##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633183.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724749
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84890355143643	33.0	33.0	33.0	33.0	33.0
2	31.9695908514534	33.0	33.0	33.0	33.0	33.0
3	31.69454114458937	33.0	33.0	33.0	27.0	33.0
4	31.97759224227974	33.0	33.0	33.0	33.0	33.0
5	32.07313704468719	33.0	33.0	33.0	33.0	33.0
6	35.21865501021733	37.0	37.0	37.0	33.0	37.0
7	35.36345410618021	37.0	37.0	37.0	33.0	37.0
8	35.498941012681634	37.0	37.0	37.0	33.0	37.0
9	35.61945997855809	37.0	37.0	37.0	33.0	37.0
10	35.606284382593145	37.0	37.0	37.0	33.0	37.0
11	35.639912576629975	37.0	37.0	37.0	33.0	37.0
12	35.61991254903422	37.0	37.0	37.0	33.0	37.0
13	35.62025059710327	37.0	37.0	37.0	33.0	37.0
14	35.603760750273544	37.0	37.0	37.0	33.0	37.0
15	35.61962555312253	37.0	37.0	37.0	33.0	37.0
16	35.61251826494414	37.0	37.0	37.0	33.0	37.0
17	35.590063594430625	37.0	37.0	37.0	33.0	37.0
18	35.58001459815743	37.0	37.0	37.0	33.0	37.0
19	35.56689695329003	37.0	37.0	37.0	33.0	37.0
20	35.56702941294159	37.0	37.0	37.0	33.0	37.0
21	35.57065825547879	37.0	37.0	37.0	33.0	37.0
22	35.46905756337711	37.0	37.0	37.0	33.0	37.0
23	35.51666852938052	37.0	37.0	37.0	33.0	37.0
24	35.51986825783823	37.0	37.0	37.0	33.0	37.0
25	35.54009733024813	37.0	37.0	37.0	33.0	37.0
26	35.43007855133295	37.0	37.0	37.0	33.0	37.0
27	35.43194678433499	37.0	37.0	37.0	33.0	37.0
28	35.452257264239066	37.0	37.0	37.0	33.0	37.0
29	35.46068500956883	37.0	37.0	37.0	33.0	37.0
30	35.46359636232682	37.0	37.0	37.0	33.0	37.0
31	35.45634833576866	37.0	37.0	37.0	33.0	37.0
32	35.44635591080498	37.0	37.0	37.0	33.0	37.0
33	35.438561488184185	37.0	37.0	37.0	33.0	37.0
34	35.406557304666855	37.0	37.0	37.0	33.0	37.0
35	35.34795908652513	37.0	37.0	37.0	33.0	37.0
36	35.3612326474407	37.0	37.0	37.0	33.0	37.0
37	35.359408567655834	37.0	37.0	37.0	33.0	37.0
38	35.34008601598622	37.0	37.0	37.0	33.0	37.0
39	35.241948591857316	37.0	37.0	37.0	33.0	37.0
40	35.02894243386331	37.0	37.0	37.0	27.0	37.0
41	35.18916066113923	37.0	37.0	37.0	33.0	37.0
42	35.22269778916563	37.0	37.0	37.0	33.0	37.0
43	34.87814677909179	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	25.0
16	36.0
17	32.0
18	31.0
19	21.0
20	36.0
21	114.0
22	330.0
23	831.0
24	1619.0
25	3022.0
26	4883.0
27	7445.0
28	10313.0
29	14022.0
30	18133.0
31	23062.0
32	29583.0
33	38401.0
34	55248.0
35	111343.0
36	406215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77108350615178	19.63769525725458	13.72709724332148	23.864123993272155
2	16.241071046665812	21.545804133569003	39.14306884176453	23.07005597800066
3	18.498404275135254	27.363818370221964	29.867029826877996	24.270747527764787
4	12.345929418322758	17.744074155328256	39.63620508617466	30.273791340174323
5	14.247967227274547	36.53250987583287	34.453721219346285	14.765801677546294
6	29.146504513976563	39.80412529027291	17.15863009124538	13.890740104505145
7	26.66371392026757	32.55568479570169	22.503239052416767	18.27736223161398
8	24.945049941428	34.800185995427384	20.172363121577263	20.082400941567357
9	26.713938204812976	15.010852032910705	19.827002175925735	38.448207586350584
10	15.68722412862936	27.402176477649505	32.743887883943266	24.16671150977787
11	34.41136172661155	23.85418951940603	23.355948059259138	18.378500694723275
12	23.801619595197785	25.426871923935046	29.718426655297215	21.053081825569954
13	29.237294566808647	22.259430506285625	25.556434020605757	22.946840906299972
14	22.710345236764727	21.010998290442622	27.990794054217393	28.28786241857526
15	24.68661564210506	27.691656007804085	24.94642972946496	22.6752986206259
16	22.46639871182989	28.029980034467105	25.31345334729679	24.190167906406217
17	22.670469362496533	26.749122799755504	26.008728539121822	24.571679298626144
18	24.095376468266945	25.60031128018114	27.679927809489907	22.62438444206201
19	25.88344378536569	25.403829463717788	27.546088369904613	21.16663838101191
