##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633184.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	776809
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81942922906403	33.0	33.0	33.0	27.0	33.0
2	31.952555904990803	33.0	33.0	33.0	33.0	33.0
3	31.670959013090734	33.0	33.0	33.0	27.0	33.0
4	31.942974399112266	33.0	33.0	33.0	33.0	33.0
5	32.059770162292146	33.0	33.0	33.0	33.0	33.0
6	35.214338402361456	37.0	37.0	37.0	33.0	37.0
7	35.36153674841563	37.0	37.0	37.0	33.0	37.0
8	35.49667936391056	37.0	37.0	37.0	33.0	37.0
9	35.61106269366086	37.0	37.0	37.0	33.0	37.0
10	35.6267821304851	37.0	37.0	37.0	33.0	37.0
11	35.650397974276814	37.0	37.0	37.0	33.0	37.0
12	35.635084042538125	37.0	37.0	37.0	33.0	37.0
13	35.64064268050448	37.0	37.0	37.0	33.0	37.0
14	35.60913171706301	37.0	37.0	37.0	33.0	37.0
15	35.624020833950176	37.0	37.0	37.0	33.0	37.0
16	35.61544214858479	37.0	37.0	37.0	33.0	37.0
17	35.6040751330121	37.0	37.0	37.0	33.0	37.0
18	35.588395603037554	37.0	37.0	37.0	33.0	37.0
19	35.57970363371176	37.0	37.0	37.0	33.0	37.0
20	35.588086646781896	37.0	37.0	37.0	33.0	37.0
21	35.589170568312156	37.0	37.0	37.0	33.0	37.0
22	35.494631241399105	37.0	37.0	37.0	33.0	37.0
23	35.53977361230367	37.0	37.0	37.0	33.0	37.0
24	35.5179110952628	37.0	37.0	37.0	33.0	37.0
25	35.55682156102723	37.0	37.0	37.0	33.0	37.0
26	35.44562048071019	37.0	37.0	37.0	33.0	37.0
27	35.45940765361885	37.0	37.0	37.0	33.0	37.0
28	35.483082714026224	37.0	37.0	37.0	33.0	37.0
29	35.491492760768736	37.0	37.0	37.0	33.0	37.0
30	35.48624307905804	37.0	37.0	37.0	33.0	37.0
31	35.488121275628885	37.0	37.0	37.0	33.0	37.0
32	35.48137573071373	37.0	37.0	37.0	33.0	37.0
33	35.47960051956144	37.0	37.0	37.0	33.0	37.0
34	35.45610954558971	37.0	37.0	37.0	33.0	37.0
35	35.39908008274878	37.0	37.0	37.0	33.0	37.0
36	35.433962531330096	37.0	37.0	37.0	33.0	37.0
37	35.43324163340023	37.0	37.0	37.0	33.0	37.0
38	35.43220534262605	37.0	37.0	37.0	33.0	37.0
39	35.334268784218516	37.0	37.0	37.0	33.0	37.0
40	35.12690893128169	37.0	37.0	37.0	33.0	37.0
41	35.30065691823859	37.0	37.0	37.0	33.0	37.0
42	35.35582491963919	37.0	37.0	37.0	33.0	37.0
43	35.02487998980445	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	28.0
16	37.0
17	34.0
18	21.0
19	27.0
20	43.0
21	116.0
22	367.0
23	830.0
24	1780.0
25	3066.0
26	5071.0
27	7614.0
28	10622.0
29	14547.0
30	18853.0
31	24227.0
32	31038.0
33	41108.0
34	59317.0
35	119749.0
36	438307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34272388708164	20.46976798672518	13.67324528938259	24.514262836810595
2	16.037275572244912	21.39573563128131	38.855368565503234	23.71162023097055
3	17.910709067479907	27.031355197995904	29.90554949801045	25.152386236513735
4	12.595116688915809	17.817378531917115	39.31403987337943	30.27346490578765
5	13.962634315513853	36.93353192354877	35.37188678298012	13.731946977957257
6	29.84092614786904	39.65698131715776	17.252889706478683	13.249202828494521
7	26.73913407285446	32.05202308418157	23.502559831309885	17.706283011654087
8	24.57064735346784	36.14788191176982	20.394974826501752	18.88649590826059
9	25.807888425597543	15.55865083952426	20.336401869700275	38.297058865177924
10	15.085046645958014	28.149390648151606	33.75810527426948	23.0074574316209
11	34.02252033640187	23.59885119765605	24.14145562165217	18.23717284428991
12	23.028955637743643	25.653667761315845	30.28672427842623	21.030652322514285
13	28.05992206578451	22.186148718668296	26.145423134901886	23.608506080645306
14	22.09770999048672	20.999756696948673	27.57885142937324	29.323681883191366
15	23.831083316490925	28.760737839031215	24.231310399338835	23.176868445139025
16	23.00681377275495	28.34609279758602	25.099606209505808	23.547487220153215
17	22.445285778099894	27.71466345008876	26.399282191632693	23.440768580178652
