FastQCFastQC Report
Fri 10 Feb 2017
ERR1633183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724749
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51450.7099009450168265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34960.4823738977218319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20780.28671995408065415No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12350.1704038225647776No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11590.1599174334838682No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9640.13301156676311385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.1258366689709127No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8610.11879974998240769No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8380.11562623749739566No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7770.1072095304719289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA250.005495019329.64
TACATCG358.86622E-426.428575
GTATAGA1000.025.9000021
GTATCAA27650.023.8860761
ACATCGC551.901402E-523.5454546
GGTATCA10050.023.3781091
CCGTTTA957.1340764E-921.42105327
TATACAG1059.804353E-1021.1428595
GGACCGT1251.8189894E-1120.726
CGCCGTT909.466203E-820.55555725
TTACCGG759.258025E-619.73333431
ATAGAAC951.6739796E-719.4736843
TCTAGAC500.007032433518.53
CGCCGTC1409.458745E-1118.526
CCGCCGT1002.8712748E-718.524
TACCGGG902.1499836E-618.532
TGCTCGC2350.018.10638210
GTACTGG3500.017.971431
AGGACCG1351.1496013E-917.8148165
GACCGTT1054.791218E-717.619057
Produced by FastQC (version 0.11.5)