##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633179.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	605064
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71728279983605	33.0	33.0	33.0	27.0	33.0
2	31.865753044306057	33.0	33.0	33.0	33.0	33.0
3	31.586511509526265	33.0	33.0	33.0	27.0	33.0
4	31.883597437626435	33.0	33.0	33.0	33.0	33.0
5	31.994212182512925	33.0	33.0	33.0	33.0	33.0
6	35.115675366572795	37.0	37.0	37.0	33.0	37.0
7	35.2679485145373	37.0	37.0	37.0	33.0	37.0
8	35.39699436753798	37.0	37.0	37.0	33.0	37.0
9	35.51922275990639	37.0	37.0	37.0	33.0	37.0
10	35.544023442148266	37.0	37.0	37.0	33.0	37.0
11	35.55863346687293	37.0	37.0	37.0	33.0	37.0
12	35.551273914825536	37.0	37.0	37.0	33.0	37.0
13	35.53597966496106	37.0	37.0	37.0	33.0	37.0
14	35.51156902410324	37.0	37.0	37.0	33.0	37.0
15	35.53861079158568	37.0	37.0	37.0	33.0	37.0
16	35.51094429680166	37.0	37.0	37.0	33.0	37.0
17	35.51016256131583	37.0	37.0	37.0	33.0	37.0
18	35.49300239313527	37.0	37.0	37.0	33.0	37.0
19	35.47642563431307	37.0	37.0	37.0	33.0	37.0
20	35.493389129083866	37.0	37.0	37.0	33.0	37.0
21	35.477283394814435	37.0	37.0	37.0	33.0	37.0
22	35.384721285682176	37.0	37.0	37.0	33.0	37.0
23	35.430551148308275	37.0	37.0	37.0	33.0	37.0
24	35.40335567807703	37.0	37.0	37.0	33.0	37.0
25	35.42211732973702	37.0	37.0	37.0	33.0	37.0
26	35.305967633175996	37.0	37.0	37.0	33.0	37.0
27	35.325363928443934	37.0	37.0	37.0	33.0	37.0
28	35.346026205492315	37.0	37.0	37.0	33.0	37.0
29	35.35933884679968	37.0	37.0	37.0	33.0	37.0
30	35.35479552576256	37.0	37.0	37.0	33.0	37.0
31	35.333260613753254	37.0	37.0	37.0	33.0	37.0
32	35.32472928483598	37.0	37.0	37.0	33.0	37.0
33	35.309613528486246	37.0	37.0	37.0	33.0	37.0
34	35.29453413192654	37.0	37.0	37.0	33.0	37.0
35	35.222288881837294	37.0	37.0	37.0	33.0	37.0
36	35.24716228365925	37.0	37.0	37.0	33.0	37.0
37	35.24292140996655	37.0	37.0	37.0	33.0	37.0
38	35.217208427538246	37.0	37.0	37.0	33.0	37.0
39	35.11637116073671	37.0	37.0	37.0	33.0	37.0
40	34.8968076104346	37.0	37.0	37.0	27.0	37.0
41	35.05426698663282	37.0	37.0	37.0	27.0	37.0
42	35.091641545357184	37.0	37.0	37.0	33.0	37.0
43	34.7245944230693	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	27.0
16	40.0
17	30.0
18	31.0
19	23.0
20	47.0
21	115.0
22	327.0
23	783.0
24	1603.0
25	2812.0
26	4606.0
27	6677.0
28	9489.0
29	12390.0
30	16329.0
31	20387.0
32	25978.0
33	34011.0
34	48441.0
35	96835.0
36	324078.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.973906892494014	20.554354580672456	14.224115134927876	23.247623391905652
2	15.751722131873652	22.752634432060077	39.7807504660664	21.71489296999987
3	18.1035394602885	27.958860550288893	31.347097166580724	22.59050282284188
4	12.317705234487592	18.292445096717042	40.14550526886412	29.24434439993125
5	13.452461227242077	37.666098131767875	34.93597371517724	13.945466925812807
6	27.75227083415969	40.869726177726655	17.587726257057103	13.79027673105655
7	24.982977007390954	33.708004442505256	23.22299789774305	18.086020652360745
8	24.0022543069824	35.75572170877791	20.96720346938506	19.274820514854625
9	25.998076236563406	15.431425435986936	20.27967289410707	38.29082543334259
10	14.704890722303757	28.368899818862136	33.74254624304206	23.18366321579205
11	33.005268864120154	24.646153134213904	24.22190049317097	18.12667750849497
12	22.52389829836183	26.393406317348244	30.683530998373726	20.3991643859162
13	28.27238110348657	22.955918712731215	26.772705036161465	21.998995147620747
14	22.012712704771726	22.080639403435008	28.794639905861196	27.112007985932067
15	23.927716737402985	28.59152089696297	26.044021789430538	21.43674057620351
16	21.679855354144355	29.200712651884757	25.733145584599313	23.38628640937157
17	21.921317414356167	27.69211190882287	26.636521095289094	23.750049581531872
18	22.650496476405802	26.495379001229622	28.749190168312772	22.104934354051803
