FastQCFastQC Report
Fri 10 Feb 2017
ERR1633180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716650
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63070.880066978301821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44170.6163399148817414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23910.3336356659457197No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18980.2648433684504291No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC17790.24823833112397964No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG17360.24223819158585083No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC17110.23874973836600852No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15370.21447010395590593No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15010.20944673131933303No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA14960.20874904067536454No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC13620.19005093141700968No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT13550.18907416451545386No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG13430.18739970696992952No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13230.18460894439405567No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC13180.1839112537500872No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT12800.17860880485592687No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG12320.17191097467382963No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12160.16967836461313054No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA12020.16772483081001885No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12000.16744575455243146No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11730.16367822507500174No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG11670.16284099630223958No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG11550.16116653875671527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.14930579780925138No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10510.14665457336217122No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10440.14567780646061537No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC10320.14400334891509103No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10010.13967766692248657No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG9990.13939859066489918No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT9770.13632875183143794No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9770.13632875183143794No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC9740.13591013744505687No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG9620.13423567989953256No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC9500.13256122235400825No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG9410.13130537919486499No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA9410.13130537919486499No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT9410.13130537919486499No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC9370.13074722667969024No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9340.13032861229330917No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9100.12697969720226052No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG8710.1215377101793065No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8680.12111909579292542No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT8590.11986325263378218No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8500.11860740947463894No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA8190.11428172748203447No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA8010.11177004116374799No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7950.11093281239098585No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG7890.11009558361822368No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7830.10925835484546154No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC7740.10800251168631829No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC7580.1057699016256192No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7550.10535128723923812No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT7420.10353729156492011No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7310.1020023721481895No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT7300.1018628340193958No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC7270.10144421963301471No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7250.10116514337542733No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7180.1001883764738715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAATC250.005494980629.5999982
CCTGTAG250.005494980629.59999810
CTTGGGA250.005494980629.5999983
TTGACAA405.9370563E-527.75000234
GGTATCA10100.027.2920781
CGCTCTC1600.026.59375229
AACAAGA358.8661246E-426.4285713
CCCAACG358.8661246E-426.4285721
CCGCTCT1700.025.02941128
GCTCTCT1700.025.02941130
GACAATC451.3225328E-424.66666836
CTAGTGC451.3225328E-424.6666689
ATATACA751.3718818E-824.6666661
GTATCAA31350.024.1945781
GCCGCTC1700.023.94117527
CGTTATT701.9208164E-723.78571310
TATACAA701.9208164E-723.7857132
TTATTGT701.9208164E-723.78571312
CCGTCAA551.9013632E-523.54545424
CCCCGTC551.9013632E-523.54545422
Produced by FastQC (version 0.11.5)