##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633182.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	678991
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70935108123672	33.0	33.0	33.0	27.0	33.0
2	31.865541664027948	33.0	33.0	33.0	33.0	33.0
3	31.590705915100493	33.0	33.0	33.0	27.0	33.0
4	31.882432904118023	33.0	33.0	33.0	33.0	33.0
5	31.99013094429823	33.0	33.0	33.0	33.0	33.0
6	35.09878039620555	37.0	37.0	37.0	33.0	37.0
7	35.255720620744604	37.0	37.0	37.0	33.0	37.0
8	35.37088267738453	37.0	37.0	37.0	33.0	37.0
9	35.509398504545715	37.0	37.0	37.0	33.0	37.0
10	35.51529696269906	37.0	37.0	37.0	33.0	37.0
11	35.542540328222316	37.0	37.0	37.0	33.0	37.0
12	35.51405688735197	37.0	37.0	37.0	33.0	37.0
13	35.52151059439669	37.0	37.0	37.0	33.0	37.0
14	35.50460904489161	37.0	37.0	37.0	33.0	37.0
15	35.51231459621703	37.0	37.0	37.0	33.0	37.0
16	35.49475913524627	37.0	37.0	37.0	33.0	37.0
17	35.48823769387223	37.0	37.0	37.0	33.0	37.0
18	35.470118160623635	37.0	37.0	37.0	33.0	37.0
19	35.46511662157525	37.0	37.0	37.0	33.0	37.0
20	35.46515785923525	37.0	37.0	37.0	33.0	37.0
21	35.46290451567105	37.0	37.0	37.0	33.0	37.0
22	35.374424697823684	37.0	37.0	37.0	33.0	37.0
23	35.42559327001389	37.0	37.0	37.0	33.0	37.0
24	35.410316189758035	37.0	37.0	37.0	33.0	37.0
25	35.432127966350066	37.0	37.0	37.0	33.0	37.0
26	35.314565288788806	37.0	37.0	37.0	33.0	37.0
27	35.32600579389123	37.0	37.0	37.0	33.0	37.0
28	35.34700018115115	37.0	37.0	37.0	33.0	37.0
29	35.34477923860552	37.0	37.0	37.0	33.0	37.0
30	35.339313775882154	37.0	37.0	37.0	33.0	37.0
31	35.334079538609494	37.0	37.0	37.0	33.0	37.0
32	35.32395863862702	37.0	37.0	37.0	33.0	37.0
33	35.329359299313246	37.0	37.0	37.0	33.0	37.0
34	35.31523098244307	37.0	37.0	37.0	33.0	37.0
35	35.24745541546206	37.0	37.0	37.0	33.0	37.0
36	35.276932978493086	37.0	37.0	37.0	33.0	37.0
37	35.273532344316784	37.0	37.0	37.0	33.0	37.0
38	35.26819501289413	37.0	37.0	37.0	33.0	37.0
39	35.16451322624306	37.0	37.0	37.0	33.0	37.0
40	34.95726158373233	37.0	37.0	37.0	27.0	37.0
41	35.126649690496635	37.0	37.0	37.0	27.0	37.0
42	35.16847793269719	37.0	37.0	37.0	33.0	37.0
43	34.81367499716491	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	25.0
16	44.0
17	40.0
18	33.0
19	21.0
20	57.0
21	164.0
22	403.0
23	919.0
24	1896.0
25	3187.0
26	5173.0
27	7601.0
28	10486.0
29	13980.0
30	17983.0
31	22787.0
32	28765.0
33	37722.0
34	54072.0
35	108009.0
36	365620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41454010436074	20.261093298732973	13.6411233727693	24.68324322413699
2	16.288728422026214	21.33400884547807	38.83497719410125	23.54228553839447
3	17.929103625821256	27.176501603114033	29.62160028630718	25.272794484757533
4	12.55377464502475	17.769896802755856	39.36738484015252	30.308943712066878
5	14.042306893611256	36.77927984317907	35.1340444865985	14.044368776611178
6	29.83235418437063	39.53395553107479	17.119667270994757	13.514023013559825
7	27.114645113116374	31.89364807486403	23.211500594264137	17.780206217755463
8	24.792375745775715	35.71947198121919	20.168750395807898	19.319401877197194
9	26.19327796686554	15.367655830489655	19.963593037315665	38.47547316532914
10	15.4974071821276	27.651176525167493	33.27614062631169	23.57527566639322
11	34.35479998998514	23.446997088326647	23.721374804673406	18.476828117014808
12	23.53300706489482	25.600781159102254	29.645753772877697	21.220458003125227
13	28.189327988147117	22.08335603859256	25.7814904763097	23.945825496950622
14	22.26922006329981	20.758890765856986	27.589761867241247	29.382127303601962
15	24.345683521578344	28.294336743786	24.353194666792344	23.006785067843314
16	23.23683229969175	27.937041875370955	24.974410559197395	23.8517152657399
17	23.02107097148563	27.06353987018974	25.833184828664884	24.082204329659746
