##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	716650
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.856798995325473	33.0	33.0	33.0	27.0	33.0
2	31.963334961278168	33.0	33.0	33.0	33.0	33.0
3	31.689268122514477	33.0	33.0	33.0	27.0	33.0
4	31.971334682201913	33.0	33.0	33.0	33.0	33.0
5	32.083315425940135	33.0	33.0	33.0	33.0	33.0
6	35.20539175329659	37.0	37.0	37.0	33.0	37.0
7	35.355308728109954	37.0	37.0	37.0	33.0	37.0
8	35.48969231842601	37.0	37.0	37.0	33.0	37.0
9	35.58662247959255	37.0	37.0	37.0	33.0	37.0
10	35.5724119165562	37.0	37.0	37.0	33.0	37.0
11	35.61081001883765	37.0	37.0	37.0	33.0	37.0
12	35.57361054908254	37.0	37.0	37.0	33.0	37.0
13	35.5871248168562	37.0	37.0	37.0	33.0	37.0
14	35.569369985348494	37.0	37.0	37.0	33.0	37.0
15	35.585719667899255	37.0	37.0	37.0	33.0	37.0
16	35.571726784343824	37.0	37.0	37.0	33.0	37.0
17	35.55846926672713	37.0	37.0	37.0	33.0	37.0
18	35.55213702644247	37.0	37.0	37.0	33.0	37.0
19	35.53677527384358	37.0	37.0	37.0	33.0	37.0
20	35.53795576641317	37.0	37.0	37.0	33.0	37.0
21	35.53255982697272	37.0	37.0	37.0	33.0	37.0
22	35.43349333705435	37.0	37.0	37.0	33.0	37.0
23	35.489499755808275	37.0	37.0	37.0	33.0	37.0
24	35.47277611107235	37.0	37.0	37.0	33.0	37.0
25	35.494524523826136	37.0	37.0	37.0	33.0	37.0
26	35.38405358264146	37.0	37.0	37.0	33.0	37.0
27	35.39647526686667	37.0	37.0	37.0	33.0	37.0
28	35.41485383381009	37.0	37.0	37.0	33.0	37.0
29	35.425171283053096	37.0	37.0	37.0	33.0	37.0
30	35.41119235331054	37.0	37.0	37.0	33.0	37.0
31	35.378040884671734	37.0	37.0	37.0	33.0	37.0
32	35.373171003976836	37.0	37.0	37.0	33.0	37.0
33	35.37163747994139	37.0	37.0	37.0	33.0	37.0
34	35.35818460894439	37.0	37.0	37.0	33.0	37.0
35	35.264068931835624	37.0	37.0	37.0	33.0	37.0
36	35.29534082187958	37.0	37.0	37.0	33.0	37.0
37	35.28429498360427	37.0	37.0	37.0	33.0	37.0
38	35.259200446522016	37.0	37.0	37.0	33.0	37.0
39	35.15587664829415	37.0	37.0	37.0	33.0	37.0
40	34.927113653805904	37.0	37.0	37.0	27.0	37.0
41	35.09946975511058	37.0	37.0	37.0	27.0	37.0
42	35.105433614735226	37.0	37.0	37.0	33.0	37.0
43	34.741647945301054	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	23.0
16	39.0
17	35.0
18	27.0
19	29.0
20	43.0
21	143.0
22	356.0
23	809.0
24	1634.0
25	2930.0
26	4966.0
27	7498.0
28	10523.0
29	14208.0
30	18469.0
31	23646.0
32	30455.0
33	39947.0
34	56456.0
35	112059.0
36	392348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58787413660783	20.363636363636363	13.576641317239934	23.471848182515874
2	17.07095513849159	21.82041442824252	38.76062234005442	22.34800809321147
3	17.44952208190888	29.250122095862697	29.699434870578383	23.60092095165004
4	11.400125584315916	18.615921300495362	41.708086234563595	28.275866880625134
5	13.082397265052675	37.53519849298821	35.60706062931696	13.775343612642155
6	26.680108839740456	42.505965255005925	17.096490616060837	13.717435289192773
7	26.29819298123212	32.5154538477639	23.508546710388615	17.677806460615365
8	24.23037745063839	34.542384706621085	20.15251517477151	21.074722667969024
9	27.225144770808622	14.220609781622828	19.89395102211679	38.66029442545175
10	16.632805414079396	26.441498639503248	33.01751203516361	23.90818391125375
11	33.773529616967835	24.665596874345916	23.31347240633503	18.24740110235122
12	23.796134793832415	27.313332868206235	27.923393567292266	20.967138770669084
13	28.72057489709063	22.919835345008025	25.201841903300075	23.157747854601272
14	22.91257936231075	21.370264424754065	27.96623177283193	27.750924440103258
15	25.546780157678086	27.49668596944115	25.9159980464662	21.040535826414565
16	22.058885090350937	27.767110863043325	25.76864578246006	24.405358264145676
17	22.499546501081422	26.385125235470593	25.40235819437661	25.71297006907137
18	23.419102769831856	25.851112816577132	28.16172469127189	22.568059722319123
