FastQCFastQC Report
Fri 10 Feb 2017
ERR1633181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654198
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT173392.650420820607828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT127131.9432954548928614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66751.020333293590014No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA39020.5964555073540426No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA32340.49434574853484725No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31510.4816584581426418No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG30780.4704997569543166No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG29220.4466537653737859No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT27290.4171519937388986No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23700.3622756413196005No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC23680.36196992347882445No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG19950.3049535461740941No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19280.29471199850809693No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18150.277438940504251No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.27132458368873036No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA17270.2639873555101055No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17250.2636816376693295No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16950.25909587005768897No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC16440.2513000651179001No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC16180.24732573318781165No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC16140.24671429750625956No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC15040.2298998162635777No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15020.22959409842280165No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14890.22760693245775743No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14620.22347974160728096No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC14420.22042256319952064No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14110.2156839366674921No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14060.21491964206555203No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13940.2130853350208958No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC13350.2040666587180028No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13330.2037609408772268No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13300.20330236411606273No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12890.197035148380154No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12800.19565941809666187No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA12480.19076793264424532No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT12340.18862790775881308No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG12110.18511215258988867No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11970.18297212770445645No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11900.18190211526174033No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11760.17976209037630808No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11720.17915065469475602No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11690.178692077933592No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT11510.17594061736660765No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11320.17303629787923533No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT10960.16753337674526672No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT10610.16218331453168613No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10580.16172473777052207No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10530.160960443168582No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10500.16050186640741795No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.15882041828314974No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10380.15866755936276175No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10120.1546932274326733No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10020.15316463822879312No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA9990.15270606146762905No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9760.14919030629870467No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9740.14888458845792865No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9720.1485788706171526No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9700.14827315277637657No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9610.14689742249288443No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9540.1458274100501683No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9520.14552169220939226No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG9460.14460453868706416No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9450.14445167976667614No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA9370.14322880840357202No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA9210.14078306567736373No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG9210.14078306567736373No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9170.14017162999581168No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA9150.13986591215503563No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9040.13818446403076745No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA9040.13818446403076745No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG9000.13757302834921536No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8910.13619729806572323No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.13482156778223106No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.13405727318029098No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8710.13314011965796288No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8610.13161153045408272No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8560.13084723585214264No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8430.12886006988709842No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8140.1244271611958459No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8110.12396858443468185No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG8110.12396858443468185No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG7860.12014711142498143No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7730.1181599454599372No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7600.11617277949489298No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC7500.11464419029101282No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7460.11403275460946075No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7450.11387989568907274No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC7430.1135741778482967No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7390.11296274216674462No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG7290.11143415296286446No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7260.1109755762017004No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7190.10990556375898428No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG7170.10959984591820825No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG7130.10898841023665617No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7040.10761267995316402No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT6970.1065426675104479No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6910.10562551398811981No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA6840.10455550154540368No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6820.10424978370462766No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6770.10348548910268758No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6700.10241547665997144No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6690.10226261773958344No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6660.10180404097841937No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6580.10058116961531524No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6570.10042831069492722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGA200.001841356237.09
ATGTTCC200.001841356237.021
TGGAACC303.5977978E-430.83333424
CTAGTCG303.5977978E-430.8333349
TGTCAAC250.00549463329.62
AGCAACG250.00549463329.62
TACCCTA250.00549463329.612
AGTCCAA250.00549463329.611
TAGACCG250.00549463329.65
CGTGAAA250.00549463329.611
TAGCAAC250.00549463329.61
AAGCCCT250.00549463329.637
ACGTGAA250.00549463329.610
GATATTC250.00549463329.63
AAATTAG250.00549463329.629
ACCAACG250.00549463329.62
GTCCAAA250.00549463329.612
GACTGGT250.00549463329.633
TATACGC250.00549463329.64
AAAATTA250.00549463329.628
Produced by FastQC (version 0.11.5)