19	24.917859928867028	26.28845874155461	28.841081274047042	19.952600055531317
20	24.94198960771092	25.315503814472518	28.81298507265347	20.92952150516309
21	22.576454722145094	27.44883185910912	28.120496344188382	21.854217074557404
22	21.990235743656868	27.305706503774807	28.25750664392527	22.446551108643053
23	22.212195734666086	26.99119431993971	28.90173601470258	21.89487393069163
24	22.902866473629235	26.977476762788733	28.07471606309415	22.044940700487885
25	22.747511007100073	26.78361297317309	28.85083230864834	21.618043711078496
26	22.748833181283302	26.811048087475044	28.222303756296856	22.2178149749448
27	22.63215130961353	26.883271852233815	28.3917073235228	22.092869514629857
28	21.808271521690266	27.338595586582574	28.5146695225629	22.338463369164256
29	22.89063636243439	27.24488649134637	27.76053442280486	22.103942723414384
30	22.376475876932027	27.275627041106393	28.69993917998757	21.647957901974006
31	22.926004521835708	26.692052410984623	28.313699046712415	22.068244020467258
32	21.299399732920815	26.899964301297054	28.831826054764452	22.968809911017676
33	21.774721351790884	26.79154601827245	29.29557864953129	22.138153980405377
34	21.54648103341134	27.05052688641202	28.502935226686766	22.90005685348988
35	22.131708381262147	27.6101371094628	28.177019290521333	22.08113521875372
36	22.463408829479196	27.11812304152949	28.291883172689168	22.126584956302143
37	23.13755239115201	26.529259714674815	27.885645154892707	22.447542739280475
38	22.932450120978938	27.188363545013424	27.77673105654939	22.10245527745825
39	22.448038554599183	26.630240767918767	28.421952057964116	22.499768619517933
40	22.54769743365991	27.144235981648222	28.282627953406582	22.025438631285287
41	22.404737350098504	26.711389208414317	28.540782462681634	22.343090978805545
42	22.207898668570596	27.068376237885577	28.392368410614417	22.33135668292941
43	22.053369560905953	26.938637896156443	28.211395819285233	22.796596723652375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	50.0
2	69.0
3	235.0
4	401.0
5	401.0
6	490.5
7	580.0
8	580.0
9	580.0
10	803.5
11	1027.0
12	1027.0
13	1590.5
14	2154.0
15	4128.5
16	6103.0
17	6981.5
18	7860.0
19	7860.0
20	7306.0
21	6752.0
22	7338.5
23	7925.0
24	9569.5
25	11214.0
26	11214.0
27	13167.5
28	15121.0
29	18940.0
30	22759.0
31	24182.0
32	25605.0
33	25605.0
34	28292.5
35	30980.0
36	32013.0
37	33046.0
38	35648.5
39	38251.0
40	38251.0
41	40793.0
42	43335.0
43	42050.0
44	40765.0
45	42092.5
46	43420.0
47	43420.0
48	44086.5
49	44753.0
50	45447.0
51	46141.0
52	46749.5
53	47358.0
54	47358.0
55	41908.5
56	36459.0
57	32585.0
58	28711.0
59	25276.0
60	21841.0
61	21841.0
62	18786.0
63	15731.0
64	12463.0
65	9195.0
66	7709.0
67	6223.0
68	6223.0
69	5114.0
70	4005.0
71	3466.0
72	2927.0
73	2328.0
74	1729.0
75	1729.0
76	1257.5
77	786.0
78	617.0
79	448.0
80	341.0
81	234.0
82	234.0
83	215.5
84	197.0
85	179.5
86	162.0
87	128.5
88	95.0
89	95.0
90	79.5
91	64.0
92	43.0
93	22.0
94	13.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	605064.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.79477881081385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.43475915792028	48.45763067245837
2	6.622205463467251	7.257245672209021
3	1.5759496162682394	2.590614319212154
4	0.7123483609286044	1.5613188349331468
5	0.44747563943730445	1.2259664343097294
6	0.2896635323863401	0.9523229512001112
7	0.2077370391416867	0.7968033577407472
8	0.16645841312244192	0.7296841542594623
9	0.12434753068142192	0.6132235895423462
>10	1.108483822860027	12.846345103932425
>50	0.17935269912415225	6.872090307056738
>100	0.12478042275121662	12.48843265819838
>500	0.005211958689854249	1.8620345657524413
>1k	0.0012263432211421764	1.7462873791949323