18	23.29066733263904	27.280451179118675	27.364770490558165	22.064110997684118
19	24.742633002449764	26.495573557978858	27.148887306918436	21.61290613265294
20	24.24662948034845	26.330796888295577	27.38575376958815	22.036819861767825
21	23.308174853792888	26.90249469303265	27.327438276333048	22.46189217684141
22	22.86340657742122	26.820621285283767	27.346748042311557	22.96922409498345
23	22.874477509915565	26.751106127761137	27.732557166562177	22.64185919576112
24	23.012349239002123	27.216214024296836	26.554403978326718	23.217032758374323
25	23.218577539652603	26.82229479833524	27.344945797486897	22.614181864525257
26	23.20918012020973	26.950640376205737	27.343401016208617	22.496778487375916
27	23.083666641349417	27.028008171892964	26.868509504910477	23.01981568184715
28	22.70223439738726	26.96338482175155	27.51165344376803	22.82272733709316
29	23.732989705320097	26.806332058459674	26.891809955857877	22.568868280362352
30	23.381938159830796	26.436356942311427	27.397983288041207	22.78372160981657
31	23.35657800051235	26.612462008035436	27.102543868570013	22.9284161228822
32	22.433442454966407	26.621988159251504	27.574989476177542	23.36957990960455
33	22.588692973433623	26.68107604314574	27.781475240374405	22.94875574304623
34	23.117780561244786	26.45283460927976	27.549629316859097	22.879755512616356
35	23.045433304711967	27.040237690346018	27.244535014398647	22.66979399054336
36	23.750883421793517	26.664083449084654	26.84392173623117	22.74111139289066
37	23.179700544149206	26.377397790190383	27.05941872455134	23.383482941109076
38	23.06564419310281	26.87533228888955	27.114644655249876	22.94437886275777
39	22.866496139977784	26.669618915331828	27.28251088748972	23.181374057200678
40	22.975145756550194	27.178238151205765	27.442267018018583	22.40434907422545
41	22.96407482405585	26.227039079104387	27.459645807399248	23.349240289440516
42	22.652672664709087	27.118506608445575	27.291779575159403	22.937041151685936
43	23.236728719672403	26.2307723005269	27.231018178213688	23.301480801587005
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	26.5
2	35.0
3	147.5
4	260.0
5	260.0
6	330.0
7	400.0
8	373.5
9	347.0
10	481.5
11	616.0
12	616.0
13	982.5
14	1349.0
15	2532.5
16	3716.0
17	4400.5
18	5085.0
19	5085.0
20	5115.5
21	5146.0
22	5939.0
23	6732.0
24	8606.5
25	10481.0
26	10481.0
27	13631.0
28	16781.0
29	20109.5
30	23438.0
31	26944.0
32	30450.0
33	30450.0
34	35997.0
35	41544.0
36	44829.5
37	48115.0
38	51304.5
39	54494.0
40	54494.0
41	56801.5
42	59109.0
43	60209.5
44	61310.0
45	60495.0
46	59680.0
47	59680.0
48	58159.5
49	56639.0
50	56264.5
51	55890.0
52	52928.0
53	49966.0
54	49966.0
55	48578.5
56	47191.0
57	41862.5
58	36534.0
59	33710.5
60	30887.0
61	30887.0
62	27386.5
63	23886.0
64	20070.0
65	16254.0
66	13778.0
67	11302.0
68	11302.0
69	9408.5
70	7515.0
71	6199.5
72	4884.0
73	3846.5
74	2809.0
75	2809.0
76	2222.5
77	1636.0
78	1253.5
79	871.0
80	723.0
81	575.0
82	575.0
83	485.0
84	395.0
85	315.5
86	236.0
87	191.5
88	147.0
89	147.0
90	109.0
91	71.0
92	42.0
93	13.0
94	7.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	776809.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.403991349516446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.22864878020617	54.038365099911665
2	8.690181072557145	11.019843311002896
3	2.3933368712317074	4.552413308401617
4	1.0717103265977779	2.718028491071718
5	0.6134484328109878	1.9447539563661143
6	0.39668675618036076	1.5090914194396399
7	0.2556145062645008	1.1344885960803728
8	0.20281126085198958	1.0287234742915257
9	0.1479030523918935	0.8439879468980424
>10	0.8652824396679841	10.348341299430011
>50	0.08181279603602404	3.5798423292791237
>100	0.05051842484701667	5.939165685609515
