896	23.02721499421742
34	23.057344031233768	26.075112316970024	28.129692950589945	22.73785070120626
35	23.118700559741015	26.884987108852133	27.190828218981604	22.805484112425244
36	23.877104913386866	26.632970267975814	26.790676946108256	22.699247872529067
37	23.0973591585211	26.031331060055646	27.36862092179161	23.50268885963164
38	23.54380244139353	26.39695947801443	26.93363294986811	23.12560513072393
39	22.630359084767733	26.460355993402995	27.662535954768785	23.246748967060487
40	22.973076880828675	27.08929964111923	27.678698927751512	22.258924550300584
41	22.93227714320237	25.76989889511172	27.691409615242783	23.606414346443124
42	22.260179926842934	27.255166266776932	27.480820200263945	23.003833606116196
43	23.403043346582784	25.740240624298753	27.336294975826153	23.520421053292303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	36.0
2	45.0
3	172.0
4	299.0
5	299.0
6	397.5
7	496.0
8	471.0
9	446.0
10	607.0
11	768.0
12	768.0
13	1243.5
14	1719.0
15	3097.0
16	4475.0
17	5050.0
18	5625.0
19	5625.0
20	4985.0
21	4345.0
22	4410.5
23	4476.0
24	5728.0
25	6980.0
26	6980.0
27	8869.5
28	10759.0
29	13187.0
30	15615.0
31	18680.0
32	21745.0
33	21745.0
34	26916.5
35	32088.0
36	35503.0
37	38918.0
38	42063.0
39	45208.0
40	45208.0
41	47745.5
42	50283.0
43	51790.5
44	53298.0
45	51831.0
46	50364.0
47	50364.0
48	48977.0
49	47590.0
50	47650.0
51	47710.0
52	45568.0
53	43426.0
54	43426.0
55	42524.5
56	41623.0
57	35672.5
58	29722.0
59	27046.5
60	24371.0
61	24371.0
62	21784.5
63	19198.0
64	15231.0
65	11264.0
66	9575.0
67	7886.0
68	7886.0
69	6598.5
70	5311.0
71	4481.5
72	3652.0
73	3475.5
74	3299.0
75	3299.0
76	2717.5
77	2136.0
78	1643.5
79	1151.0
80	787.0
81	423.0
82	423.0
83	317.5
84	212.0
85	192.5
86	173.0
87	128.0
88	83.0
89	83.0
90	60.0
91	37.0
92	23.5
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	637259.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.10632703176411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.39568959321588	44.153663664856694
2	7.410691914667689	7.574664890453139
3	1.9625576502617856	3.00897339278908
4	0.9129488891839149	1.8662985797567568
5	0.5804421976875743	1.4832134389028522
6	0.3549666979303328	1.0884626489887808
7	0.2919791260480306	1.0445386491581528
8	0.21566284900842148	0.8817388872021081
9	0.17052065182678403	0.7843215778136287
>10	1.37943604340775	14.28300786338032
>50	0.1789479494709769	6.377660296660573
>100	0.13491363442234452	12.632105247956742
>500	0.009369002390440577	3.0976157816231304
>1k	0.0018738004780881152	1.7237350804580487
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3660	0.5743347681241066	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2506	0.3932467018904402	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1375	0.2157678432160236	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1154	0.18108806623366638	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1056	0.16570970358990614	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1049	0.16461124911535183	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	892	0.1399744844717768	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	878	0.13777757552266817	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	837	0.13134377074313583	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	782	0.12271305701449489	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	765	0.12004538186200586	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	739	0.11596540809937561	