20	25.326147397236838	24.978026875511382	27.45695406271689	22.238871664534894
21	23.645703547021107	26.24508622985337	27.685308982834055	22.423901240291467
22	23.27219492541556	26.086824542013854	27.588999777854127	23.05198075471646
23	23.13669974018591	26.054399523145257	28.310628921185128	22.498271815483704
24	23.182784660620438	26.24149878095727	27.22611552413318	23.349601034289115
25	23.247910655965033	26.243844420620103	28.161749791996954	22.346495131417914
26	23.55601732461859	26.259849961848865	27.47047598547911	22.713656728053436
27	23.642530034536094	26.11221264189395	27.421907446578057	22.823349876991898
28	22.809276039014886	26.20038109745581	27.7776168025068	23.212726061022508
29	24.029974515314958	25.99548257396699	27.06523223902344	22.909310671694612
30	23.796928315872115	25.762022438113057	27.88868973948222	22.552359506532607
31	23.64984291113199	25.75719317998369	27.454470444250354	23.138493464633964
32	22.265777531255647	25.972026177338638	28.05288451588067	23.709311775525045
33	22.445977848882855	26.007762687495944	28.428186861934268	23.11807260168693
34	22.928351746604687	25.89075666196159	27.824667574567197	23.356224016866527
35	22.864605539297052	26.503382550372613	27.70007271482955	22.931939195500785
36	23.597410965727445	25.962091703472513	27.563197741562938	22.8772995892371
37	23.48068089780048	25.825492687813302	27.4059019053493	23.287924509036923
38	23.292063873147807	26.21293716859216	27.063990429790174	23.431008528469857
39	22.987682632194044	25.834599288857245	27.64322544770672	23.53449263124199
40	23.23287096636215	26.524631286141826	27.67551248777163	22.5669852597244
41	23.39513403950885	25.59424021281851	27.630255440159285	23.38037030751336
42	22.530696834352305	26.576511316331587	27.73939667388296	23.15339517543315
43	23.32407495560532	26.001553641329618	27.287240134170588	23.387131268894475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	45.5
2	71.0
3	265.0
4	459.0
5	459.0
6	573.5
7	688.0
8	668.0
9	648.0
10	895.5
11	1143.0
12	1143.0
13	1813.0
14	2483.0
15	4366.0
16	6249.0
17	7137.0
18	8025.0
19	8025.0
20	7055.5
21	6086.0
22	5997.0
23	5908.0
24	7268.5
25	8629.0
26	8629.0
27	10775.0
28	12921.0
29	15378.5
30	17836.0
31	20896.5
32	23957.0
33	23957.0
34	28570.5
35	33184.0
36	35590.0
37	37996.0
38	41038.5
39	44081.0
40	44081.0
41	46889.0
42	49697.0
43	51672.5
44	53648.0
45	54827.0
46	56006.0
47	56006.0
48	56746.5
49	57487.0
50	57642.0
51	57797.0
52	56939.0
53	56081.0
54	56081.0
55	52530.5
56	48980.0
57	43532.5
58	38085.0
59	34880.5
60	31676.0
61	31676.0
62	27368.0
63	23060.0
64	18668.0
65	14276.0
66	12363.5
67	10451.0
68	10451.0
69	8637.0
70	6823.0
71	5553.0
72	4283.0
73	3500.0
74	2717.0
75	2717.0
76	2030.5
77	1344.0
78	1042.5
79	741.0
80	609.5
81	478.0
82	478.0
83	400.0
84	322.0
85	267.0
86	212.0
87	167.0
88	122.0
89	122.0
90	92.0
91	62.0
92	38.5
93	15.0
94	8.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	724749.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.47017065835113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.64319769026574	49.21929186986475
2	8.337016841414664	9.582595613152964
3	2.193662549666558	3.7821048328851212
4	0.9792938656914855	2.2512074234386423
5	0.5923410133631182	1.7020969562959523
6	0.36618246069526883	1.2626741104951231
7	0.2769825973624401	1.114276599786895
8	0.2027642471305066	0.9322316708801835
9	0.16553385085999123	0.8561932792791965
>10	1.0340119228650004	11.65830340643574
>50	0.1230205357045738	4.939485936218687
>100	0.08038951910375507	9.20093876926169
>500	0.004384882860170561	1.662779777575996
>1k	9.744184133712357E-4	1.1155198507809663
>5k	2.4360460334280892E-4	0.7202999036481014
