FastQCFastQC Report
Fri 10 Feb 2017
ERR1633178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538424
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT108832.0212694827868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84791.5747812133188714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46000.8543452743562694No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA35600.6611889514583302No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT32520.6039849635231713No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30150.559967609170468No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG28340.5263509798968842No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC26500.4921771689226335No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG26090.4845623523468493No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23420.4349731809874745No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20940.38891282706565833No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA20040.37219737604564435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.3710830126443101No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG19240.35733919736118747No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC17430.3237225680876038No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17380.3227939319198253No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC17260.32056520511715675No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC16850.3129503885413726No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC15890.29512057412002435No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15310.28434839457379313No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA15130.2810053043697904No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14140.262618308247775No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA13870.2576036729417708No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC13640.2533319465699895No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC13590.25240331040221087No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13170.24460276659287106No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT13160.2444170393593153No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13060.24255976702375823No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12840.23847376788553257No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA12810.23791658618486544No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12690.2356878593821969No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12580.23364485981308408No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC12150.22565858877018857No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11940.2217583168655186No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT11880.22064395346418436No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC11860.2202724989970729No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11770.21860095389507153No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT11750.21822949942796013No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC11650.216372227092403No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT11530.21414350028973447No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC11490.21340059135551165No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA11320.21024322838506457No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG11240.20875741051661886No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC10810.2007711394737233No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10780.20021395777305617No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA10740.19947104883883335No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.19928532160527762No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA10660.19798523097038764No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT10460.19427068629927344No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10400.19315632289793916No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10310.19148477779593778No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10230.18999895992749208No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10200.18944177822682495No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9950.1847985973879322No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA9810.18219841611815224No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG9700.1801554165490394No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG9510.1766265991114809No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9460.17569796294370235No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9430.17514078124303523No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9290.17254059997325527No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9030.1677116919008068No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG9000.16715451020013966No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8950.1662258740323611No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8940.1660401467988054No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG8760.1626970565948026No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG8740.16232560212769118No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8730.16213987489413548No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8640.16046832979213407No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8600.15972542085791122No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG8420.15638233065390844No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8260.1534106949170171No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG8210.1524820587492385No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8130.15099624088079283No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC7950.14765315067679002No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7920.1470959689761229No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7740.14375287877212012No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7730.1435671515385644No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7560.1404097885681173No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG7550.1402240613345616No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7520.1396668796338945No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC7500.13929542516678306No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC7380.13706669836411453No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7370.1368809711305588No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC7370.1368809711305588No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA7340.13632378942989168No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7330.13613806219633595No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG7270.1350236987950017No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7240.13446651709433458No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA7110.13205206305811035No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7070.1313091541238875No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT6980.1296376090218861No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6970.12945188178833036No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6710.1246229737158819No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6690.12425151924877048No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6630.12313715584743622No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA6550.12165133797899053No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6430.11942261117632201No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6410.1190511567092106No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG6390.11867970224209916No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC6390.11867970224209916No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA6380.11849397500854346No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA6300.11700815714009777No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6240.1158937937387635No Hit
GTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCG6240.1158937937387635No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT6080.11292215800187214No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA6060.1125507035347607No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG6030.11199352183409357No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5880.10920761333075792No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5880.10920761333075792No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC5850.10865043163009079No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5810.10790752269586794No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.10716461376164509No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG5680.1054930686596437No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA5660.10512161419253228No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA5590.1038215235576423No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5500.10214997845564092No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5420.10066416058719521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA200.001841067137.010
TTGCTCT352.3859671E-531.71428716
TGCTCTC352.3859671E-531.71428717
ATGGGCA303.5969596E-430.83333430
GACCCGA303.5969596E-430.8333346
TAGCAAC303.5969596E-430.8333341
GCTCTCG303.5969596E-430.83333418
TAACAAC303.5969596E-430.8333341
TGGGCAG303.5969596E-430.83333431
TGATCTA303.5969596E-430.83333420
GGTATCA17850.029.6414591
ACAACGC358.8632456E-426.4285723
AGCAACG358.8632456E-426.4285722
GGCAGGT358.8632456E-426.42857233
GCGGTTC358.8632456E-426.42857228
CGAAACC358.8632456E-426.42857210
CGACGCA358.8632456E-426.42857216
TACGACG358.8632456E-426.42857214
TCTCGCG358.8632456E-426.42857220
AAACCCG358.8632456E-426.42857212
Produced by FastQC (version 0.11.5)