FastQCFastQC Report
Fri 10 Feb 2017
ERR1633176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657566
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29410.4472554846205552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.32863621294288325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11230.17078133601798146No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG10960.16667528430606204No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA10120.15390090120231278No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8680.13200195873874257No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7980.12135663948561817No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG7880.11983587959231469No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7840.1192275756349933No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC7050.10721357247789577No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG7030.10690942049923506No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6760.10280336878731565No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6620.10067430493669077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC405.936321E-527.7500023
CCTTCCG358.865344E-426.428578
TGCGGTA601.3359531E-624.66666836
GTATTGC400.001930312523.1250021
CGAGTCG802.72139E-823.12500221
ATTTGCG400.001930312523.12500216
GGTATCA6700.022.641791
GTATCAA17850.022.5938381
TTGCGCG450.003824093820.55555518
GTCAACC1601.8189894E-1218.515
TTAACGG2100.018.535
TAGCACC500.007031759718.4999984
CCGCGCG500.007031759718.49999830
GTACATG3050.018.1967221
CATGCTA2450.018.122454
GCGAACT2500.017.7628
TAACGGC2200.017.6590936
GCACCGC2200.017.6590910
AGTCGCC953.6016409E-617.52631623
GACGTGC852.720248E-517.41176432
Produced by FastQC (version 0.11.5)