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	725	0.113768499150267	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	712	0.11172851226895186	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	709	0.11125774606557146	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	703	0.11031621365881063	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	699	0.10968852538763674	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	694	0.10890391504866938	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	650	0.10199934406575663	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	646	0.10137165579458274	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	642	0.10074396752340885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.5692206779347172E-4	0.0	0.0
3	0.0	0.0	3.1384413558694345E-4	0.0	0.0
4	0.0	0.0	4.7076620338041517E-4	0.0	0.0
5	1.5692206779347172E-4	0.0	7.846103389673587E-4	0.0	0.0
6	1.5692206779347172E-4	0.0	0.0020399868813151326	0.0	0.0
7	1.5692206779347172E-4	0.0	0.005178428237184567	0.0	0.0
8	1.5692206779347172E-4	0.0	0.006119960643945398	1.5692206779347172E-4	0.0
9	1.5692206779347172E-4	0.0	0.007218415118499699	6.276882711738869E-4	0.0
10	3.1384413558694345E-4	0.0	0.00815994752526053	6.276882711738869E-4	0.0
11	3.1384413558694345E-4	0.0	0.011612233016716909	7.846103389673587E-4	0.0
12	3.1384413558694345E-4	0.0	0.012082999220097323	7.846103389673587E-4	0.0
13	3.1384413558694345E-4	0.0	0.013024531626858153	9.415324067608303E-4	0.0
14	4.7076620338041517E-4	0.0	0.01365221989803204	9.415324067608303E-4	0.0
15	4.7076620338041517E-4	0.0	0.015064518508173285	9.415324067608303E-4	0.0
16	6.276882711738869E-4	0.0	0.016162972982727588	0.0010984544745543021	0.0
17	6.276882711738869E-4	0.0	0.01631989505052106	0.0014122986101412455	0.0
18	6.276882711738869E-4	0.0	0.016947583321694947	0.0014122986101412455	0.0
19	6.276882711738869E-4	0.0	0.01726142745728189	0.0014122986101412455	0.0
20	6.276882711738869E-4	0.0	0.017732193660662306	0.0015692206779347173	0.0
21	6.276882711738869E-4	0.0	0.018673726067423135	0.0015692206779347173	0.0
22	6.276882711738869E-4	0.0	0.01898757020301008	0.0020399868813151326	0.0
23	6.276882711738869E-4	0.0	0.019144492270803552	0.0021969089491086042	0.0
24	6.276882711738869E-4	0.0	0.01930141433859702	0.0026676751524890192	0.0
25	6.276882711738869E-4	0.0	0.019615258474183966	0.002981519288075963	0.0
26	6.276882711738869E-4	0.0	0.019929102609770908	0.0031384413558694347	0.0
27	6.276882711738869E-4	0.0	0.019929102609770908	0.005021506169391095	0.0
28	7.846103389673587E-4	0.0	0.019929102609770908	0.015535284711553701	0.0
29	7.846103389673587E-4	0.0	0.019929102609770908	0.033110556304422535	0.0
30	7.846103389673587E-4	0.0	0.019929102609770908	0.05947346369372578	0.0
31	7.846103389673587E-4	0.0	0.019929102609770908	0.12177152460773406	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	650	0.0	28.461538	1
GTATCAA	1920	0.0	23.317707	1
CCTTATA	40	0.0019302486	23.125	2
GTCAACC	270	0.0	21.240742	15
ATAATCG	45	0.0038239658	20.555555	15
TGCGGTA	45	0.0038239658	20.555555	36
TTCGCCG	135	1.8189894E-12	20.555555	24
CGTCCCA	45	0.0038239658	20.555555	31
CATAATA	145	0.0	20.413794	2
CAACCCA	290	0.0	19.775862	17
TTTCGGG	75	9.255151E-6	19.733334	22
TATTAGA	330	0.0	19.621214	2
CATGCTA	295	0.0	19.44068	4
TACATGC	305	0.0	19.409836	2
ATAGTAC	260	0.0	19.211538	3
TTAACGG	310	0.0	19.096773	35
CATGCTC	305	0.0	18.80328	31
TCAACCC	305	0.0	18.80328	16
GTTCTAG	60	9.2306605E-4	18.5	1
GTACATG	310	0.0	18.5	1
>>END_MODULE