FastQCFastQC Report
Fri 10 Feb 2017
ERR1633184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776809
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27610.35542842577776523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19090.24574895501983113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11940.15370573718893576No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7980.10272795500567064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.027.672271
TAAACGA358.866797E-426.4285729
TGTCGAC358.866797E-426.4285736
GTATCAA15350.022.2964171
CCGTAAA450.003824707120.55555726
CGGGCTA450.003824707120.55555716
GCGTATA450.003824707120.5555579
GTAAACG450.003824707120.55555728
TTAACGG1650.020.18181835
AGCGTAT500.007032879618.58
GATGTCG500.007032879618.534
GATCGAC500.007032879618.531
ATAAGTC500.007032879618.53
CGCCGTA609.233142E-418.524
GCCGTAA500.007032879618.525
ATCGACA500.007032879618.532
TACCGGC500.007032879618.57
TAACGGC1900.017.52631636
GTCAACC1705.456968E-1217.41176415
GTATTAG3350.016.5671631
Produced by FastQC (version 0.11.5)