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5145	0.7099009450168265	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3496	0.4823738977218319	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2078	0.28671995408065415	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1235	0.1704038225647776	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1159	0.1599174334838682	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	964	0.13301156676311385	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	912	0.1258366689709127	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	861	0.11879974998240769	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	838	0.11562623749739566	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	777	0.1072095304719289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.379788036961762E-4	0.0	0.0
2	1.379788036961762E-4	0.0	2.759576073923524E-4	0.0	0.0
3	1.379788036961762E-4	0.0	4.1393641108852856E-4	0.0	0.0
4	1.379788036961762E-4	0.0	5.519152147847048E-4	2.759576073923524E-4	0.0
5	1.379788036961762E-4	0.0	9.658516258732333E-4	2.759576073923524E-4	0.0
6	1.379788036961762E-4	0.0	0.0017937244480502905	4.1393641108852856E-4	0.0
7	1.379788036961762E-4	0.0	0.0038634065034929333	6.898940184808809E-4	0.0
8	1.379788036961762E-4	0.0	0.004139364110885286	6.898940184808809E-4	0.0
9	1.379788036961762E-4	0.0	0.005657130951543224	0.0022076608591388193	0.0
10	4.1393641108852856E-4	0.0	0.0057951097552394	0.0022076608591388193	0.0
11	4.1393641108852856E-4	0.0	0.009796495062428509	0.0026215972702273477	0.0
12	5.519152147847048E-4	0.0	0.010762346688301743	0.0027595760739235237	0.0
13	5.519152147847048E-4	0.0	0.0115902195104788	0.0028975548776197	0.0
14	5.519152147847048E-4	0.0	0.01228011352895968	0.0028975548776197	0.0
15	5.519152147847048E-4	0.0	0.013659901565921444	0.0028975548776197	0.0
16	5.519152147847048E-4	0.0	0.015039689602883205	0.003035533681315876	0.0
17	5.519152147847048E-4	0.0	0.01559160481766791	0.003035533681315876	0.0
18	5.519152147847048E-4	0.0	0.015867562425060263	0.003035533681315876	0.0
19	5.519152147847048E-4	1.379788036961762E-4	0.01628149883614879	0.003035533681315876	0.0
20	5.519152147847048E-4	1.379788036961762E-4	0.01697139285462967	0.0033114912887082285	0.0
21	5.519152147847048E-4	1.379788036961762E-4	0.0173853292657182	0.0038634065034929333	1.379788036961762E-4
22	5.519152147847048E-4	1.379788036961762E-4	0.017661286873110554	0.0049672369330623425	1.379788036961762E-4
23	5.519152147847048E-4	1.379788036961762E-4	0.018075223284199082	0.005105215736758519	1.379788036961762E-4
24	5.519152147847048E-4	2.759576073923524E-4	0.018075223284199082	0.006071067362631752	1.379788036961762E-4
25	5.519152147847048E-4	2.759576073923524E-4	0.018351180891591434	0.006760961381112634	1.379788036961762E-4
26	5.519152147847048E-4	2.759576073923524E-4	0.018351180891591434	0.00800277061437822	1.379788036961762E-4
27	5.519152147847048E-4	2.759576073923524E-4	0.018351180891591434	0.009244579847643805	2.759576073923524E-4
28	5.519152147847048E-4	2.759576073923524E-4	0.018351180891591434	0.01917905371376849	2.759576073923524E-4
29	5.519152147847048E-4	2.759576073923524E-4	0.018351180891591434	0.03904800144601786	2.759576073923524E-4
30	5.519152147847048E-4	2.759576073923524E-4	0.018351180891591434	0.06857546543699956	2.759576073923524E-4
31	5.519152147847048E-4	2.759576073923524E-4	0.018351180891591434	0.1303899694928865	2.759576073923524E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	25	0.0054950193	29.6	4
TACATCG	35	8.86622E-4	26.42857	5
GTATAGA	100	0.0	25.900002	1
GTATCAA	2765	0.0	23.886076	1
ACATCGC	55	1.901402E-5	23.545454	6
GGTATCA	1005	0.0	23.378109	1
CCGTTTA	95	7.1340764E-9	21.421053	27
TATACAG	105	9.804353E-10	21.142859	5
GGACCGT	125	1.8189894E-11	20.72	6
CGCCGTT	90	9.466203E-8	20.555557	25
TTACCGG	75	9.258025E-6	19.733334	31
ATAGAAC	95	1.6739796E-7	19.473684	3
TCTAGAC	50	0.0070324335	18.5	3
CGCCGTC	140	9.458745E-11	18.5	26
CCGCCGT	100	2.8712748E-7	18.5	24
TACCGGG	90	2.1499836E-6	18.5	32
TGCTCGC	235	0.0	18.106382	10
GTACTGG	350	0.0	17.97143	1
AGGACCG	135	1.1496013E-9	17.814816	5
GACCGTT	105	4.791218E-7	17.61905	7
>>END_MODULE