>500	0.0014316962495181267	0.5825177896216633
>1k	6.1358410693634E-4	0.7604372925960449
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2761	0.35542842577776523	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1909	0.24574895501983113	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1194	0.15370573718893576	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	798	0.10272795500567064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2873177319006345E-4	0.0
4	0.0	0.0	1.2873177319006345E-4	2.574635463801269E-4	0.0
5	1.2873177319006345E-4	1.2873177319006345E-4	2.574635463801269E-4	2.574635463801269E-4	0.0
6	1.2873177319006345E-4	1.2873177319006345E-4	5.149270927602538E-4	2.574635463801269E-4	0.0
7	1.2873177319006345E-4	1.2873177319006345E-4	0.001673513051470825	5.149270927602538E-4	0.0
8	2.574635463801269E-4	1.2873177319006345E-4	0.002059708371041015	5.149270927602538E-4	0.0
9	2.574635463801269E-4	1.2873177319006345E-4	0.002832099010181396	0.0015447812782807613	0.0
10	5.149270927602538E-4	1.2873177319006345E-4	0.00334702610294165	0.001673513051470825	0.0
11	5.149270927602538E-4	1.2873177319006345E-4	0.005406734473982665	0.001673513051470825	0.0
12	5.149270927602538E-4	1.2873177319006345E-4	0.006565320432693236	0.001673513051470825	0.0
13	5.149270927602538E-4	1.2873177319006345E-4	0.0066940522058833	0.0018022448246608883	0.0
14	5.149270927602538E-4	1.2873177319006345E-4	0.00708024752545349	0.0018022448246608883	0.0
15	5.149270927602538E-4	1.2873177319006345E-4	0.007595174618213744	0.0018022448246608883	0.0
16	5.149270927602538E-4	1.2873177319006345E-4	0.008496297030544189	0.0018022448246608883	0.0
17	5.149270927602538E-4	1.2873177319006345E-4	0.008625028803734251	0.0018022448246608883	0.0
18	6.436588659503173E-4	1.2873177319006345E-4	0.008753760576924315	0.0018022448246608883	0.0
19	6.436588659503173E-4	1.2873177319006345E-4	0.008882492350114379	0.0018022448246608883	0.0
20	6.436588659503173E-4	1.2873177319006345E-4	0.009139955896494505	0.0018022448246608883	0.0
21	6.436588659503173E-4	1.2873177319006345E-4	0.009397419442874632	0.002059708371041015	0.0
22	6.436588659503173E-4	1.2873177319006345E-4	0.009397419442874632	0.0023171719174211423	0.0
23	6.436588659503173E-4	1.2873177319006345E-4	0.009526151216064696	0.0023171719174211423	0.0
24	6.436588659503173E-4	1.2873177319006345E-4	0.009526151216064696	0.002574635463801269	0.0
25	6.436588659503173E-4	1.2873177319006345E-4	0.009526151216064696	0.0027033672369913323	0.0
26	6.436588659503173E-4	1.2873177319006345E-4	0.009783614762444822	0.002832099010181396	0.0
27	6.436588659503173E-4	1.2873177319006345E-4	0.009783614762444822	0.00334702610294165	0.0
28	6.436588659503173E-4	1.2873177319006345E-4	0.009783614762444822	0.010041078308824948	0.0
29	6.436588659503173E-4	2.574635463801269E-4	0.009783614762444822	0.02317171917421142	0.0
30	6.436588659503173E-4	2.574635463801269E-4	0.009783614762444822	0.03861953195701904	0.0
31	6.436588659503173E-4	2.574635463801269E-4	0.009783614762444822	0.08676521513010277	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	595	0.0	27.67227	1
TAAACGA	35	8.866797E-4	26.42857	29
TGTCGAC	35	8.866797E-4	26.42857	36
GTATCAA	1535	0.0	22.296417	1
CCGTAAA	45	0.0038247071	20.555557	26
CGGGCTA	45	0.0038247071	20.555557	16
GCGTATA	45	0.0038247071	20.555557	9
GTAAACG	45	0.0038247071	20.555557	28
TTAACGG	165	0.0	20.181818	35
AGCGTAT	50	0.0070328796	18.5	8
GATGTCG	50	0.0070328796	18.5	34
GATCGAC	50	0.0070328796	18.5	31
ATAAGTC	50	0.0070328796	18.5	3
CGCCGTA	60	9.233142E-4	18.5	24
GCCGTAA	50	0.0070328796	18.5	25
ATCGACA	50	0.0070328796	18.5	32
TACCGGC	50	0.0070328796	18.5	7
TAACGGC	190	0.0	17.526316	36
GTCAACC	170	5.456968E-12	17.411764	15
GTATTAG	335	0.0	16.567163	1
>>END_MODULE