FastQCFastQC Report
Fri 10 Feb 2017
ERR1633182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678991
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27800.409431052841643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.28365618984640445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11130.16391969849379448No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9170.13505333649488727No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8580.12636397242378763No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7740.11399267442425598No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7340.10810158013876472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.027.8348621
CGGTAAT358.86564E-426.42857224
GCGGTAA451.3224331E-424.66666623
TATCCCG400.001930377823.12510
GTATCAA16000.022.5468771
AACGATT753.736095E-722.222
CACGGAA450.003824219820.55555525
TAGGACC1551.8189894E-1219.0967754
GTATCGA500.007031989318.516
CGCACCT701.21826466E-418.529
CGCCGTC701.21826466E-418.526
ATTTACG650.001579033617.07692327
CGAGTCG650.001579033617.07692321
TCGGTAG650.001579033617.07692328
GTACGAT650.001579033617.0769236
TAACTAG1757.2759576E-1216.91428626
GTATTAG2750.016.8181821
ATCAACG21200.016.4929263
TTCAACG904.441646E-516.44444519
TCTATAC1352.2144377E-816.4444433
Produced by FastQC (version 0.11.5)