FastQCFastQC Report
Fri 10 Feb 2017
ERR1633177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695449
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188332.708034665374456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142122.043571850703646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73471.05643979644805No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA39230.5640960012883763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35430.5094550427134125No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG31200.4486310282997028No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT30210.4343956206709622No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30080.4325263247197135No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG29250.4205915890309713No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC25110.3610617025835108No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23790.3420811590785234No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG21320.3065645360047969No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA20660.2970742642523032No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.26515244108482433No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18310.26328314513357554No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC17400.25019807347483425No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17220.247609817542336No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA17160.24674706556483653No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC16640.23926988175984149No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16640.23926988175984149No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC16290.23423716189109484No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15910.22877306603359843No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15090.21698212234110623No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC14740.2119494024723596No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14610.2100801065211108No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14310.20576634663361368No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC14160.2036094666898651No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14070.20231533872361596No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13940.20044604277236722No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13730.1974264108511192No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13650.19627607488111998No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA13270.1908119790236236No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12820.18434133919237788No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12770.18362237921112837No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA12770.18362237921112837No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT12420.17858965934238166No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT12260.17628898740238322No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12000.17255039549988568No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11830.17010593156363732No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC11760.16909938758988796No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT11640.16737388363488911No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11550.16607975566863997No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11490.16521700369114053No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11440.16449804370989102No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11100.15960911583739426No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11090.15946532384114434No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10970.1577398198861455No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.15673327591239616No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10800.15529535594989713No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10770.15486397996114742No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10760.1547201879648975No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10620.15270710001739882No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10570.15198814003614933No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC10180.14638025218240303No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10150.14594887619365332No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.1456612922011535No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9960.14321682826490512No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA9940.1429292442724053No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9920.1426416602799055No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9920.1426416602799055No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG9800.14091615632490664No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9780.14062857233240683No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA9760.14034098833990702No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG9630.13847169238865828No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9610.13818410839615847No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9520.1368899804299093No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9460.1360272284524099No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA9240.13286380453491198No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9190.13214484455366246No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9130.13128209257616302No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG9040.12998796460991388No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA8990.12926900462866436No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8980.12912521263241447No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG8980.12912521263241447No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8890.1278310846661653No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8840.12711212468491578No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8740.12567420472241675No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8730.12553041272616683No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG8580.12337353278241826No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG8570.12322974078616837No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8410.12092906884616988No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8370.12035390086117027No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8350.12006631686867046No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8000.1150335969999238No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7930.11402705302617445No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG7830.1125891330636754No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7770.11172638108617597No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7600.1092819171499276No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG7550.10856295716867809No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC7500.10784399718742856No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC7470.10741262119867885No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7460.10726882920242893No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7370.1059747012361798No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7350.10568711724367999No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA7310.10511194925868036No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC7300.10496815726243046No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG7270.10453678127368075No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7220.10381782129243121No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC7220.10381782129243121No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA7170.1030988613111817No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7140.10266748532243197No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT7130.10252369332618208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAATA200.001841436537.010
TCCAAAA200.001841436537.013
CATTTTG401.5988644E-632.37536
CCATTTT401.5988644E-632.37535
CACAGAG303.5980294E-430.8333322
CCTAGTT551.8535502E-830.2727282
CTCTCGC551.8535502E-830.27272819
TAGACCG250.0054948729.5999985
TTGCTCT502.7181704E-729.59999816
ACTTCTA250.0054948729.59999824
ACAGAGT250.0054948729.5999983
AGCAACG454.0052655E-628.7777772
CTTCCCT454.0052655E-628.77777735
TTCCCTG405.936809E-527.7536
TAGTTCC604.317735E-827.7499984
CTAGTTC604.317735E-827.7499983
TCTCGCG556.2557774E-726.9090920
GCCTAGT706.5829227E-926.4285721
ACCATTT509.084348E-625.89999834
TAGCAAC509.084348E-625.8999981
Produced by FastQC (version 0.11.5)