19	26.617735296169677	24.57015279425103	28.220330705365242	20.59178120421405
20	25.62352612851462	24.38442754482662	28.45112677039001	21.54091955626875
21	22.960301402358194	26.90239307890881	28.163120072559828	21.974185446173166
22	22.74764529407661	26.089164864299168	28.170236517128306	22.992953324495918
23	22.668248098792994	26.430754203586133	29.556687364822437	21.34431033279844
24	22.764529407660643	27.467662038652062	26.322193539384635	23.44561501430266
25	23.060489778832068	26.734389171841205	29.1172817972511	21.08783925207563
26	22.995185934556616	27.04318705086165	28.300983743807993	21.66064327077374
27	23.068722528430893	26.768576013395663	27.384357775762226	22.77834368241122
28	21.87329937905533	26.35261285146166	29.32282146096421	22.451266308518804
29	24.921091188167168	26.04911742133538	27.0420707458313	21.987720644666155
30	23.83562408428103	25.516221307472264	28.724481964696853	21.92367264354985
31	23.558780436754343	25.679480918160884	28.352194237075278	22.409544408009488
32	21.312495639433475	25.666085257796695	28.960720016744574	24.060699086025256
33	21.50910486290379	25.9270215586409	29.679062303774508	22.884811274680807
34	22.79453010535129	25.572176097118536	28.97272029582083	22.66057350170934
35	22.625549431382126	26.819786506662947	27.918649270913278	22.636014791041653
36	23.9867438777646	26.4447080164655	27.24356380380939	22.32498430196051
37	23.147422033070537	25.411149096490615	27.887950882578664	23.553477987860184
38	23.12900300006977	26.388613688690434	27.29003000069769	23.192353310542106
39	22.335310123491244	26.16200376752948	28.30070466755041	23.20198144142887
40	22.587734598479035	27.263378218098094	28.4579641387009	21.69092304472197
41	22.98806948998814	25.375008721133053	28.040186981092585	23.596734807786227
42	21.519151608176934	27.85557803669853	27.748273215656177	22.87699713946836
43	23.345984790343962	25.600641875392448	27.77520407451336	23.278169259750225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	50.0
2	65.0
3	269.0
4	473.0
5	473.0
6	657.0
7	841.0
8	795.5
9	750.0
10	1080.0
11	1410.0
12	1410.0
13	2136.5
14	2863.0
15	5149.5
16	7436.0
17	8029.0
18	8622.0
19	8622.0
20	6562.5
21	4503.0
22	3224.0
23	1945.0
24	2261.0
25	2577.0
26	2577.0
27	3937.0
28	5297.0
29	7598.5
30	9900.0
31	13336.5
32	16773.0
33	16773.0
34	25540.0
35	34307.0
36	37749.0
37	41191.0
38	46495.5
39	51800.0
40	51800.0
41	57609.0
42	63418.0
43	66607.0
44	69796.0
45	69341.5
46	68887.0
47	68887.0
48	67399.0
49	65911.0
50	65600.5
51	65290.0
52	61114.0
53	56938.0
54	56938.0
55	53700.0
56	50462.0
57	39929.5
58	29397.0
59	26389.5
60	23382.0
61	23382.0
62	19742.0
63	16102.0
64	11431.0
65	6760.0
66	5641.5
67	4523.0
68	4523.0
69	3554.0
70	2585.0
71	1912.5
72	1240.0
73	1064.5
74	889.0
75	889.0
76	528.0
77	167.0
78	111.0
79	55.0
80	43.5
81	32.0
82	32.0
83	23.5
84	15.0
85	8.5
86	2.0
87	4.0
88	6.0
89	6.0
90	4.5
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	716650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.27091322118699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56451017315673	13.440907815328742
2	9.209164830054151	3.3651970289905324
3	3.614903545920932	1.9814276697144746
4	2.1124631623795054	1.5438652449116073
5	1.422554738307769	1.2995687088004808
6	1.0055084092578355	1.102293413323421
7	0.818862886725397	1.047296092038728
8	0.6510034803216684	0.9515542476518334
9	0.5937965809224609	0.976428522096464
>10	5.261713820111276	20.79387264483979
>50	0.8864794181727165	11.332459807360925
>100	0.7729063886733746	27.58314477936019
>500	0.06478998327143673	8.13432522769917
>1k	0.02058034762739755	5.569299853713358
>5k	7.622350973110202E-4	0.8783589441702964
