##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633176.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657566
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43273831067908	33.0	33.0	33.0	27.0	33.0
2	31.648585845375216	33.0	33.0	33.0	27.0	33.0
3	31.37953452581186	33.0	33.0	33.0	27.0	33.0
4	31.69386342967854	33.0	33.0	33.0	27.0	33.0
5	31.808919560926203	33.0	33.0	33.0	33.0	33.0
6	34.775902342882695	37.0	37.0	37.0	27.0	37.0
7	34.943944790332836	37.0	37.0	37.0	27.0	37.0
8	35.067451784307586	37.0	37.0	37.0	33.0	37.0
9	35.213806674919326	37.0	37.0	37.0	33.0	37.0
10	35.24188902710907	37.0	37.0	37.0	33.0	37.0
11	35.223139578384526	37.0	37.0	37.0	33.0	37.0
12	35.23248312716898	37.0	37.0	37.0	33.0	37.0
13	35.22821587490837	37.0	37.0	37.0	33.0	37.0
14	35.215766934421794	37.0	37.0	37.0	33.0	37.0
15	35.22036875385893	37.0	37.0	37.0	33.0	37.0
16	35.19846829063547	37.0	37.0	37.0	33.0	37.0
17	35.19976701958434	37.0	37.0	37.0	33.0	37.0
18	35.18717816918758	37.0	37.0	37.0	33.0	37.0
19	35.177171569089644	37.0	37.0	37.0	33.0	37.0
20	35.17983137814303	37.0	37.0	37.0	33.0	37.0
21	35.16983238184456	37.0	37.0	37.0	33.0	37.0
22	35.07468451836014	37.0	37.0	37.0	33.0	37.0
23	35.1019654300861	37.0	37.0	37.0	33.0	37.0
24	35.104956764796235	37.0	37.0	37.0	33.0	37.0
25	35.12141138684178	37.0	37.0	37.0	33.0	37.0
26	35.00802504995696	37.0	37.0	37.0	27.0	37.0
27	35.01515437233677	37.0	37.0	37.0	27.0	37.0
28	35.04205661484931	37.0	37.0	37.0	27.0	37.0
29	35.027407134797116	37.0	37.0	37.0	27.0	37.0
30	35.03034372215109	37.0	37.0	37.0	27.0	37.0
31	35.03050036042009	37.0	37.0	37.0	27.0	37.0
32	35.02652965633868	37.0	37.0	37.0	27.0	37.0
33	35.021795530790826	37.0	37.0	37.0	27.0	37.0
34	35.00154205053181	37.0	37.0	37.0	27.0	37.0
35	34.911960168256876	37.0	37.0	37.0	27.0	37.0
36	34.95530030445613	37.0	37.0	37.0	27.0	37.0
37	34.948668270561434	37.0	37.0	37.0	27.0	37.0
38	34.9452708929598	37.0	37.0	37.0	27.0	37.0
39	34.83825654002792	37.0	37.0	37.0	27.0	37.0
40	34.62102663458877	37.0	37.0	37.0	27.0	37.0
41	34.815335646916054	37.0	37.0	37.0	27.0	37.0
42	34.846345461900405	37.0	37.0	37.0	27.0	37.0
43	34.47271452599435	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	27.0
16	39.0
17	39.0
18	36.0
19	41.0
20	88.0
21	231.0
22	581.0
23	1222.0
24	2346.0
25	4011.0
26	6204.0
27	9040.0
28	12371.0
29	16052.0
30	20650.0
31	25825.0
32	32221.0
33	42405.0
34	61399.0
35	117398.0
36	305337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92461289056916	20.85828646858262	13.334631048442287	23.882469592405933
2	16.697183248525622	22.233813792075622	38.163469522450974	22.905533436947774
3	18.029064763080815	27.467356888890244	29.64447675214351	24.859101595885434
4	12.583831889118354	18.328502386072273	39.3968057959201	29.690859928889267
5	14.236593741160583	36.899718051115784	35.271440433355735	13.592247774367896
6	29.594139599675167	40.14319475155346	17.195232113582513	13.067433535188863
7	27.42781713166435	31.793614633359997	23.39552227457016	17.383045960405497
8	24.5262072552413	35.703944547011254	20.661956366357142	19.107891831390308
9	26.09882506090643	15.154524412758565	20.38578636973323	38.36086415660177
10	15.270406316628293	27.93696754394236	33.97788206811179	22.814744071317556
11	34.075666929251206	24.100850713084316	23.644166517125278	18.1793158405392
12	22.971990644285135	25.875881660548146	30.099792264198577	21.052335430968146
13	27.931644884315794	22.460559092167173	26.229762487719867	23.378033535797165
14	22.28415094454397	21.069215865783814	27.656995647585187	28.98963754208703
15	23.800500634156876	28.86295824297485	25.029578779924755	22.306962342943525
16	23.667434143492823	28.24811501811225	24.943503769963776	23.140947068431153
17	22.883178266516214	27.25855655553967	25.997542452012425	23.860722725931694