18	23.38690792661464	26.920680833766575	27.441895400675413	22.250515838943375
19	25.31152842968464	25.818604370308297	27.035409894976514	21.834457305030554
20	24.630370652924707	25.82596823816516	27.179152595542504	22.36450851336763
21	23.499280550110385	26.530543114709914	27.265309849467812	22.704866485711886
22	23.0746799294836	26.44364947399892	27.163983027757364	23.317687568760114
23	23.120630464910434	26.3147817865038	27.713327569879425	22.851260178706344
24	23.28999942561831	26.946307093908462	26.2962248395045	23.46746864096873
25	23.587646964392754	26.451013341855784	27.3517616581074	22.609578035644066
26	23.501636987824583	26.730693043059482	27.11597060933061	22.65169935978533
27	23.367614592829653	26.778558184129096	26.54394535420941	23.30988186883184
28	22.757444502209896	26.434960109927818	27.58033611638446	23.227259271477827
29	24.439204643360515	26.276784228362377	26.478406930283317	22.805604197993787
30	23.718281980173522	26.128623207082274	27.164719414543047	22.988375398201157
31	23.574244724893262	26.08178900751262	27.164719414543047	23.179246853051072
32	22.557294573860332	26.092392977226503	27.4327642045329	23.917548244380267
33	22.577471571788138	26.282380767933596	27.928941620728402	23.21120603954986
34	23.37100197204381	26.064263002013284	27.426873110247413	23.137861915695495
35	23.274682580476032	26.78268195012894	26.875613962482564	23.067021506912464
36	24.009743869948203	26.317727333646545	26.70580316970328	22.966725626701972
37	23.49250579168207	25.821402640093904	27.046308419404674	23.63978314881935
38	23.44478792796959	26.448067794713037	26.867366430482882	23.239777846834496
39	23.159364409837536	26.208889366722094	27.18857834639929	23.44316787704108
40	23.17129387576566	26.762504952201134	27.338064863893628	22.728136308139575
41	23.175123087051226	25.809325896808648	27.239094479897375	23.776456536242748
42	22.709432083783142	26.872815692696957	27.25794598161095	23.15980624190895
43	23.60414202839213	25.861903913306655	26.930401139337633	23.60355291896358
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	31.0
2	48.0
3	171.0
4	294.0
5	294.0
6	345.0
7	396.0
8	379.0
9	362.0
10	489.0
11	616.0
12	616.0
13	952.5
14	1289.0
15	2357.5
16	3426.0
17	4025.5
18	4625.0
19	4625.0
20	4222.5
21	3820.0
22	3996.5
23	4173.0
24	5478.5
25	6784.0
26	6784.0
27	8935.0
28	11086.0
29	13783.0
30	16480.0
31	19964.5
32	23449.0
33	23449.0
34	29063.5
35	34678.0
36	37690.0
37	40702.0
38	44081.0
39	47460.0
40	47460.0
41	50193.0
42	52926.0
43	54558.0
44	56190.0
45	55292.5
46	54395.0
47	54395.0
48	53229.0
49	52063.0
50	52232.0
51	52401.0
52	49908.5
53	47416.0
54	47416.0
55	45496.5
56	43577.0
57	37838.0
58	32099.0
59	29626.0
60	27153.0
61	27153.0
62	24020.5
63	20888.0
64	17240.5
65	13593.0
66	11630.0
67	9667.0
68	9667.0
69	8097.0
70	6527.0
71	5385.0
72	4243.0
73	3399.0
74	2555.0
75	2555.0
76	1981.0
77	1407.0
78	1123.0
79	839.0
80	706.5
81	574.0
82	574.0
83	457.0
84	340.0
85	289.0
86	238.0
87	177.0
88	116.0
89	116.0
90	92.0
91	68.0
92	39.0
93	10.0
94	7.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	678991.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.66912626557533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.86019637840178	48.089531834891694
2	8.356624284480791	9.4712519346243
3	2.411120517176539	4.099082790881897
4	1.172895725805761	2.6586790392816115
5	0.6368039123916027	1.8043560658866056
6	0.4385460860119924	1.4911214112892475
7	0.3119911096984865	1.2376184512468347
8	0.24338491443359114	1.103392835773874
9	0.1860688123307944	0.9489921318053485
>10	1.1609625533720958	12.699115760984487
>50	0.13268550064033405	5.218065464652649
>100	0.08319164647168067	8.642565550568824
>500	0.0047387646724313965	1.6680912380604045