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6307	0.880066978301821	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4417	0.6163399148817414	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2391	0.3336356659457197	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1898	0.2648433684504291	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1779	0.24823833112397964	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1736	0.24223819158585083	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1711	0.23874973836600852	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1537	0.21447010395590593	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1501	0.20944673131933303	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1496	0.20874904067536454	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1362	0.19005093141700968	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1355	0.18907416451545386	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1343	0.18739970696992952	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1323	0.18460894439405567	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1318	0.1839112537500872	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1280	0.17860880485592687	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1232	0.17191097467382963	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1216	0.16967836461313054	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1202	0.16772483081001885	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1200	0.16744575455243146	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1173	0.16367822507500174	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1167	0.16284099630223958	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1155	0.16116653875671527	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1070	0.14930579780925138	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	1051	0.14665457336217122	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1044	0.14567780646061537	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	1032	0.14400334891509103	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1001	0.13967766692248657	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	999	0.13939859066489918	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	977	0.13632875183143794	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	977	0.13632875183143794	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	974	0.13591013744505687	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	962	0.13423567989953256	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	950	0.13256122235400825	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	941	0.13130537919486499	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	941	0.13130537919486499	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	941	0.13130537919486499	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	937	0.13074722667969024	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	934	0.13032861229330917	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	910	0.12697969720226052	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	871	0.1215377101793065	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	868	0.12111909579292542	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	859	0.11986325263378218	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	850	0.11860740947463894	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	819	0.11428172748203447	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	801	0.11177004116374799	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	795	0.11093281239098585	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	789	0.11009558361822368	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	783	0.10925835484546154	