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4379	0.7237250935438234	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3097	0.511846680681713	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1836	0.30343897505057316	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1083	0.17898933005434137	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	843	0.1393241045575344	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	818	0.13519231023495035	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	778	0.12858143931881585	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	767	0.12676344981687887	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	754	0.12461491676913516	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	699	0.11552496925945024	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	691	0.11420279507622334	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	684	0.1130458926658998	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	624	0.10312958629169808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.3054354580672456E-4	0.0	0.0
2	0.0	0.0	3.3054354580672456E-4	0.0	0.0
3	0.0	0.0	6.610870916134491E-4	0.0	0.0
4	0.0	0.0	8.263588645168115E-4	3.3054354580672456E-4	0.0
5	0.0	0.0	0.001156902410323536	3.3054354580672456E-4	0.0
6	0.0	0.0	0.002313804820647072	4.958153187100869E-4	0.0
7	0.0	0.0	0.004131794322584057	9.916306374201738E-4	0.0
8	0.0	0.0	0.004792881414197507	9.916306374201738E-4	0.0
9	1.6527177290336228E-4	0.0	0.005619240278714317	0.001156902410323536	0.0
10	6.610870916134491E-4	0.0	0.006280327370327767	0.0013221741832268982	0.0
11	6.610870916134491E-4	0.0	0.009585762828395013	0.0014874459561302607	0.0
12	6.610870916134491E-4	0.0	0.010246849920008462	0.0014874459561302607	0.0
13	6.610870916134491E-4	0.0	0.010412121692911825	0.0014874459561302607	0.0
14	6.610870916134491E-4	0.0	0.010577393465815186	0.0014874459561302607	0.0
15	6.610870916134491E-4	0.0	0.011734295876138723	0.0014874459561302607	0.0
16	6.610870916134491E-4	0.0	0.013221741832268983	0.001652717729033623	0.0
17	6.610870916134491E-4	0.0	0.013221741832268983	0.0019832612748403476	0.0
18	6.610870916134491E-4	0.0	0.013221741832268983	0.00214853304774371	0.0
19	6.610870916134491E-4	0.0	0.013387013605172346	0.00214853304774371	0.0
20	6.610870916134491E-4	0.0	0.013882828923882432	0.0028096201393571587	0.0
21	6.610870916134491E-4	0.0	0.014213372469689158	0.0036359790038739703	0.0
22	6.610870916134491E-4	0.0	0.014378644242592518	0.004131794322584057	0.0
23	6.610870916134491E-4	0.0	0.014378644242592518	0.005123424960004231	0.0
24	6.610870916134491E-4	0.0	0.014378644242592518	0.0061150555974244045	0.0
25	6.610870916134491E-4	0.0	0.014543916015495881	0.0067761426890378534	0.0
26	6.610870916134491E-4	0.0	0.014543916015495881	0.007437229780651303	0.0
27	6.610870916134491E-4	0.0	0.014543916015495881	0.009585762828395013	0.0
28	6.610870916134491E-4	0.0	0.014543916015495881	0.018510438565176576	0.0
29	6.610870916134491E-4	0.0	0.014543916015495881	0.03652506181164306	0.0
30	6.610870916134491E-4	0.0	0.014543916015495881	0.0626380019303743	0.0
31	6.610870916134491E-4	0.0	0.014543916015495881	0.12263165549429482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACCG	25	0.005494311	29.6	5
CGCGAAG	25	0.005494311	29.6	10
GGTATCA	790	0.0	25.525316	1
GTATCAA	2200	0.0	22.956818	1
CCGCTCT	130	0.0	22.76923	28
TTAACGG	60	3.7225327E-5	21.583332	35
CGCTCTC	125	1.8189894E-11	20.72	29
CTAACCG	45	0.0038237455	20.555555	6
GCCTAGT	45	0.0038237455	20.555555	1
CGCACGG	45	0.0038237455	20.555555	25
GCCGCTC	130	3.274181E-11	19.923077	27
TGCTCGC	135	5.638867E-11	19.185186	10
CGGGTCC	50	0.007031125	18.5	17
GCTATAC	50	0.007031125	18.5	3
AACGGCC	70	1.2179863E-4	18.5	37
TAACCGT	50	0.007031125	18.5	7
TTCGCCG	140	9.276846E-11	18.5	24
CGTGGGA	185	0.0	18.0	37
GCCGGCA	145	1.546141E-10	17.862068	15
CATGCTA	125	8.554707E-9	17.76	4
>>END_MODULE