>1k	7.897941120718995E-4	0.8681354900521863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2780	0.409431052841643	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1926	0.28365618984640445	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.16391969849379448	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	917	0.13505333649488727	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	858	0.12636397242378763	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	774	0.11399267442425598	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	734	0.10810158013876472	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.945547142745633E-4	0.0	0.0
2	0.0	0.0	2.945547142745633E-4	0.0	0.0
3	0.0	0.0	5.891094285491266E-4	1.4727735713728165E-4	0.0
4	0.0	0.0	5.891094285491266E-4	2.945547142745633E-4	0.0
5	0.0	0.0	0.0010309414999609716	2.945547142745633E-4	0.0
6	0.0	0.0	0.0014727735713728164	2.945547142745633E-4	0.0
7	0.0	0.0	0.003681933928432041	2.945547142745633E-4	0.0
8	1.4727735713728165E-4	0.0	0.003976488642706604	2.945547142745633E-4	0.0
9	1.4727735713728165E-4	0.0	0.004565598071255731	8.836641428236899E-4	0.0
10	1.4727735713728165E-4	0.0	0.005301984856942139	8.836641428236899E-4	0.0
11	1.4727735713728165E-4	0.0	0.007069313142589519	8.836641428236899E-4	0.0
12	1.4727735713728165E-4	0.0	0.007511145214001364	8.836641428236899E-4	0.0
13	1.4727735713728165E-4	1.4727735713728165E-4	0.008247531999687772	8.836641428236899E-4	0.0
14	1.4727735713728165E-4	1.4727735713728165E-4	0.008836641428236898	0.0010309414999609716	0.0
15	1.4727735713728165E-4	1.4727735713728165E-4	0.009573028213923307	0.0010309414999609716	0.0
16	1.4727735713728165E-4	1.4727735713728165E-4	0.010309414999609716	0.0010309414999609716	0.0
17	1.4727735713728165E-4	1.4727735713728165E-4	0.010456692356746997	0.0010309414999609716	0.0
18	1.4727735713728165E-4	1.4727735713728165E-4	0.010456692356746997	0.0011782188570982532	0.0
19	1.4727735713728165E-4	1.4727735713728165E-4	0.01075124707102156	0.0013254962142355347	0.0
20	1.4727735713728165E-4	1.4727735713728165E-4	0.010898524428158841	0.0014727735713728164	0.0
21	1.4727735713728165E-4	1.4727735713728165E-4	0.01163491121384525	0.001620050928510098	0.0
22	1.4727735713728165E-4	1.4727735713728165E-4	0.011782188570982531	0.001620050928510098	0.0
23	1.4727735713728165E-4	2.945547142745633E-4	0.011929465928119814	0.0023564377141965065	0.0
24	1.4727735713728165E-4	2.945547142745633E-4	0.011929465928119814	0.0030928244998829147	0.0
25	1.4727735713728165E-4	2.945547142745633E-4	0.012076743285257095	0.0030928244998829147	0.0
26	1.4727735713728165E-4	2.945547142745633E-4	0.012076743285257095	0.004123765999843886	0.0
27	1.4727735713728165E-4	2.945547142745633E-4	0.012224020642394376	0.004860152785530294	0.0
28	1.4727735713728165E-4	2.945547142745633E-4	0.012224020642394376	0.011487633856707967	0.0
29	1.4727735713728165E-4	2.945547142745633E-4	0.012224020642394376	0.02960274878459361	0.0
30	1.4727735713728165E-4	2.945547142745633E-4	0.012224020642394376	0.05508173156934333	0.0
31	1.4727735713728165E-4	2.945547142745633E-4	0.012224020642394376	0.10574514242456821	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	545	0.0	27.834862	1
CGGTAAT	35	8.86564E-4	26.428572	24
GCGGTAA	45	1.3224331E-4	24.666666	23
TATCCCG	40	0.0019303778	23.125	10
GTATCAA	1600	0.0	22.546877	1
AACGATT	75	3.736095E-7	22.2	22
CACGGAA	45	0.0038242198	20.555555	25
TAGGACC	155	1.8189894E-12	19.096775	4
GTATCGA	50	0.0070319893	18.5	16
CGCACCT	70	1.21826466E-4	18.5	29
CGCCGTC	70	1.21826466E-4	18.5	26
ATTTACG	65	0.0015790336	17.076923	27
CGAGTCG	65	0.0015790336	17.076923	21
TCGGTAG	65	0.0015790336	17.076923	28
GTACGAT	65	0.0015790336	17.076923	6
TAACTAG	175	7.2759576E-12	16.914286	26
GTATTAG	275	0.0	16.818182	1
ATCAACG	2120	0.0	16.492926	3
TTCAACG	90	4.441646E-5	16.444445	19
TCTATAC	135	2.2144377E-8	16.444443	3
>>END_MODULE