No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC	774	0.10800251168631829	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	758	0.1057699016256192	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	755	0.10535128723923812	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	742	0.10353729156492011	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	731	0.1020023721481895	No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT	730	0.1018628340193958	No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC	727	0.10144421963301471	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	725	0.10116514337542733	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	718	0.1001883764738715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.581525151747715E-4	0.0	0.0
2	2.7907625758738573E-4	0.0	8.372287727621573E-4	1.3953812879369286E-4	0.0
3	2.7907625758738573E-4	0.0	9.767669015558502E-4	1.3953812879369286E-4	0.0
4	2.7907625758738573E-4	0.0	0.001116305030349543	4.1861438638107865E-4	0.0
5	2.7907625758738573E-4	0.0	0.0015349194167306216	5.581525151747715E-4	0.0
6	2.7907625758738573E-4	0.0	0.0032093769622549362	5.581525151747715E-4	0.0
7	2.7907625758738573E-4	0.0	0.008511825856415266	6.976906439684644E-4	0.0
8	2.7907625758738573E-4	0.0	0.009628130886764808	6.976906439684644E-4	0.0
9	4.1861438638107865E-4	0.0	0.011581664689876509	0.001255843159143236	0.0
10	9.767669015558502E-4	0.0	0.01353519849298821	0.001255843159143236	0.0
11	9.767669015558502E-4	0.0	0.019256261773529616	0.0013953812879369289	0.0
12	9.767669015558502E-4	0.0	0.021767948091816088	0.0016744575455243146	0.0
13	9.767669015558502E-4	0.0	0.024558710667689946	0.0016744575455243146	0.0
14	9.767669015558502E-4	0.0	0.025535477569245797	0.0016744575455243146	0.0
15	9.767669015558502E-4	0.0	0.027489011372357496	0.0019535338031117003	0.0
16	9.767669015558502E-4	0.0	0.029861159561850276	0.0019535338031117003	0.0
17	9.767669015558502E-4	0.0	0.030419312077025046	0.0020930719319053933	0.0
18	9.767669015558502E-4	0.0	0.0313960789785809	0.002511686318286472	0.0
19	9.767669015558502E-4	0.0	0.03209376962254936	0.002511686318286472	0.0
20	9.767669015558502E-4	0.0	0.03279146026651782	0.0027907625758738577	0.0
21	9.767669015558502E-4	0.0	0.03432637968324845	0.0034884532198423217	0.0
22	9.767669015558502E-4	0.0	0.03488453219842322	0.004325681992604479	0.0
23	9.767669015558502E-4	0.0	0.03502407032721691	0.0058606014093351005	0.0
24	9.767669015558502E-4	0.0	0.035303146584804296	0.006279215795716179	0.0
25	9.767669015558502E-4	0.0	0.03572176097118537	0.007255982697272029	0.0
26	9.767669015558502E-4	0.0	0.03572176097118537	0.007814135212446801	0.0
27	0.001116305030349543	0.0	0.03572176097118537	0.01018628340193958	0.0
28	0.001116305030349543	0.0	0.03572176097118537	0.026093630084420567	0.0
29	0.001116305030349543	0.0	0.03572176097118537	0.06139677666922486	0.0
30	0.001116305030349543	0.0	0.03572176097118537	0.10423498220888858	0.0
31	0.001116305030349543	0.0	0.03572176097118537	0.20023721481894927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAATC	25	0.0054949806	29.599998	2
CCTGTAG	25	0.0054949806	29.599998	10
CTTGGGA	25	0.0054949806	29.599998	3
TTGACAA	40	5.9370563E-5	27.750002	34
GGTATCA	1010	0.0	27.292078	1
CGCTCTC	160	0.0	26.593752	29
AACAAGA	35	8.8661246E-4	26.42857	13
CCCAACG	35	8.8661246E-4	26.42857	21
CCGCTCT	170	0.0	25.029411	28
GCTCTCT	170	0.0	25.029411	30
GACAATC	45	1.3225328E-4	24.666668	36
CTAGTGC	45	1.3225328E-4	24.666668	9
ATATACA	75	1.3718818E-8	24.666666	1
GTATCAA	3135	0.0	24.194578	1
GCCGCTC	170	0.0	23.941175	27
CGTTATT	70	1.9208164E-7	23.785713	10
TATACAA	70	1.9208164E-7	23.785713	2
TTATTGT	70	1.9208164E-7	23.785713	12
CCGTCAA	55	1.9013632E-5	23.545454	24
CCCCGTC	55	1.9013632E-5	23.545454	22
>>END_MODULE