FastQCFastQC Report
Fri 10 Feb 2017
ERR1633179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605064
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43790.7237250935438234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30970.511846680681713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18360.30343897505057316No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10830.17898933005434137No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8430.1393241045575344No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA8180.13519231023495035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.12858143931881585No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7670.12676344981687887No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7540.12461491676913516No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6990.11552496925945024No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6910.11420279507622334No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6840.1130458926658998No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6240.10312958629169808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCG250.00549431129.65
CGCGAAG250.00549431129.610
GGTATCA7900.025.5253161
GTATCAA22000.022.9568181
CCGCTCT1300.022.7692328
TTAACGG603.7225327E-521.58333235
CGCTCTC1251.8189894E-1120.7229
CTAACCG450.003823745520.5555556
GCCTAGT450.003823745520.5555551
CGCACGG450.003823745520.55555525
GCCGCTC1303.274181E-1119.92307727
TGCTCGC1355.638867E-1119.18518610
CGGGTCC500.00703112518.517
GCTATAC500.00703112518.53
AACGGCC701.2179863E-418.537
TAACCGT500.00703112518.57
TTCGCCG1409.276846E-1118.524
CGTGGGA1850.018.037
GCCGGCA1451.546141E-1017.86206815
CATGCTA1258.554707E-917.764
Produced by FastQC (version 0.11.5)