18	22.861431400041972	26.88657868563764	28.118698351192123	22.133291563128264
19	25.457976841868344	26.159807532627905	27.21050054291128	21.17171508259247
20	24.497616969247193	26.42016162636146	27.521952169059837	21.56026923533151
21	22.9312342791446	27.2231228500257	27.331400954428908	22.51424191640079
22	23.3406228424219	26.26762940906312	27.445001718458677	22.946746030056296
23	22.505117357040966	26.765678274120013	28.324153012777426	22.4050513560616
24	22.792084748907335	27.734706478132992	26.348533835386867	23.124674937572806
25	23.114789998266332	27.25399427585976	27.853934053767986	21.777281672105918
26	23.805367065815446	26.89646362494411	27.074392532460617	22.22377677677982
27	23.199648400312668	26.831223025521396	26.535131074295204	23.433997499870735
28	22.765927678742514	26.512927979852975	28.070338186585076	22.65080615481944
29	24.084122354257975	26.929768266607457	27.203961275370077	21.78214810376449
30	23.77130204420545	26.520835931298155	27.10876170604928	22.59910031844712
31	23.037687471675845	26.185964602792723	27.575330841314788	23.20101708421664
32	22.173591700300808	26.658312625652787	27.661101699297102	23.506993974749303
33	22.5423759744269	26.27234376473236	28.241575750571045	22.94370451026969
34	23.47901199271252	26.246642922535536	27.9076168780016	22.36672820675035
35	23.252266692620967	27.220385482217758	26.72994041662738	22.797407408533896
36	23.50988341854658	26.65101297816493	26.552619813068194	23.2864837902203
37	23.202233692131287	26.119051167487374	27.49716378279899	23.181551357582357
38	23.093803511738745	26.403585343524455	26.88308093788304	23.61953020685376
39	22.67802775690957	26.570716855798505	27.66034131935045	23.09091406794147
40	23.34898702183507	27.177956281194586	27.261445999336946	22.211610697633393
41	23.07920421676303	26.286943059708072	27.273460002494048	23.36039272103485
42	22.265141445877674	27.962516310149855	26.941934345753886	22.83040789821858
43	23.844298519084013	25.909794606168813	26.857228019696883	23.38867885505029
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	36.0
2	50.0
3	162.0
4	274.0
5	274.0
6	351.5
7	429.0
8	418.5
9	408.0
10	532.5
11	657.0
12	657.0
13	1087.0
14	1517.0
15	2776.0
16	4035.0
17	4503.0
18	4971.0
19	4971.0
20	4644.0
21	4317.0
22	4892.0
23	5467.0
24	6821.5
25	8176.0
26	8176.0
27	10101.5
28	12027.0
29	14670.0
30	17313.0
31	20295.0
32	23277.0
33	23277.0
34	29704.0
35	36131.0
36	39757.0
37	43383.0
38	45284.5
39	47186.0
40	47186.0
41	48678.0
42	50170.0
43	51579.0
44	52988.0
45	51809.5
46	50631.0
47	50631.0
48	48354.0
49	46077.0
50	46742.0
51	47407.0
52	47248.5
53	47090.0
54	47090.0
55	45120.5
56	43151.0
57	36132.5
58	29114.0
59	26851.5
60	24589.0
61	24589.0
62	22430.5
63	20272.0
64	15768.5
65	11265.0
66	9444.0
67	7623.0
68	7623.0
69	6609.0
70	5595.0
71	5448.5
72	5302.0
73	4657.5
74	4013.0
75	4013.0
76	2552.0
77	1091.0
78	877.5
79	664.0
80	525.0
81	386.0
82	386.0
83	293.0
84	200.0
85	177.5
86	155.0
87	120.0
88	85.0
89	85.0
90	64.0
91	43.0
92	28.5
93	14.0
94	7.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	657566.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.838023895733286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98870973580335	45.96311523679385
2	7.091686707394025	7.494214234126793
3	1.897815333101291	3.008304357602851
4	0.8708879680996228	1.8406399707581793
5	0.5623794402901341	1.4857509152259611
6	0.3656817337301296	1.1593140111039457
7	0.26796608038133013	0.9911158710904298
8	0.19805879986622976	0.8372028480073684
9	0.16478421199323498	0.7836184917844318
>10	1.2740830006604802	13.65964543575055
>50	0.17838899494291627	6.672979109964346
>100	0.1305071615074412	12.362967452709572
>500	0.007591020579851049	2.4556241067161118
>1k	0.0014598116499713555	1.2855079583657092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2941	0.4472554846205552	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2161	0.32863621294288325	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1123	0.17078133601798146	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1096	0.16667528430606204	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1012	0.15390090120231278	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	868	0.13200195873874257	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	798	0.12135663948561817	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	788	0.11983587959231469	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	784	0.1192275756349933	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	705	0.10721357247789577	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	703	0.10690942049923506	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	676	0.10280336878731565	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	662	0.10067430493669077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.520759893303486E-4	0.0	0.0
3	0.0	0.0	1.520759893303486E-4	0.0	0.0
4	0.0	0.0	4.5622796799104574E-4	0.0	0.0
5	0.0	0.0	4.5622796799104574E-4	1.520759893303486E-4	0.0
6	0.0	0.0	0.00106453192531244	1.520759893303486E-4	0.0
7	0.0	0.0	0.0038018997332587147	1.520759893303486E-4	0.0
8	0.0	0.0	0.00425812770124976	1.520759893303486E-4	0.0
9	0.0	0.0	0.0050185076479015035	9.124559359820915E-4	0.0
10	0.0	0.0	0.005626811605222898	9.124559359820915E-4	0.0
11	0.0	0.0	0.008060027434508475	0.00106453192531244	0.0
12	0.0	0.0	0.008972483370490567	0.0012166079146427888	0.0
13	0.0	0.0	0.009276635349151264	0.0012166079146427888	0.0
14	0.0	0.0	0.009884939306472659	0.0012166079146427888	0.0
15	0.0	0.0	0.010341167274463704	0.0012166079146427888	0.0
16	0.0	0.0	0.010645319253124402	0.0012166079146427888	0.0
17	0.0	0.0	0.010645319253124402	0.0013686839039731373	0.0
18	1.520759893303486E-4	0.0	0.01079739524245475	0.0013686839039731373	0.0
19	1.520759893303486E-4	0.0	0.010949471231785099	0.0013686839039731373	0.0
20	1.520759893303486E-4	0.0	0.011101547221115447	0.0013686839039731373	0.0
21	1.520759893303486E-4	0.0	0.011709851178436842	0.001824911871964183	0.0
22	1.520759893303486E-4	0.0	0.01186192716776719	0.00212906385062488	0.0
23	1.520759893303486E-4	0.0	0.012166079146427887	0.002281139839955229	0.0
24	1.520759893303486E-4	0.0	0.012166079146427887	0.0024332158292855776	0.0
25	1.520759893303486E-4	0.0	0.012166079146427887	0.002889443797276623	0.0
26	1.520759893303486E-4	0.0	0.012166079146427887	0.0031935957759373205	0.0
27	1.520759893303486E-4	0.0	0.012166079146427887	0.0050185076479015035	0.0
28	1.520759893303486E-4	0.0	0.012166079146427887	0.012622307114418934	0.0
29	1.520759893303486E-4	0.0	0.012318155135758235	0.02904651396209658	0.0
30	1.520759893303486E-4	0.0	0.012318155135758235	0.051401684393657825	0.0
31	1.520759893303486E-4	0.0	0.012318155135758235	0.10067430493669076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAGAC	40	5.936321E-5	27.750002	3
CCTTCCG	35	8.865344E-4	26.42857	8
TGCGGTA	60	1.3359531E-6	24.666668	36
GTATTGC	40	0.0019303125	23.125002	1
CGAGTCG	80	2.72139E-8	23.125002	21
ATTTGCG	40	0.0019303125	23.125002	16
GGTATCA	670	0.0	22.64179	1
GTATCAA	1785	0.0	22.593838	1
TTGCGCG	45	0.0038240938	20.555555	18
GTCAACC	160	1.8189894E-12	18.5	15
TTAACGG	210	0.0	18.5	35
TAGCACC	50	0.0070317597	18.499998	4
CCGCGCG	50	0.0070317597	18.499998	30
GTACATG	305	0.0	18.196722	1
CATGCTA	245	0.0	18.12245	4
GCGAACT	250	0.0	17.76	28
TAACGGC	220	0.0	17.65909	36
GCACCGC	220	0.0	17.65909	10
AGTCGCC	95	3.6016409E-6	17.526316	23
GACGTGC	85	2.720248E-5	17.411764	32
>>END_MODULE